| 571. |
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| 572. |
- Bernado, P, et al.
(författare)
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Combined use of NMR relaxation measurements and hydrodynamic calculations to study protein association. Evidence for tetramers of low molecular weight protein tyrosine phosphatase in solution
- 2003
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 125:4, s. 916-923
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Tidskriftsartikel (refereegranskat)abstract
- We describe a novel method for determining weak association constants of oligomeric protein complexes formed transiently under equilibrium conditions. This type of equilibrium process is recognized as being biologically important, but generally hard to study. Heteronuclear spin relaxation rates measured at multiple protein concentrations are analyzed using relaxation rates predicted from hydrodynamic calculations, yielding equilibrium constants and structural characterization of the protein complexes. The method was used to study the oligomerization equilibrium of bovine low molecular weight protein tyrosine phosphatase. X-ray structures of monomeric and dimeric forms of the protein have been reported previously. Using longitudinal and transverse 15N relaxation rates measured at four different protein concentrations, we detected the monomer, dimer, and a previously unknown tetramer and measured the dissociation constants of the equilibria involving these species. A comparison of experimental and predicted relaxation rates for individual backbone amide 15N spins enabled delineation of the tetramerization interface. The results suggest a novel concept for substrate modulation of enzymatic activity based on a "supramolecular proenzyme". The fast and reversible switching of the "supramolecular proenzyme" would have obvious advantages for the regulation of enzymes involved in cell signaling pathways.
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| 573. |
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| 574. |
- de La Torre, Amanda R, et al.
(författare)
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Insights into conifer giga-genomes
- 2014
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Ingår i: Plant Physiology. - : American Society of Plant Biologists. - 0032-0889 .- 1532-2548. ; 166:4, s. 1724-1732
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Tidskriftsartikel (refereegranskat)abstract
- Insights from sequenced genomes of major land plant lineages have advanced research in almost every aspect of plant biology. Until recently, however, assembled genome sequences of gymnosperms have been missing from this picture. Conifers of the pine family (Pinaceae) are a group of gymnosperms that dominate large parts of the world's forests. Despite their ecological and economic importance, conifers seemed long out of reach for complete genome sequencing, due in part to their enormous genome size (20-30 Gb) and the highly repetitive nature of their genomes. Technological advances in genome sequencing and assembly enabled the recent publication of three conifer genomes: white spruce (Picea glauca), Norway spruce (Picea abies), and loblolly pine (Pinus taeda). These genome sequences revealed distinctive features compared with other plant genomes and may represent a window into the past of seed plant genomes. This Update highlights recent advances, remaining challenges, and opportunities in light of the publication of the first conifer and gymnosperm genomes.
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| 575. |
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| 576. |
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| 577. |
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| 578. |
- De La Torre Luque, Pedro, et al.
(författare)
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FLUKA cross sections for cosmic-ray interactions with the DRAGON2 code
- 2022
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Ingår i: Journal of Cosmology and Astroparticle Physics. - : IOP Publishing. - 1475-7516. ; 2022:7
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Tidskriftsartikel (refereegranskat)abstract
- Secondary particles produced in spallation reactions of cosmic rays with the interstellar gas provide valuable information that allow us to investigate the injection and transport of charged particles in the Galaxy. A good understanding of the cross sections of production of these particles is crucial to correctly interpret our models, although the existing experimental data is very scarce and uncertain. We have developed a new set of cross sections, both inelastic and inclusive, computed with the FLUKA Monte Carlo nuclear code and tested its compatibility with CR data. Inelastic and inclusive cross sections have been compared to the most up-to-date data and parameterisations finding a general good agreement. Then, these cross sections have been implemented in the DRAGON2 code to characterize the spectra of CR nuclei up to Z = 26 and the secondary-to-primary ratios of B, Be and Li. Interestingly, we find that the FLUKA cross sections allow us to predict an energy-dependence of the B, Be and Li flux ratios which is compatible with AMS-02 data and to reproduce simultaneously these flux ratios with a scaling lower than 20%. Finally, we implement the cross sections of production of gamma rays, calculated with FLUKA, in the Gammasky code and compute diffuse gamma-ray sky maps and the local HI emissivity spectrum, finding a very good agreement with Fermi Large Area Telescope data.
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| 579. |
- De La Torre Luque, Pedro, et al.
(författare)
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The FLUKA cross sections for cosmic-ray leptons and uncertainties on current positron predictions
- 2023
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Ingår i: Journal of Cosmology and Astroparticle Physics. - 1475-7516. ; :10
-
Tidskriftsartikel (refereegranskat)abstract
- Cosmic-ray (CR) antiparticles have the potential to reveal signatures of unexpected astrophysical processes and even new physics beyond the Standard Model. Recent CR detectors have provided accurate measurements of the positron flux, revealing the so-called positron excess at high energies. However, the uncertainties related to the modelling of the local positron flux are still very high, significantly affecting our models of positron emission from pulsars and current dark matter searches. In this work, we report a new set of cross sections for positron and electron production derived from the FLUKA code. We compare them with the most extended cross-section data-sets and show the impact of neglecting the positron production from heavy CRs. Then, we review the most significant sources of uncertainties in our current estimations of the secondary positron flux at Earth and examine for the first time the impact of considering the spiral arm structure of the Galaxy in these estimations. Finally, we provide state-of-the-art predictions of the local positron flux and discuss the limitations of our dark matter searches with positrons and difficulties to determine the contribution from pulsars to the positron flux at low energies.
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| 580. |
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