| 1. |
- Thornton, David, et al.
(författare)
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Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation : evidence for an association involving the MUC5B mucin
- 2001
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Ingår i: Glycobiology. - : Oxford Journals. - 0959-6658 .- 1460-2423. ; 11:11, s. 969-977
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Tidskriftsartikel (refereegranskat)abstract
- Rate-zonal centrifugation of a reduced and alkylated respiratory mucin preparation identified a protein-rich fraction. This was subjected to trypsin treatment and one of the many liberated peptides was purified and its N-terminal sequence determined. The peptide was identical to a 14 amino acid sequence from the scavenger receptor cysteine-rich domain containing glycoprotein gp-340. A polyclonal antiserum, raised against the peptide, stained the serous cells in the submucosal glands of human tracheal tissue. The glycoprotein was purified from respiratory mucus by density-gradient centrifugation, gel chromatography, and anion exchange chromatography. The molecule exhibited a heterogeneous distribution of buoyant density (1.28-1.46 g/ml) that overlapped with the gel-forming mucins, was included on Sepharose CL-2B and was quite highly anionic. SDS-PAGE indicated a mass greater than 208 kDa and measurements performed across the molecular size distribution indicated an average M(r) of 5 x 10(5) with a range of M(r) from 2 x 10(5) to 1 x 10(6). Gel chromatography of respiratory mucus extracts ("associative" and "dissociative") indicated that this glycoprotein forms complexes that may involve the large gel-forming mucins MUC5AC and MUC5B. Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins.
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| 2. |
- Thornton, K., et al.
(författare)
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Modelling the evolution of phase boundaries in solids at the meso- and nano-scales
- 2003
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Ingår i: Acta Materialia. - : Elsevier BV. - 1359-6454 .- 1873-2453. ; 51:19, s. 5675-5710
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Forskningsöversikt (refereegranskat)abstract
- Phase boundaries play an important role in setting the properties of multicomponent materials at both the meso- and nano-scales. In this review, we provide an overview of the modelling methods utilized in state-of-the-art research and engineering applications. We review the current physical understanding of how phase boundaries evolve, focusing on multicomponent systems. The recent advances in numerical modelling, fueled by powerful computers, have provided accurate and robust results that allow problems that are beyond the reach of analytic methods to be addressed. While the approaches used in engineering-oriented applications employ simplified microstructures, it is found that such models are quite useful in many problems. The ability to simulate realistic microstructures will further increase the power of materials modelling. We also highlight the differences between the sharp interface and diffuse interface approaches for modelling microstructural evolution. In addition, we identify future research topics in this area.
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