| 1. |
- Nyberg, Lena, 1979, et al.
(författare)
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A single-step competitive binding assay for mapping of single DNA molecules
- 2012
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 417:1, s. 404-408
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Tidskriftsartikel (refereegranskat)abstract
- Optical mapping of genomic DNA is of relevance for a plethora of applications such as scaffolding for sequencing and detection of structural variations as well as identification cif pathogens like bacteria and viruses. For future clinical applications it is desirable to have a fast and robust mapping method based on as few steps as possible. We here demonstrate a single-step method to obtain a DNA barcode that is directly visualized using nanofluidic devices and fluorescence microscopy. Using a mixture of YOYO-1, a bright DNA dye, and netropsin, a natural antibiotic with very high AT specificity, we obtain a DNA map with a fluorescence intensity profile along the DNA that reflects the underlying sequence. The netropsin binds to AT-tetrads and blocks these binding sites from YOYO-1 binding which results in lower fluorescence intensity from AT-rich regions of the DNA. We thus obtain a DNA barcode that is dark in AT-rich regions and bright in GC-rich regions with kilobasepair resolution. We demonstrate the versatility of the method by obtaining a barcode on DNA from the phage T4 that captures its circular permutation and agrees well with its known sequence.
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| 2. |
- Armstrong, Andrea, et al.
(författare)
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Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimers Disease
- 2014
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Ingår i: Neuromolecular medicine. - : Humana Press. - 1535-1084 .- 1559-1174. ; 16:1, s. 150-160
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Tidskriftsartikel (refereegranskat)abstract
- The success of future intervention strategies for Alzheimers disease (AD) will likely rely on the development of treatments starting early in the disease course, before irreversible brain damage occurs. The pre-symptomatic stage of AD occurs at least one decade before the clinical onset, highlighting the need for validated biomarkers that reflect this early period. Reliable biomarkers for AD are also needed in research and clinics for diagnosis, patient stratification, clinical trials, monitoring of disease progression and the development of new treatments. Changes in the lysosomal network, i.e., the endosomal, lysosomal and autophagy systems, are among the first alterations observed in an AD brain. In this study, we performed a targeted search for lysosomal network proteins in human cerebrospinal fluid (CSF). Thirty-four proteins were investigated, and six of them, early endosomal antigen 1 (EEA1), lysosomal-associated membrane proteins 1 and 2 (LAMP-1, LAMP-2), microtubule-associated protein 1 light chain 3 (LC3), Rab3 and Rab7, were significantly increased in the CSF from AD patients compared with neurological controls. These results were confirmed in a validation cohort of CSF samples, and patients with no neurochemical evidence of AD, apart from increased total-tau, were found to have EEA1 levels corresponding to the increased total-tau levels. These findings indicate that increased levels of LAMP-1, LAMP-2, LC3, Rab3 and Rab7 in the CSF might be specific for AD, and increased EEA1 levels may be a sign of general neurodegeneration. These six lysosomal network proteins are potential AD biomarkers and may be used to investigate lysosomal involvement in AD pathogenesis.
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| 3. |
- Hjalmarsson, Linnea, et al.
(författare)
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Pathways to a renewable road transport system in Stockholm 2030
- 2011
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- In order to mitigate global climate change, anthropogenic emissions of fossil carbon dioxide (CO2) need to be cut drastically. Road transport is a major source of CO2 emissions, and in urban areas road transport also involves problems such as congestion, noise and particle emissions. Stockholm, the Swedish capital and one of the busiest regions in Sweden, has the ambition to be a pioneer in addressing environmental problems; CO2 emissions in particular. One of the political visions incorporated in Stockholm’s environmental work is to achieve a practically renewable transport system by 2030. This study investigates if there are favourable conditions to achieve a renewable road transport system in Stockholm by 2030. Three aspects are considered; technology, private economy and regional planning policy. The study is based on three sub-studies, one for each aspect, and conclusions are drawn from the integration of the sub-studies. A scenario assessment implies that the technology to transit to a completely renewable road transport system could exist, and that a mix of technologies would be preferable. Cost optimisations show that renewable fuels and electric vehicles are cost-competitive given certain incentives. Hence, private persons could shift their transportation choices towards alternative vehicles and fuels. Interviews with regional institutional actors and analysis of regional planning documents reveal that integrating energy and transport systems in planning policy could enable the transition to a renewable road transport system in Stockholm. The work has been carried out under the auspices of The Energy Systems Programme (primarily financed by The Swedish Energy Agency). The study concludes that favourable conditions for a renewable road transport system do exist. However, the main challenge is to coordinate the simultaneous implementation of necessary measures and the study shows that this is best organised at a regional level.
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| 4. |
- Lyngfelt, Benjamin, 1968, et al.
(författare)
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Ett svenskt konstruktikon. Grammatik möter lexikon
- 2014
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Ingår i: Svenskans beskrivning : Förhandlingar vid Trettiotredje sammankomsten för svenskans beskrivning. Helsingfors den 15–17 maj 2013. - 1795-4428. - 9789515101204 ; 33, s. 268-279, s. 268-279
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Konferensbidrag (refereegranskat)
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| 5. |
- Olsson, Linda, et al.
(författare)
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Policy for biomass utilisation in energy and transport systems : The case of biogas in Stockholm, Sweden
- 2012
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Ingår i: World Renewable Energy Forum(WREF) 2012. Including 41st ASES Annual Conference, 37th National PassiveSolar Conference, 7th Renewable Energy Policy and MarketingConference, World Renewable Energy Congress XII, andColorado Renewable Energy Society (CRES) Annual Conference. Denver, Colorado, USA13 – 17 May 2012, Volume 1 of 6. - 9781622760923 ; , s. 4758-4765
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- This interdisciplinary paper explains how biogas policy processes affect energy and transport systems in Stockholm, Sweden. The aim is to discuss future implications of the political biogas agenda, and analyse how municipal organic waste should be utilised to achieve greenhouse gas emission reductions. An energy systems analysis illustrates the environmental impact of biogas production and utilisation, while planning practice and political attitudes are studied in interviews with policy-makers. Conclusions include that demand for biogas as vehicle fuel and the influence of particular actors has led to an extensive political campaign for biogas. However, to maximise emission reductions, Stockholm’s waste should ideally be used to produce electricity, not biogas. With the current narrow system perspective and lack of long-term strategies, increased utilisation of renewable fuels in the transport system may be impeded.
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| 6. |
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| 7. |
- Sköldberg, Emma, 1968, et al.
(författare)
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Between Grammars and Dictionaries: a Swedish Constructicon
- 2013
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Ingår i: Kosem, I., Kallas, J., Gantar, P., Krek, S., Langemets, M., Tuulik, M. (eds.) 2013. Electronic lexicography in the 21st century: thinking outside the paper. Proceedings of the eLex 2013 conference, 17-19 October 2013, Tallinn, Estonia. Ljubljana/Tallinn: Trojina, Institute for Applied Slovene Studies/Eesti Keele Instituut.. ; , s. 310-327, s. 310-327
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- This paper introduces the Swedish Constructicon (SweCxn), a database of Swedish constructions currently under development. We also present a small study of the treatment of constructions in Swedish (paper) dictionaries, thus illustrating the need for a constructionist approach, and discuss three different methods used to identify potential constructions for inclusion in the constructicon. SweCxn is a freely available electronic resource, with a particular focus on semi-general linguistic patterns of the type that are difficult to account for from a purely lexicographic or a purely grammatical perspective, and which therefore have tended to be neglected in both dictionaries and grammars. Far from being a small set of borderline cases, such constructions are both numerous and common. They are also quite problematic for second language acquisition as well as LT applications. Accordingly, various kinds of multi-word units have received more attention in recent years, not least from a lexicographic perspective. The coverage, however, is only partial, and the productivity of many constructions is hard to capture from a lexical viewpoint. To identify constructions for SweCxn, we use a combination of methods, such as working from existing construction descriptions for Swedish and other languages, applying LT tools to discover recurring patterns in texts, and extrapolating constructional information from dictionaries.
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