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- Björkman, Andrea, et al.
(författare)
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Aberrant recombination and repair during immunoglobulin class switching in BRCA1-deficient human B cells.
- 2015
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Ingår i: Proceedings of the National Academy of Sciences. - : Proceedings of the National Academy of Sciences. - 1091-6490 .- 0027-8424. ; 112:7, s. 2157-2162
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Tidskriftsartikel (refereegranskat)abstract
- Breast cancer type 1 susceptibility protein (BRCA1) has a multitude of functions that contribute to genome integrity and tumor suppression. Its participation in the repair of DNA double-strand breaks (DSBs) during homologous recombination (HR) is well recognized, whereas its involvement in the second major DSB repair pathway, nonhomologous end-joining (NHEJ), remains controversial. Here we have studied the role of BRCA1 in the repair of DSBs in switch (S) regions during immunoglobulin class switch recombination, a physiological, deletion/recombination process that relies on the classical NHEJ machinery. A shift to the use of microhomology-based, alternative end-joining (A-EJ) and increased frequencies of intra-S region deletions as well as insertions of inverted S sequences were observed at the recombination junctions amplified from BRCA1-deficient human B cells. Furthermore, increased use of long microhomologies was found at recombination junctions derived from E3 ubiquitin-protein ligase RNF168-deficient, Fanconi anemia group J protein (FACJ, BRIP1)-deficient, or DNA endonuclease RBBP8 (CtIP)-compromised cells, whereas an increased frequency of S-region inversions was observed in breast cancer type 2 susceptibility protein (BRCA2)-deficient cells. Thus, BRCA1, together with its interaction partners, seems to play an important role in repairing DSBs generated during class switch recombination by promoting the classical NHEJ pathway. This may not only provide a general mechanism underlying BRCA1's function in maintaining genome stability and tumor suppression but may also point to a previously unrecognized role of BRCA1 in B-cell lymphomagenesis.
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| 2. |
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| 3. |
- Qvist, Per Olov, 1948-
(författare)
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Jorden är vår arvedel : Landsbygden i svensk spelfilm 1940-1959
- 1986
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This study portrays one of the most important genres in Swedish film history,yet relatively unknown to today's film historians. The films about the countryside and the life,people and traditions of that particular milieu reached a peak in popularity in the years immediately after WW II followed by a somewhat small renaissance in the middle of the 1950's.But the genre is as old as the history of Swedish film itself and its roots can be traced back in Swedish literature and pictorial arts as shown in chapter three,Facts are given about the general organization of Swedish film prodduction as well as about the films covered by this study.The second half of the study is a thematic analysis of the films.This is done with reference to a long and persistent ideological tradition,"agrarian romanticism",which can be traced back to the end of the 19th century.In this the peasant is regarded as the most noble man and the very core of the society,a view that is expressed by most films in this genre in various ways.The fundamental themes include the following:the importance of preserving the inherited earth within the family and the peasant class thus safeguarding the society as a wholejthe stigmatization of various vagrant people as threats to this society;and finally the sharp distinction between the virtues of the countryside and the evils of the city.Finally there is a discussion of the importance of nature in these films,which is another important link back to the romantic tradition of the 19th century.The concluding chapter is a brief summary of the genre in the last decades,including its role in the field of contemporary television.
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