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| 4. |
- Maisnier-Patin, S, et al.
(författare)
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RecA-mediated rescue of Escherichia coli strains with replication forks arrested at the terminus
- 2001
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Ingår i: JOURNAL OF BACTERIOLOGY. - : AMER SOC MICROBIOLOGY. - 0021-9193. ; 183:20, s. 6065-6073
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Tidskriftsartikel (refereegranskat)abstract
- The recombinational rescue of chromosome replication was investigated in Escherichia coli strains with the unidirectional origin oriR1, from the plasmid R1, integrated within oriC in clockwise (intR1(CW)) or counterclockwise (intR1(CC)) orientations. Only
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| 5. |
- Sanyal, Suparna, et al.
(författare)
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23S rRNA assisted folding of cytoplasmic malate dehydrogenase is distinctly different from its self-folding
- 2002
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Ingår i: Nucleic Acids Research. - : Oxford University Press (OUP). - 1362-4962. ; 30:11, s. 2390-2397
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Tidskriftsartikel (refereegranskat)abstract
- The role of the 50S particle of Escherichia coli ribosome and its 23S rRNA in the refolding and subunit association of dimeric porcine heart cytoplasmic malate dehydrogenase (s-MDH) has been investigated. The self-reconstitution of s-MDH is governed by two parallel pathways representing the folding of the inactive monomeric and the dimeric intermediates. However, in the presence of these folding modulators, only one first order kinetics was observed. To understand whether this involved the folding of the monomers or the dimers, subunit association of s-MDH was studied using fluorescein-5-isothiocyanate-rhodamine-isothiocyanate (FITC-RITC) fluorescence energy transfer and chemical crosslinking with gluteraldehyde. The observation suggests that during refolding the interaction of the unstructured monomers of s-MDH with these ribosomal folding modulators leads to very fast formation of structured monomers that immediately dimerise. These inactive dimers then fold to the native ones, which is the rate limiting step in 23S or 50S assisted refolding of s-MDH. Furthermore, the sequential action of the two fragments of domain V of 23S rRNA has been investigated in order to elucidate the mechanism. The central loop of domain V of 23S rRNA (RNA1) traps the monomeric intermediates, and when they are released by the upper stem-loop region of the domain V of 23S rRNA (RNA2) they are already structured enough to form dimeric intermediates which are directed towards the proper folding pathway.
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- Arrow, K, et al.
(författare)
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Managing Ecosystem Resources
- 2000
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Ingår i: Environmental Science & Technology. ; 34, s. 1401-1406
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Tidskriftsartikel (refereegranskat)
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| 7. |
- Nordstrom, K, et al.
(författare)
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Partitioning of the Escherichia coli chromosome: Superhelicity and condensation
- 2001
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Ingår i: BIOCHIMIE. - : EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER. - 0300-9084. ; 83:1, s. 41-48
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Tidskriftsartikel (refereegranskat)abstract
- Segregation in Escherichia coli, the process of separating the replicated chromosomes into daughter progeny cells, seems to start long before the duplication of the genome reaches completion. Soon after initiation in mid-cell region, the daughter oriCs ra
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- Tao, WT, et al.
(författare)
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Plasmid R1 is present as clusters in the cells of Escherichia coli
- 2000
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Ingår i: PLASMID. - : ACADEMIC PRESS INC. - 0147-619X. ; 43:3, s. 200-204
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Tidskriftsartikel (refereegranskat)abstract
- Fluorescence microscopy was used to determine the location(s) of the replication origin of plasmid R1 in exponentially growing cells of Escherichia coli. The number of oriR1 foci per cell was smaller than the number of R1 copies per cell and was found to
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| 10. |
- Weitao, T, et al.
(författare)
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Escherichia coli cell cycle control genes affect chromosome superhelicity
- 2000
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Ingår i: EMBO REPORTS. - : OXFORD UNIV PRESS. - 1469-221X. ; 1:6, s. 494-499
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Tidskriftsartikel (refereegranskat)abstract
- We have used ethidium bromide titration for direct measurement of the changes in the negative supercoiling of Escherichia coli chromosome caused by mutations inactivating the cell cycle functions mukB and seqA. The amounts of the intercalative agent requi
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