| 1. |
- Coulon, Severine, et al.
(författare)
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Polymeric IgA1 controls erythroblast proliferation and accelerates erythropoiesis recovery in anemia
- 2011
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Ingår i: Nature Medicine. - : Springer Science and Business Media LLC. - 1546-170X .- 1078-8956. ; 17:11, s. 163-1456
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Tidskriftsartikel (refereegranskat)abstract
- Anemia because of insufficient production of and/or response to erythropoietin (Epo) is a major complication of chronic kidney disease and cancer. The mechanisms modulating the sensitivity of erythroblasts to Epo remain poorly understood. We show that, when cultured with Epo at suboptimal concentrations, the growth and clonogenic potential of erythroblasts was rescued by transferrin receptor 1 (TfR1)-bound polymeric IgA1 (pIgA1). Under homeostatic conditions, erythroblast numbers were increased in mice expressing human IgA1 compared to control mice. Hypoxic stress of these mice led to increased amounts of pIgA1 and erythroblast expansion. Expression of human IgA1 or treatment of wild-type mice with the TfR1 ligands pIgA1 or iron-loaded transferrin (Fe-Tf) accelerated recovery from acute anemia. TfR1 engagement by either pIgA1 or Fe-Tf increased cell sensitivity to Epo by inducing activation of mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) signaling pathways. These cellular responses were mediated through the TfR1-internalization motif, YXX Phi. Our results show that pIgA1 and TfR1 are positive regulators of erythropoiesis in both physiological and pathological situations. Targeting this pathway may provide alternate approaches to the treatment of ineffective erythropoiesis and anemia.
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| 2. |
- Edgar, Graham J., et al.
(författare)
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Global conservation outcomes depend on marine protected areas with five key features
- 2014
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 506:7487, s. 216-
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Tidskriftsartikel (refereegranskat)abstract
- In line with global targets agreed under the Convention on Biological Diversity, the number of marine protected areas (MPAs) is increasing rapidly, yet socio-economic benefits generated by MPAs remain difficult to predict and under debate(1,2). MPAs often fail to reach their full potential as a consequence of factors such as illegal harvesting, regulations that legally allow detrimental harvesting, or emigration of animals outside boundaries because of continuous habitat or inadequate size of reserve(3-5). Here we show that the conservation benefits of 87 MPAs investigated worldwide increase exponentially with the accumulation of five key features: no take, well enforced, old (>10 years), large (>100 km(2)), and isolated by deep water or sand. Using effective MPAs with four or five key features as an unfished standard, comparisons of underwater survey data from effective MPAs with predictions based on survey data from fished coasts indicate that total fish biomass has declined about two-thirds from historical baselines as a result of fishing. Effective MPAs also had twice as many large (>250 mm total length) fish species per transect, five times more large fish biomass, and fourteen times more shark biomass than fished areas. Most (59%) of the MPAs studied had only one or two key features and were not ecologically distinguishable from fished sites. Our results show that global conservation targets based on area alone will not optimize protection of marine biodiversity. More emphasis is needed on better MPA design, durable management and compliance to ensure that MPAs achieve their desired conservation value.
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| 3. |
- Thapper, Anders, et al.
(författare)
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Copper-substituted forms of the wild type and C42A variant of rubredoxin
- 2013
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Ingår i: Journal of Inorganic Biochemistry. - : Elsevier BV. - 0162-0134 .- 1873-3344. ; 127, s. 232-237
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Tidskriftsartikel (refereegranskat)abstract
- In order to gain insights into the interplay between Cu(I) and Cu(II) in sulfur-rich protein environments, the first preparation and characterization of copper-substituted forms of the wild-type rubredoxin (Rd) from Desulfovibrio vulgaris Hildenborough are reported, as well as those of its variant C42A-Rd. The initial products appear to be tetrahedral Cu-I(S-Cys) species for the wild type (n = 4) and the variant C42A (n = 3, with an additional unidentified ligand). These species are unstable to aerial oxidation to products, whose properties are consistent with square planar Cull(S-Cys)n species. These Cu(II) intermediates are susceptible to auto-reduction by ligand S-Cys to produce stable Cu(l) final products. The original Cu(I) center in the wild-type system can be regenerated by reduction, suggesting that the active site can accommodate Cu-I(S-Cys)2 and Cys-S-S-Cys fragments in the final product. The absence of one S-Cys ligand prevents similar regeneration in the C42A-Rd system. These results emphasize the redox instability of Cu-II-(S-Cys)(n) centers.
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| 4. |
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| 5. |
- Moura, Camilla C G, et al.
(författare)
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Evaluation of human polymorphonuclear behavior on textured titanium and calcium-phosphate coated surfaces
- 2013
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Ingår i: Biomedical Materials. - : Bibliopolis, Edizioni di Filosofia e Scienze. - 1748-6041 .- 1748-605X. ; 8:3
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Tidskriftsartikel (refereegranskat)abstract
- Few studies have evaluated the effects of titanium (Ti) surface modifications on polymorphonuclear neutrophils (PMNs). Human PMNs' viability and release of key mediators-such as IL1β, IL6, TNFα, IL12, IL10, IL4, TGFβ1, IL8, IP-10, and Mig-were evaluated on three different Ti surface treatments: (1) machined Ti; (2) alumina-blasted and acid-etched Ti (AB/AE); and (3) calcium phosphate coating of 300-500 nm by ion beam onto the AB/AE Ti surface (CaP). A polystyrene surface was used as a negative control. The PMNs were purified from whole human blood and cultured for 6 h. Cell viability was determined by flow cytometry, and the supernatant was evaluated to determine the levels of cytokines and chemokines. Results showed that the percentage of viable cells was significantly lower on the CaP surface compared to the control (p < 0.05) relative to the other groups. No differences in the levels of IL8, MIG, and IP10 were detected between groups. Significantly higher levels of IL1β (p = 0.046) and TNFα (p = 0.016) were detected for the CaP surfaces compared to AB/AE surface only. The levels of IL4, IL10, and TGFβ1 secreted from the PMNs in the CaP group were significantly lower than in the control and machined groups (p < 0.05) that were statistically comparable to AB/AE. Overall, the addition of a thin CaP coating to the AB/AE Ti surface influenced the secretion profile of pro-inflammatory cytokines due to the higher release of pro-inflammatory cytokines (IL1β and TNFα) on these surfaces.
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| 6. |
- Moura, Camilla G, et al.
(författare)
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Evaluation of osteogenic cell culture and osteogenic/peripheral blood mononuclear human cell co-culture on modified titanium surfaces
- 2013
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Ingår i: Biomedical Materials. - : Bibliopolis, Edizioni di Filosofia e Scienze. - 1748-6041 .- 1748-605X. ; 8:3
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Tidskriftsartikel (refereegranskat)abstract
- This study aimed to determine the effect of a bioactive ceramic coating on titanium in the nanothickness range on human osteogenic cells, peripheral blood mononuclear cells (PBMC) and on osteogenic cells co-cultured with PBMC without exogenous stimuli. Cell viability, proliferation, adhesion, cytokine release (IL1β, TGFβ1, IL10 and IL17) and intracellular stain for osteopontin and alkaline phosphatase were assessed. Morphologic evaluation showed smaller and less spread cell aspects in co-culture relative to osteogenic cell culture. Cell viability, proliferation and adhesion kinetics were differently influenced by surface texture/chemistry in culture versus co-culture. Cytokine release was also influenced by the interaction between mononuclear and osteogenic cells (mediators released by mononuclear cells acted on osteogenic cells and vice versa). In general, 'multi-cell type' interactions played a more remarkable role than the surface roughness or chemistry utilized on the in vitro cellular events related to initial stages of bone formation.
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| 7. |
- Ribeiro, Luis, et al.
(författare)
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A network inference tool for JADE-based systems
- 2014
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Ingår i: Proceedings - 2014 12th IEEE International Conference on Industrial Informatics, INDIN 2014. - : IEEE conference proceedings. - 9781479949052 ; , s. 398-403
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Konferensbidrag (refereegranskat)abstract
- This article describes the first version of a tool designed to infer the network characteristics of JADE-based multiagent systems. The rationale behind the tool is that systems in general and multiagent system in particular, often have some hidden dynamics that contribute to the emergence of desired and undesired characteristics. Traditional sniffing tools simply display the message exchange. The presented tool goes therefore beyond simple message sniffing and infers the agents' network based on the ongoing interactions and codifies it a format suitable for further processing in specialized network analysis tools. In particular the prosped tool identifies the most frequently used communication links and the messages associated with them. To demonstrate the behavior of the tool an exploratory system based on the Evolvable Production System paradigm is discussed and analyzed.
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| 8. |
- Sun, C., et al.
(författare)
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Comparison of velocity forecasting strategies for predictive control in HEVS
- 2014
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Ingår i: ASME 2014 Dynamic Systems and Control Conference, DSCC 2014; San Antonio; United States; 22 October 2014 through 24 October 2014. - 9780791846193 ; 2, s. Art. no. 6031-
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Konferensbidrag (refereegranskat)abstract
- The performance of model predictive control (MPC) for energy management in hybrid electric vehicles (HEVS) is strongly dependent on the projected future driving profile. This paper proposes a novel velocity forecasting method based on artificial neural networks (ANN). The objective is to improve the fuel economy of a power-split HEV in a nonlinear MPC framework. In this study, no telemetry or on-board sensor information is required. A comparative study is conducted between the ANNbased method and two other velocity predictors: generalized exponentially varying and Markov-chain models. The sensitivity of the prediction precision and computational cost on tuning parameters in examined for each forecasting strategy. Validation results show that the ANN-based velocity predictor exhibits the best overall performance with respect to minimizing fuel consumption.
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