| 21. |
- Hedlund, Louise, et al.
(författare)
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Incubation and hatching conditions of laying hen chicks explain a large part of the stress effects from commercial large-scale hatcheries
- 2021
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Ingår i: Poultry Science. - : ELSEVIER. - 0032-5791 .- 1525-3171. ; 100:1
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Tidskriftsartikel (refereegranskat)abstract
- In commercial egg production, laying hen chicks are exposed to several stressful events during incubation, hatching, and their first hours in life. We have previously shown that hatching and processing are associated with increased corticosterone concentration and further affect behavior and stress sensitivity in a short- as well as long-term perspective. However, it is not known whether these long-term stress effects are caused by the hatchery processing (sex sorting, vaccination, conveying, and loading for transport) or if they are mainly caused by potentially stressful events before processing, during incubation and hatching. In the present study, the aim was to assess the effects of incubation and hatching only, compared to stress effects from the entire hatchery processing. We compared Lohmann LSL chicks incubated, hatched, and processed in a commercial hatchery with chicks incubated and hatched at the same time but not further processed. We studied behavior in a novel arena and during tonic immobility, as well as weight development and corticosterone reaction during a stress challenge. Processed chicks had poorer weight development and were more active in the novel arena test. However, there were no significant differences between the groups in corticosterone reactivity or tonic immobility. When comparing with previous data, both groups had elevated corticosterone concentrations compared to what we had previously reported from chicks hatched under calm and nonstressful conditions. In conclusion, incubation and hatching alone caused long-term stress effects in chickens, but further processing exacerbated these effects to some extent.
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| 22. |
- Hedlund, Louise, et al.
(författare)
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Stress during Commercial Hatchery Processing Induces Long-Time Negative Cognitive Judgement Bias in Chickens
- 2021
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Ingår i: Animals. - : MDPI. - 2076-2615. ; 11:4
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Tidskriftsartikel (refereegranskat)abstract
- Simple Summary Worldwide, billions of laying hen chicks are incubated, hatched and processed in industrial hatcheries every year. When exposed to stress, hormones are incorporated in the feathers of the birds. Here, we measured levels of the stress hormone corticosterone to investigate possible stress during the incubation. Further, animals can perceive their environment either in a positive (optimistic) or a negative (pessimistic) way. We investigated how the early hatchery experiences affects "optimism" and "pessimism". Commercially hatched chicks were exposed to a positive cue, an aversive cue, and ambiguous cues, in order to evaluate the cognitive welfare state of the animals. These chicks were compared to a group of non-stressed animals. Commercially incubated chicks did not have elevated levels of feather corticosterone, which implies that the main part of the stress effects from the hatchery originates from the period around hatch. Latencies to approach ambiguous cues were longer for the stressed chicks, i.e., these showed a more pessimistic-like behaviour. We conclude that the main part of the stress effects in commercially hatched chicks originates in the period around hatch, and further, that these birds show lasting levels of increased pessimism. This strongly indicates a long-time poorer welfare state for the animals. Worldwide, billions of laying hen chicks are incubated and processed under highly industrialised circumstances every year, which, as we have previously shown, has long-lasting effects. Here, we measured corticosterone incorporated in down feathers to investigate possible stress during the incubation and showed that commercially incubated chicks did not have elevated levels of feather corticosterone, which implies that the main part of the stress effects from hatchery originates from the perinatal period and the handling immediately post-hatch. Further, we investigated how the early hatchery stress affects the chicks cognitive welfare state, i.e., "optimism" and "pessimism". We exposed commercially hatched chickens to a positive cue, an aversive cue and ambiguous cues. The birds were tested at 1 and 10 w of age and the behaviour was compared with that of non-stressed chicks. Latencies to approach ambiguous cues were longer for the stressed chicks, both at 1 (p = 0.008) and at 10 (p = 0.020) weeks of age, i.e., these showed a more pessimistic-like behaviour. We conclude that the main part of the stress effects in commercially hatched chicks originates in the perinatal period, and further, that these birds show lasting levels of increased pessimism. This strongly indicates a long-time poorer welfare state for the animals.
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| 23. |
- Reinikainen, Marko, et al.
(författare)
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Resurrected Ceriodaphnia quadrangula highlight differences between pheno- and genotypic expressions
- 2012
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Ingår i: Ecology and Evolution. - : Wiley. - 2045-7758. ; 2:12, s. 2989-2998
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Tidskriftsartikel (refereegranskat)abstract
- The hatching of cladoceran ephippia from a 15-cm long sediment core was investigated, and Ceriodaphnia quadrangula clones were isolated from different sediment layers. Bosmina microfossil data were also analyzed, and compared with the corresponding data from a Pb210 dated core, which allowed us to infer the age of the sediment layers. Using changes in Bosmina microfossil morphologies, we were, furthermore, able to infer the presence of different regimes of fish predation. C. quadrangula was found to hatch in layers with an inferred age of approximately a century. Newly hatched individuals had smaller eye-size in sediment layers corresponding to high predation by young-of-the-year perch. Newly hatched individuals also generally had a marked neck-spine. In contrast, morphological characters of C. quadrangula clones reared in the laboratory over several generations showed no variation in relation to predation regime, indicating the absence of fixed genotype level changes. Furthermore, the laboratory grown clones only rarely produced a neck-spine. The results suggest phenotypic variation in response to the regime under which ephippia were produced.
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| 24. |
- Reinikainen, Marko, et al.
(författare)
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Resurrected past-century Ceriodaphnia quadrangula highlight differences between pheno- and genotypic expressions
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- The hatching of cladoceran ephippia from a 15 cm long sediment core was investigated, and Ceriodaphnia quadrangula -clones were isolated from different sediment layers. Bosmina-microfossil data were also analyzed, and compared with the corresponding data from a Pb210-dated core, which allowed us to infer the age of the sediment-layers. Using changes in Bosmina-microfossil morphologies, we were furthermore able to infer the presence of different regimes of fish-predation. C. quadrangula was found to hatch in layers with an inferred age of approximately a century. Newly hatched individuals had smaller eye-size in sediment layers corresponding to high predation by young-of-the-year perch. Newly hatched individuals also generally had a marked neck-spine. In contrast, morphological characters of C. quadrangula -clones reared in the laboratory over several generations showed no variation in relation to predation regime, indicating the absence of fixed genotype-level changes. Furthermore, the laboratory-grown clones only rarely produced a neck-spine. The results suggest phenotypic variation in response to the regime under which ephippia were produced.
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| 25. |
- Westerberg, Håkan
(författare)
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Spawning by the European eel across 2000 km of the Sargasso Sea
- 2019
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Ingår i: Biology Letters. - : The Royal Society. - 1744-9561 .- 1744-957X. ; 15
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Tidskriftsartikel (refereegranskat)abstract
- It has been known for about a century that European eels have a unique life history that includes offshore spawning in the Sargasso Sea about 5000-7000 km away from their juvenile and adult habitats in Europe and northern Africa. Recently hatched eel larvae were historically collected during Danish, German and American surveys in specific areas in the southern Sargasso Sea. During a 31 day period of March and April 2014, Danish and German research ships sampled for European eel larvae along 15 alternating transects of stations across the Sargasso Sea. The collection of recently hatched eel larvae (<= 12 mm) from 70 degrees W and eastward to 50 degrees W showed that the European eel had been spawning across a 2000 km wide region of the North Atlantic Ocean. Historical collections made from 1921 to 2007 showed that small larvae had also previously been collected in this wide longitudinal zone, showing that the spatial extent of spawning has not diminished in recent decades, irrespective of the dramatic decline in recruitment. The use of such a wide spawning area may be related to variations in the onset of the silver eel spawning migration, individual differences in their long-term swimming ability, or aspects of larval drift.
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| 26. |
- Broman, Elias, et al.
(författare)
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Oxygenation of anoxic sediments triggers hatching of zooplankton eggs
- 2015
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Ingår i: Proceedings of the Royal Society of London. Biological Sciences. - : The Royal Society. - 0962-8452 .- 1471-2954. ; 282:1817
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Tidskriftsartikel (refereegranskat)abstract
- Many coastal marine systems have extensive areas with anoxic sediments and it is not well known how these conditions affect the benthic-pelagic coupling. Zooplankton lay their eggs in the pelagic zone, and some sink and lie dormant in the sediment, before hatched zooplankton return to the water column. In this study, we investigated how oxygenation of long-term anoxic sediments affects the hatching frequency of dormant zooplankton eggs. Anoxic sediments from the brackish Baltic Sea were sampled and incubated for 26 days with constant aeration whereby, the sediment surface and the overlying water were turned oxic. Newly hatched rotifers and copepod nauplii (juveniles) were observed after 5 and 8 days, respectively. Approximately 1.5 × 105 nauplii per m-2 emerged from sediment turned oxic compared to 0.02 × 105 m-2 from controls maintained anoxic. This study demonstrated that re-oxygenation of anoxic sediments activated a large pool of buried zooplankton eggs, strengthening the benthic-pelagic coupling of the system. Modelling of the studied anoxic zone suggested that a substantial part of the pelagic copepod population can derive from hatching of dormant eggs. We suggest that this process should be included in future studies to understand population dynamics and carbon flows in marine pelagic systems.
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| 27. |
- Sniegula, Szymon, et al.
(författare)
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Size-mediated priority and temperature effects on intra-cohort competition and cannibalism in a damselfly
- 2019
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Ingår i: Journal of Animal Ecology. - : WILEY. - 0021-8790 .- 1365-2656. ; 88:4, s. 637-648
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Tidskriftsartikel (refereegranskat)abstract
- A shift in the relative arrival of offspring, for example a shift in hatching time, can affect competition at the intraspecific level through size-mediated priority effects, where the larger individuals gain more resources. These priority effects are likely to be affected by climate warming and the rate of intraspecific predation, that is cannibalism. In a laboratory experiment, we examined size-mediated priority effects in larvae of the univoltine damselfly, Lestes sponsa, at two different temperatures (21 and 23 degrees C). We created three size groups of larvae by manipulating hatching time: early hatched with a large size (extra-advanced), intermediate hatched with an intermediate size (advanced) and late hatched with a small size (non-advanced). Thereafter, we reared the larvae from these groups in non-mixed and mixed groups of 12 larvae. We found strong priority and temperature effects. First, extra-advanced larvae most often had higher survival, growth and development rates than non-advanced larvae in mixed groups, compared to groups that consisted of only extra-advanced larvae. Second, temperature increased growth and development rates and cannibalism. However, the strength of priority effects did not differ between the two experimental temperatures, because there was no statistical interaction between temperature and treatments. That is, the mixed and non-mixed groups of non-advanced, advanced and extra-advanced larvae showed the same relative change in life-history traits across the two temperatures. Non-advanced and advanced larvae had similar or higher growth rate and mass in mixed groups compared to non-mixed groups, suggesting that predation from advanced larvae in the mixed group released resources for the non-advanced and advanced larvae that survived despite cannibalism risk. Thus, a thinning effect occurred due to cannibalism caused by priority effects. The results suggest that a shift in the relative arrival of offspring can cause temperature-dependent priority effects, mediated through cannibalism, growth and development, which may change the size distribution and abundance of emerging aquatic insects.
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| 28. |
- Guo, Haoyu, et al.
(författare)
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Evaluation of starvation status in the early developmental stages of black rockfish (Sebastes schlegelii) based on morphological and histological characteristics
- 2023
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Ingår i: Journal of Fish Biology. - 0022-1112 .- 1095-8649.
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Tidskriftsartikel (refereegranskat)abstract
- Assessing the nutritional status and identifying major causes of mortality in larvae experiencing varying degrees of starvation are crucial for establishing appropriate feeding protocols and enhancing the welfare of hatchery-reared fish. The black rockfish Sebastes schlegelii is an important species in aquaculture and stock enhancement efforts in China, Japan, and Korea. This study aimed to identify optimal diagnostic morphometric indicators of starvation in newly hatched (0–6 days post-hatch, DPH) and postlarval stages (27–37 DPH) of this valuable fish species through histological analyses. Our findings revealed that certain morphometric parameters, including body length, the ratios of eye diameter to head height, body height to body length, and abdomen height to body height, exhibit sensitivity to starvation during both larval and postlarval stages. Particularly, the ratios of body height to body length and abdomen height to body height emerged as the most sensitive morphometric indicators of starvation. Histological examinations of the digestive system revealed rapid alterations in the morphology of hepatic parenchymal cells, accompanied by a significant decrease in the number of lipid cells in the liver during episodes of food deprivation. Starvation induced cellular degeneration in the digestive organs, manifested by reduced heights of epithelial cells and mucosal layers in the intestine, oesophagus, and stomach, along with degeneration and separation of muscle fibers. Among these variables, the height of the intestinal submucosa and muscle layer emerged as the most sensitive indicators reflecting nutritional conditions in newly hatched larvae. In contrast, the height of intestinal striated borders and mucosal folds proved to be the most sensitive indicators in the postlarval stage. Furthermore, the height of intestinal epithelial cells and the number of lipid vacuoles in enterocytes exhibited high sensitivity to food deprivation in both newly hatched larvae and postlarvae. These findings underscore the varying resilience of fish to starvation during different developmental phases and highlight the utility of morphological sensitivity characteristics as reliable diagnostic indices for assessing nutritional status in relation to starvation or suboptimal feeding during the early developmental stages of black rockfish in hatchery-reared processes.
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| 29. |
- Gabrielle, Lunden, et al.
(författare)
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Play ontogeny in young chickens is affected by domestication and early stress
- 2022
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Ingår i: Scientific Reports. - : NATURE PORTFOLIO. - 2045-2322. ; 12:1
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Tidskriftsartikel (refereegranskat)abstract
- Play is common in young homeotherm animals and has an important role as a tentative indicator of positive states of welfare. Furthermore, during domestication play is believed to have increased in frequency in several species as part of the domestication syndrome. Here, we studied the ontogeny of play in chickens in two experiments. The first compared the behavioural development between domesticated White Leghorn (WL) laying hen chicks and ancestral Red Junglefowl (RJF) and the second compared the same between WL chicks that had experienced the stress of commercial hatchery routines and a control group, hatched under calm conditions. In both experiments, 10 groups of four chicks each from each of the groups were moved twice per week to an enriched and fully enclosed play arena, starting at day 8 and finishing day 39 or 53 after hatch. In the arena, the frequency of play behaviours was recorded during 30 min and divided into object, locomotory and social play. In experiment one, total play as well as object play was significantly more common in WL whereas locomotor and social play was more common in RJF. In experiment two, total play was significantly more frequent in commercially hatched chicks, despite that none of the sub-categories differed significantly between the groups. In conclusion, domestication as well as early stress does affect the occurrence of play in chickens, but the effects are complex and require further research.
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| 30. |
- Gyllström, Mikael, et al.
(författare)
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Bioturbation as Driver of Zooplankton Recruitment, Biodiversity and Community Composition in Aquatic Ecosystems
- 2008
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Ingår i: Ecosystems. - : Springer Science and Business Media LLC. - 1432-9840 .- 1435-0629. ; 11:7, s. 1120-1132
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Tidskriftsartikel (refereegranskat)abstract
- In an experimental study we assessed if benthic bioturbating invertebrates affect the recruitment (hatching) of zooplankton from the sediment, and if this effect persists as differences in the zooplankton community in the water column, that is, if bioturbation quantitatively stimulates benthic-pelagic coupling. We investigated the effects of four different benthic invertebrates (Asellus aquaticus, Chironomus plumosus, Tubifex tubifex in the presence or absence of the predator Sialis lutaria). In total, 45 zooplankton taxa hatched from the sediment and the hatching success of some of these was dependent on the species identity of the bioturbating invertebrate. The predator Sialis reduced the abundance of all three invertebrate species, but tended to positively influence the zooplankton recruitment rates, possibly through increasing the activity of the bioturbating invertebrates. The most striking effect of bioturbation on the hatching and pelagic zooplankton community properties was that, on average, 11% more species hatched in the Asellus treatment than in any other treatment. This was also mirrored in the zooplankton water column community where, on average, 7% more species established a viable population in treatments with Asellus as bioturbator. In a complementary field survey, Asellus was more common in littoral than in profundal sediments. Because Asellus strongly affected recruitment of zooplankton in our experiment, we argue that bioturbation may partly explain why recruitment of resting stages of both phyto- and zooplankton is generally higher in littoral than in profundal areas.
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