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Characterization of...
Characterization of a mouse monoclonal IgG3 antibody to the tumor-associated globo H structure produced by immunization with a synthetic glycoconjugate.
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Kudryashov, V (författare)
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Ragupathi, G (författare)
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Kim, I J (författare)
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visa fler...
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- Breimer, Michael, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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Danishefsky, S J (författare)
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Livingston, P O (författare)
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Lloyd, K O (författare)
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visa färre...
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(creator_code:org_t)
- 1998
- 1998
- Engelska.
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Ingår i: Glycoconjugate journal. - 0282-0080. ; 15:3, s. 243-9
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Globo H (Fuc alpha1 --> 2Galbeta1 --> 3GalNAcbeta1 --> 3Gal alpha1 --> 4Galbeta1 --> 4Glc) is a carbohydrate structure that shows enhanced expression in many human carcinomas. From mice immunized with a globo H-KLH (keyhole limpet hemocyanin) synthetic conjugate an IgG3 monoclonal antibody (mAb VK-9) was derived that recognizes the globo H structure. Serological analysis showed that the minimal structure recognized by this mAb was the tetrasaccharide sequence Fuc alpha1 --> 2Galbeta1 --> 3GalNAcbeta1 --> 3Gal. An isomeric structure with an internal alphaGalNAc linkage was also recognized but less efficiently. mAb VK-9 did not react with many related structures, such as galactosylgloboside, globoside, H type 1, H type 2 blood group structures or fucosyl-gangliotetraosyl ceramide, but did react weakly with globo A ceramide. Not only did mAb VK-9 react with carbohydrate-protein conjugates but it could also recognize globo H-ceramide and human tumor cells expressing globo H. These results suggest that globo H-KLH could be explored as a vaccine in the treatment of carcinoma patients.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- Animals
- Antibodies
- Monoclonal
- immunology
- Antibody Specificity
- Antigens
- Tumor-Associated
- Carbohydrate
- chemistry
- Cancer Vaccines
- chemistry
- isolation & purification
- Carbohydrate Sequence
- Female
- Glycoconjugates
- chemistry
- immunology
- Hemocyanin
- immunology
- Humans
- Immunization
- Immunoglobulin G
- immunology
- Mice
- Molecular Sequence Data
- Vaccines
- Conjugate
- chemistry
- isolation & purification
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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