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Sökning: L773:0250 7005 OR L773:1791 7530

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578.
  • Ruuth, Kristina, et al. (författare)
  • Differential resistance of melanoma cells to treatment with recombinant IFN-alpha2b and leukocyte IFN.
  • 2007
  • Ingår i: Anticancer Res. - 0250-7005. ; 27:4B, s. 2109-14
  • Tidskriftsartikel (refereegranskat)abstract
    • BACKGROUND: Interferon-alpha (IFN-alpha) subtypes bind to the same receptor and are expected to have the same biological functions. Whether or not leukocyte IFN, containing six major IFN-alpha proteins had the same anti-tumor effect as one subtype, recombinant IFN-alpha2b, was investigated. MATERIALS AND METHODS: Three melanoma lines were treated with both types of IFN, and the effect on proliferation and survival was estimated both after short-term and prolonged treatment. RESULTS: All the melanoma cell lines were sensitive to the antiproliferative effects of both IFN species during short-term treatment. However, upon prolonged culture, the frequency of resistant colony formation was significantly higher in cultures treated with IFN-alpha2b compared to those treated with leukocyte IFN. There was a qualitative difference between the resistant colonies selected by the two IFN species with respect to morphology, growth rate and sensitivity to apoptosis. CONCLUSION: The development of resistant clones occurred at a lower rate during long-term treatment with leukocyte IFN containing six major subtypes of IFN-alpha as compared to IFN-alpha2b.
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579.
  • Rydén, Lisa, et al. (författare)
  • Assessment of microvessel density in core needle biopsy specimen in breast cancer
  • 2004
  • Ingår i: Anticancer research. - 1791-7530. ; 24:1, s. 371-375
  • Tidskriftsartikel (refereegranskat)abstract
    • AIM: Estimation of microvessel density (MVD) in primary breast cancer in core needle biopsies (CNB) may predict response to systemic therapy. The aim of the present study was to explore the accuracy of assessment of MVD in CNB related to MVD in excised tumours. MATERIAL AND METHODS: MVD was estimated in core biopsies and subsequently excised tumours in 54 consecutive patients with breast cancer without pre-operative treatment. RESULTS: The correlation between MVD in CNB and excised tumours was non-significant. However, in tumours larger than 20 mm (r=0.56, p=0.005) and in lobular carcinomas (r=0.55, p=0.014) a significant correlation was observed. CONCLUSION: The overall accuracy between estimation of MVD on CNB and excised breast tumours was non-significant. The usefulness of MVD in CNB as a marker of response to systemic therapy should be further validated before it can be used in clinical practice.
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580.
  • Rydén, Lisa, et al. (författare)
  • Decreased angiogenic activity in breast cancer in ever-users of oral contraceptive therapy--preliminary report
  • 2003
  • Ingår i: Anticancer research. - 1791-7530. ; 23:3C, s. 2875-2878
  • Tidskriftsartikel (refereegranskat)abstract
    • BACKGROUND: Angiogenic activity defined by microvessel density or measurement of vascular endothelial growth factor is a key process under physiological and malignant conditions in steroid hormone responding organs. The aim of this study was to relate microvessel density (MVD) in primary breast cancer to reproductive data and use of exogenous hormones. MATERIAL AND METHODS: MVD was calculated retrospectively in forty-two consecutive tumours and related to clinical, histopathological and gynecological data. RESULTS: Tumours in ever-users of oral contraceptive therapy (OC) had lower MVD (p = 0.002), a finding not explained by smaller tumour size or lower histological grade. There was no influence on MVD by other reproductive data. CONCLUSION: These preliminary data on a supposed interaction between the use of OC and angiogenesis in breast cancer indicate that biological properties in breast tumours may be altered by ever-use of OC, but have to be further explored in an extended number of patients.
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