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Träfflista för sökning "WFRF:(Murphy J) srt2:(2000-2004)"

Sökning: WFRF:(Murphy J) > (2000-2004)

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  • Dette, HH, et al. (författare)
  • Application of prototype flume tests for beach nourishment assessment
  • 2002
  • Ingår i: Coastal Engineering. - 0378-3839. ; 47:2, s. 137-177
  • Tidskriftsartikel (refereegranskat)abstract
    • The development of underwater profiles and sand losses from the dune respectively the beach by various water level and wave conditions has been studied worldwide in large wave flumes since 1955. Virtually all major results collected over the last decades are documented and described in this paper. Moreover, to this overview results from the MAST III-SAFE experiments, carried out in the Grosser Wellenkanal (GWK) in Hannover, are summarized and discussed in more detail. The tests were focused on beach and dune stability under given conditions with normal and raised water levels. The results are concentrated on surf zone profiles, beach slope and erosion. Furthermore, dune erosion with and without barriers, built into the dune face, and perched surf zone by means of an underwater sill were investigated.
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  • Balan, S, et al. (författare)
  • Metal chelate affinity precipitation of RNA and purification of plasmid DNA
  • 2003
  • Ingår i: Biotechnology Letters. - 1573-6776. ; 25:13, s. 1111-1116
  • Tidskriftsartikel (refereegranskat)abstract
    • The affinity of metal chelates for amino acids, such as histidine, is widely used in purifying proteins, most notably through six-histidine `tails'. We have found that metal affinity interactions can also be applied to separation of single-stranded nucleic acids through interactions involving exposed purines. Here we describe a metal affinity precipitation method to resolve RNA from linear and plasmid DNA. A copper-charged copolymer of N-isopropyl acrylamide (NIPAM) and vinyl imidazole (VI) is used to purify plasmid from an alkaline lysate of E. coli. The NIPAM units confer reversible solubility on the copolymer while the imidazole chelates metal ions in a manner accessible to interaction with soluble ligands. RNA was separated from the plasmid by precipitation along with the polymer in the presence of 800 mM NaCl. Bound RNA could be recovered by elution with imidazole and separated from copolymer by a second precipitation step. RNA binding showed a strong dependence on temperature and on the type of buffer used.
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