| 1. |
- Sodergren, Erica, et al.
(författare)
-
The genome of the sea urchin Strongylocentrotus purpuratus.
- 2006
-
Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 1095-9203 .- 0036-8075. ; 314:5801, s. 941-52
-
Tidskriftsartikel (refereegranskat)abstract
- We report the sequence and analysis of the 814-megabase genome of the sea urchin Strongylocentrotus purpuratus, a model for developmental and systems biology. The sequencing strategy combined whole-genome shotgun and bacterial artificial chromosome (BAC) sequences. This use of BAC clones, aided by a pooling strategy, overcame difficulties associated with high heterozygosity of the genome. The genome encodes about 23,300 genes, including many previously thought to be vertebrate innovations or known only outside the deuterostomes. This echinoderm genome provides an evolutionary outgroup for the chordates and yields insights into the evolution of deuterostomes.
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
- Elsik, Christine G., et al.
(författare)
-
The Genome Sequence of Taurine Cattle : A Window to Ruminant Biology and Evolution
- 2009
-
Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 324:5926, s. 522-528
-
Tidskriftsartikel (refereegranskat)abstract
- To understand the biology and evolution of ruminants, the cattle genome was sequenced to about sevenfold coverage. The cattle genome contains a minimum of 22,000 genes, with a core set of 14,345 orthologs shared among seven mammalian species of which 1217 are absent or undetected in noneutherian (marsupial or monotreme) genomes. Cattle-specific evolutionary breakpoint regions in chromosomes have a higher density of segmental duplications, enrichment of repetitive elements, and species-specific variations in genes associated with lactation and immune responsiveness. Genes involved in metabolism are generally highly conserved, although five metabolic genes are deleted or extensively diverged from their human orthologs. The cattle genome sequence thus provides a resource for understanding mammalian evolution and accelerating livestock genetic improvement for milk and meat production.
|
|
| 5. |
- Giordanetto, Fabrizio, et al.
(författare)
-
Discovery of cyclopentane- and cyclohexane-trans-1,3-diamines as potent melanin-concentrating hormone receptor 1 antagonists
- 2007
-
Ingår i: Bioorganic & Medicinal Chemistry Letters. - : Elsevier BV. - 0960-894X. ; 17:15, s. 4232-41
-
Tidskriftsartikel (refereegranskat)abstract
- We herein report the optimization of cyclopentane- and cyclohexane-1,3-diamine derivatives as novel and potent MCH-R1 antagonists. Structural modifications of the 2-amino-quinoline and thiophene moieties found in the initial lead compound served to improve its metabolic stability profile and MCH-R1 affinity, and revealed unprecedented SAR when compared to other 2-amino-quinoline-containing MCH-R1 antagonists.
|
|
| 6. |
- Goodfellow, IG, et al.
(författare)
-
Inhibition of coxsackie B virus infection by soluble forms of its receptors: Binding affinities, altered particle formation, and competition with cellular receptors
- 2005
-
Ingår i: Journal of Virology. - 1098-5514. ; 79:18, s. 12016-12024
-
Tidskriftsartikel (refereegranskat)abstract
- We previously reported that soluble decay-accelerating factor (DAF) and coxsackievirus-adenovirus receptor (CAR) blocked coxsackievirus 133 (CVB3) myocarditis in mice, but only soluble CAR blocked CVB3-mediated pancreatitis. Here, we report that the in vitro mechanisms of viral inhibition by these soluble receptors also differ. Soluble DAF inhibited virus infection through the formation of reversible complexes with CVB3, while binding of soluble CAR to CVB induced the formation of altered (A) particles with a resultant irreversible loss of infectivity. A-particle formation was characterized by loss of VP4 from the virions and required incubation of CVB3-CAR complexes at 37 degrees C. Dimeric soluble DAF (DAF-Fc) was found to be 125-fold-more effective at inhibiting CVB3 than monomeric DAF, which corresponded to a 100-fold increase in binding affinity as determined by surface plasmon resonance analysis. Soluble CAR and soluble dimeric CAR (CAR-Fc) bound to CVB3 with 5,000- and 10,000-fold-higher affinities than the equivalent forms of DAF. While DAF-Fc was 125-fold-more effective at inhibiting virus than monomeric DAF, complement regulation by DAF-Fc was decreased 4 fold. Therefore, while the virus binding was a cooperative event, complement regulation was hindered by the molecular orientation of DAF-Fc, indicating that the regions responsible for complement regulation and virus binding do not completely overlap. Relative contributions of CVB binding affinity, receptor binding footprint on the virus capsid, and induction of capsid conformation alterations for the ability of cellular DAF and CAR to act as receptors are discussed.
|
|
| 7. |
- Haas, Brian J., et al.
(författare)
-
Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans
- 2009
-
Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 461:7262, s. 393-398
-
Tidskriftsartikel (refereegranskat)abstract
- Phytophthora infestans is the most destructive pathogen of potato and a model organism for the oomycetes, a distinct lineage of fungus-like eukaryotes that are related to organisms such as brown algae and diatoms. As the agent of the Irish potato famine in the mid-nineteenth century, P. infestans has had a tremendous effect on human history, resulting in famine and population displacement(1). To this day, it affects world agriculture by causing the most destructive disease of potato, the fourth largest food crop and a critical alternative to the major cereal crops for feeding the world's population(1). Current annual worldwide potato crop losses due to late blight are conservatively estimated at $6.7 billion(2). Management of this devastating pathogen is challenged by its remarkable speed of adaptation to control strategies such as genetically resistant cultivars(3,4). Here we report the sequence of the P. infestans genome, which at similar to 240 megabases (Mb) is by far the largest and most complex genome sequenced so far in the chromalveolates. Its expansion results from a proliferation of repetitive DNA accounting for similar to 74% of the genome. Comparison with two other Phytophthora genomes showed rapid turnover and extensive expansion of specific families of secreted disease effector proteins, including many genes that are induced during infection or are predicted to have activities that alter host physiology. These fast-evolving effector genes are localized to highly dynamic and expanded regions of the P. infestans genome. This probably plays a crucial part in the rapid adaptability of the pathogen to host plants and underpins its evolutionary potential.
|
|
| 8. |
- Johansson, Kristin, 1979, et al.
(författare)
-
Environmental impact of the EPSPEX system. Part 1: Life cycle assessment of polystyrene insulated district heating systems with PEX fluid pipes
- 2005
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The EPSPEX system is a distribution system for district heat with media pipes of cross-linked polyethylene (PEX) insulated by blocks of expanded polystyrene (EPS). In this low-pressure distribution system two pipes are used for a space heating circuit, having layers of EVAL to hinder oxygen diffusion, whereas two pipes without diffusion barrier are used for delivery of hot tap water and warm water circulation. The four pipes are placed in the insulating blocks of EPS. Environmental impacts from production, laying and use of an EPSPEX district heat distribution system have been investigated using life cycle methodology. The distribution system is a secondary system developed specially for areas with low heat density. The case studied is the installation and use of an EPSPEX system in Vråen, Värnamo, Sweden. The environmental impacts are described in four categories:climate change, acidification, eutrophication and use of finite resources. Four weighting methods have also been used in the evaluation: EPS2000, Ecoscarcity, ExternE and EcoIndicator99. While carefully considering the precise circumstances of this study, the findings can be adapted for using the EPSPEX district heat distribution systems in other areas.During this study the question was raised of how this district heat delivery system compares with systems that use conventional district heating pipes. This has beenfurther investigated and is reported in the second part of this report: Miljöbelastning från EPSPEX-systemet. Del 2: Konventionella twinrör eller EPSPEX vad är bäst urmiljösynpunkt. In the second part the environmental impacts from the EPSPEX system have been compared with the impacts from conventional twin pipes (polyurethane insulated steel media pipes) used in a comparable setting. A discussion on the environmental choice between media pipes of PEX and steel can also be found in the second part. It should be noted that the two systems for district heat distribution are not fully comparable in function. The EPSPEX system has stricter limitations regarding pressure and temperature compared with the conventional system. The EPSPEX system is also limited in use for well drained ground conditions above the water table. Conventional district heating pipes do not have this limitation. The most important possibility to decrease the environmental impacts from the EPSPEX system is to increase the insulation, this even though the distribution systemis comparably well insulated to start with. The environmental life cycle impacts of the distribution pipe system production, laying and use are predominated by the extra heat that has to be generated to cover the heat losses from the system. When this report was written, measurements from systems in use to confirm the model calculation of heat losses are still lacking. More fine-tuned results can be achieved when studies of the EPSPEX system in use have been concluded.Among different components in the EPSPEX system the impacts from production and transport of EPS-blocks make the major impact. Thus, it is important to utilize the polystyrene material as efficiently as possible, minimizing spillage, and to ensure that unavoidable spillage is recycled as material. However, the environmental impacts from production of the insulating blocks are well outweighed by the decreased environmental impacts gained by using the insulation. In product development it is thus important to ensure that the insulating capacity of the EPSPEX system is never decreased.Machines used for excavating the pipe trenches combust diesel oil, and the production and use of this diesel oil generates a substantial part of the environmental impacts byseveral of the assessment methods used. If possible, it is desirable to further minimize the excavation work and to use mini excavators and low emitting machines (especially regarding nitrous oxides). The brass swaged coupling used to connect the PEX-pipes, gives a strong impact regarding acidification and the two weighting methods EPS2000 and Ecoscarcity, especially when compared to the very small volume fraction these devices constitute. It would be advisable to study if the devices could be made from other material or from recycled material.
|
|
| 9. |
- Johansson, Kristin, 1979, et al.
(författare)
-
Environmental impact of the EPSPEX system. Part 2: Traditional twin pipes or EPSPEX for district heat distribution - what is best from an environmental point of view?
- 2005
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- In this study the life-cycle environmental impacts from an EPSPEX system for distribution of district heat (a four-pipe system with centrally generated hot tap water) are compared with the environmental impacts of a twin-pipe system with steel media pipes, if installed in the same area. A life cycle assessment of the EPSPEX system constructed in Vråen, Värnamo, Sweden, has been reported in Miljöbelastning från EPSPEX-systemet. Del 1: Livscykelanalys av polystyrenisolerat fjärrvärmesystemmed PEX-mediarör [1]. In that study the question was raised of how the EPSPEX system compares with a conventional twin-pipe system with polyurethane-insulated steel media pipes.In the present study the EPSPEX system in Vråen is compared with a hypothetical construction of a conventional twin pipe system in the same area. Two different fuel mixes for district heat generation have been studied to increase the understanding of the environmental performance of district heat distribution systems; the fuel mix used in Vråen (heat delivered from Värnamo Energi) and Swedish average district heat. How media pipes from the two different materials (PEX and steel) compare is discussed in a separate part. There are technical differences that must be kept in mind when the systems are compared in this way. The EPSPEX system is designed for low-pressure district heat systems and could only be used in such context. It is unadvisable to install the EPSPEX system below the ground water table; this is not a restriction for the twin pipe system. Under the conditions of this study the environmental performance of the EPSPEX system is better than the twin pipe system.The heat loss from the EPSPEX system is approximately 60% of the heat loss for a comparable twin pipe system. This is caused by a combination of thick insulation for the EPSPEX system and the possibility to summertime shut down the district heating circuit for this system and only use the hot tap-water circuit. Note, however, that the heat loss from the EPSPEX system is more uncertain compared to the twin-pipe system. From the characterization methods used, the production of the EPSPEX system gives a higher environmental impact compared to the twin pipe system. This heavily depends on the large amount of insulating material used. However, the environmental impacts from production of the insulation are more then outweighed by the emissionsavoided from heat generation due to lower heat loss. District heating sub centrals generates a notable part of the total environmental impact when using the weighing method EPS2000. Note that sub centrals are not technicallynecessary in low-pressure district heat systems, as the EPSPEX system. The comparison between different fuel mixes for heat generation indicates clearly that the heat generation is an important parameter for environmental performance for all district heating. It is further indicated that also biofuels must be used with sensible andenvironmentally friendly methods. The media pipes of PEX seem to give lower environmental impacts compared withmedia pipes of steel. However, the results differ between assessment methods used.
|
|
| 10. |
|
|
