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Träfflista för sökning "WFRF:(Sheng Hung Wang) srt2:(2020-2023)"

Sökning: WFRF:(Sheng Hung Wang) > (2020-2023)

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Justino, Flavio, et al. (författare)
  • Influence of local scale and oceanic teleconnections on regional fire danger and wildfire trends
  • 2023
  • Ingår i: Science of the Total Environment. - 0048-9697 .- 1879-1026. ; 883
  • Tidskriftsartikel (refereegranskat)abstract
    • Studies and observations have pointed out that recent wildfires have been more severe and burned area is increasing in tropical regions. The current study aims at investigating the influence of oceanic climate modes and their teleconnection on global fire danger and trends in the 1980–2020 interval. Disentangling these trends demonstrates that across the extratropics they are primarily related to increases in temperature, whereas in the tropics changes in short-term precipitation distribution dominates the trends. Moreover, the environmental impact of short-term precipitation is dependent on local vegetation type and tightly related to oceanic temperatures far from the burned areas. Indeed, in the 2001–2020 period, a warmer tropical North Atlantic was associated with more fires in the Amazon and Africa, whereas ENSO has weakened the fire activity in equatorial Africa. The remarkable impact of oceanic modes of climate variability in inducing environmental conditions conducive to fires, has particular relevance for the seasonal spatiotemporal wildfire forecasts. Although local aspects are crucial for fire management, long-term predictions should take into account the behavior of potential climate drivers located far from the region of interest. Such teleconnections can be identified ahead of local weather anomalies.
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