| 61. |
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| 62. |
- Alonso-Sáez, Laura, et al.
(författare)
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Role for urea in nitrification by polar marine Archaea
- 2012
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 109:44, s. 17989-17994
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Tidskriftsartikel (refereegranskat)abstract
- Despite the high abundance of Archaea in the global ocean, their metabolism and biogeochemical roles remain largely unresolved. We investigated the population dynamics and metabolic activity of Thaumarchaeota in polar environments, where these microorganisms are particularly abundant and exhibit seasonal growth. Thaumarchaeota were more abundant in deep Arctic and Antarctic waters and grew throughout the winter at surface and deeper Arctic halocline waters. However, in situ single-cell activity measurements revealed a low activity of this group in the uptake of both leucine and bicarbonate (<5% Thaumarchaeota cells active), which is inconsistent with known heterotrophic and autotrophic thaumarchaeal lifestyles. These results suggested the existence of alternative sources of carbon and energy. Our analysis of an environmental metagenome from the Arctic winter revealed that Thaumarchaeota had pathways for ammonia oxidation and, unexpectedly, an abundance of genes involved in urea transport and degradation. Quantitative PCR analysis confirmed that most polar Thaumarchaeota had the potential to oxidize ammonia, and a large fraction of them had urease genes, enabling the use of urea to fuel nitrification. Thaumarchaeota from Arctic deep waters had a higher abundance of urease genes than those near the surface suggesting genetic differences between closely related archaeal populations. In situ measurements of urea uptake and concentration in Arctic waters showed that small-sized prokaryotes incorporated the carbon from urea, and the availability of urea was often higher than that of ammonium. Therefore, the degradation of urea may be a relevant pathway for Thaumarchaeota and other microorganisms exposed to the low-energy conditions of dark polar waters.
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| 63. |
- Björn, Lars Olof, et al.
(författare)
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Technical discussion I - Underwater light measurement and light absorption by algae
- 1996
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Ingår i: Scientia Marina. - 0214-8358. ; 60:Suppl.1, s. 293-297
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Tidskriftsartikel (refereegranskat)abstract
- In this paper concepts and nomenclature of light measurements are discussed. The particular problems of underwater light measurements and the calibration of the equipment were presented. The pros and cons with spectroradiometers and broad band sensors were discussed. The use of specific ultraviolet-B sources for algal photobiology is recommended.
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| 64. |
- Buggert, Marcus, et al.
(författare)
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Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease
- 2018
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Ingår i: PLoS Pathogens. - : Public Library of Science (PLoS). - 1553-7374. ; 14:4
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Tidskriftsartikel (refereegranskat)abstract
- CD4+ T cells subsets have a wide range of important helper and regulatory functions in the immune system. Several studies have specifically suggested that circulating effector CD4+ T cells may play a direct role in control of HIV replication through cytolytic activity or autocrine β-chemokine production. However, it remains unclear whether effector CD4+ T cells expressing cytolytic molecules and β-chemokines are present within lymph nodes (LNs), a major site of HIV replication. Here, we report that expression of β-chemokines and cytolytic molecules are enriched within a CD4+ T cell population with high levels of the T-box transcription factors T-bet and eomesodermin (Eomes). This effector population is predominately found in peripheral blood and is limited in LNs regardless of HIV infection or treatment status. As a result, CD4+ T cells generally lack effector functions in LNs, including cytolytic capacity and IFNγ and β-chemokine expression, even in HIV elite controllers and during acute/early HIV infection. While we do find the presence of degranulating CD4+ T cells in LNs, these cells do not bear functional or transcriptional effector T cell properties and are inherently poor to form stable immunological synapses compared to their peripheral blood counterparts. We demonstrate that CD4+ T cell cytolytic function, phenotype, and programming in the peripheral blood is dissociated from those characteristics found in lymphoid tissues. Together, these data challenge our current models based on blood and suggest spatially and temporally dissociated mechanisms of viral control in lymphoid tissues.
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| 65. |
- Ershova, Victoria B., et al.
(författare)
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The De Long Islands : A missing link in unraveling the Paleozoic paleogeography of the Arctic
- 2016
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Ingår i: Gondwana Research. - : Elsevier BV. - 1342-937X .- 1878-0571. ; 35, s. 305-322
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Tidskriftsartikel (refereegranskat)abstract
- The vast Laptev and East Siberian shelves in the eastern Russian Arctic, largely covered by a shallow sea and buried beneath sea ice for 9 months of the year, remain one of the least studied parts of continental crust of the Earth and represent a big unknown when performing pre-Cenozoic geodynamic reconstructions of the Arctic. The De Long islands provide a vitally important window into the geology of this area and are a key for [1] understanding the Early Paleozoic history of the Amerasian Arctic. Four of them (Jeanette, Henrietta, Bennett and Zhokhov islands) were studied using structural data, petrographic and geochemical analyses and U-Pb zircon age dating to offer the following new constraints for the Early Paleozoic paleogeography of the Arctic realm. The basement beneath the De Long Islands is of Late Neoproterozoic to earliest Cambrian age, about 670-535 Ma. In the Early Paleozoic, the De Long Islands were located along the broad Timanian margin of Baltica, with a clastic sediment provenance from the Timanian, Grenville-Sveconorwegian, and Baltic Shield domains. The Cambro-Ordovician volcaniclastic successions on Jeannette and Henrietta islands formed part of a continental margin volcanic arc with a corresponding back-arc basin located to the south (in present co-ordinates). On the continent-ward side of the back-arc basin, shallow marine shelf clastic and carbonate rocks were deposited, which are exposed today on Bennett Island in the south-west of the archipelago (in modern coordinates). The De Long Islands together with other continental blocks, such as Severnaya Zemlya, Arctic Alaska-Chukotka, and the Alexander Terrane, formed the contiguous active continental margin of Baltica during the Early Paleozoic. Today however, these terranes are spread out over a distance of 5000 km across the Arctic and eastern Pacific margins due to the subsequent opening of a series of Late Paleozoic, Mesozoic and Cenozoic oceanic basins.
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| 66. |
- Estrada, EJ, et al.
(författare)
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Combined treatment of intrapancreatic autologous bone marrow stem cells and hyperbaric oxygen in type 2 diabetes mellitus
- 2008
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Ingår i: Cell transplantation. - : SAGE Publications. - 0963-6897 .- 1555-3892. ; 17:12, s. 1295-1304
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Tidskriftsartikel (refereegranskat)abstract
- The objective of this study was to determine whether the combination therapy of intrapancreatic autologous stem cell infusion (ASC) and hyperbaric oxygen treatment (HBO) before and after ASC can improve islet function and metabolic control in patients with type 2 diabetes mellitus (T2DM). This prospective phase 1 study enrolled 25 patients with T2DM who received a combination therapy of intrapancreatic ASC and periinfusion HBO between March 2004 and October 2006 at Stem Cells Argentina Medical Center Buenos Aires, Argentina. Clinical variables (body mass index, oral hypoglycemic drugs, insulin requirement) and metabolic variables (fasting plasma glucose, C-peptide, HbA1c, and calculation of C-peptide/glucose ratio) were assessed over quartile periods starting at baseline and up to 1 year follow-up after intervention. Means were calculated in each quartile period and compared to baseline. Seventeen male and eight female patients were enrolled. Baseline variables expressed as means ± SEs were: age 55 ± 2.14 years, diabetes duration 13.2 ± 1.62 years, insulin dose 34.8 ± 2.96 U/day, and BMI 27.11 ± 0.51. All metabolic variables showed significant improvement when comparing baseline to 12 months follow-up, respectively: fasting glucose 205.6 ± 5.9 versus 105.2 ± 14.2 mg/dl, HbA1c 8.8 ± 0.2 versus 6.0 ± 0.4%, fasting C-peptide 1.5 ± 0.2 versus 3.3 ± 0.3 ng/ml, C-peptide/glucose ratio 0.7 ± 0.2 versus 3.5 ± 0.3, and insulin requirements 34.8 ± 2.9 versus 2.5 ± 6.7 U/day. BMI remained constant over the 1-year follow-up. Combined therapy of intrapancreatic ASC infusion and HBO can improve metabolic control and reduce insulin requirements in patients with T2DM. Further randomized controlled clinical trials will be required to confirm these findings.
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| 67. |
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| 68. |
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| 69. |
- Kastlander, Johan, et al.
(författare)
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Development of methods to use CdTe detectors in field measurements
- 2006
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Ingår i: Journal of Physics, Conference Series. - : IOP Publishing. - 1742-6588 .- 1742-6596. ; 41, s. 523-526
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Tidskriftsartikel (refereegranskat)abstract
- We are currently investigating the possibility to use CdTe detectors for in situ determinations of radionuclide concentration in soil. Buried activity can be reliably determined by a comparison of the count rate in the photo peak and the region between the photo peak and Compton edge. However, the pulse-height spectrum from CdTe detectors is severely deteriorated, due to poor charge collection, in particular for high gamma-ray energies. Our efforts have, therefore, been concentrated on improving the peak to valley ratio for such detectors. A simple, non-discriminating, algorithm for the analysis of output from two amplifiers with different shaping times is described. By means of this algorithm the peak-tovalley ratio for a small planar CdTe detector is improved by more than a factor of six compared to the uncorrected ratio without loss of efficiency.
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| 70. |
- Kemp, John P, et al.
(författare)
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Phenotypic dissection of bone mineral density reveals skeletal site specificity and facilitates the identification of novel loci in the genetic regulation of bone mass attainment.
- 2014
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Ingår i: PLoS genetics. - : Public Library of Science (PLoS). - 1553-7404. ; 10:6
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Tidskriftsartikel (refereegranskat)abstract
- Heritability of bone mineral density (BMD) varies across skeletal sites, reflecting different relative contributions of genetic and environmental influences. To quantify the degree to which common genetic variants tag and environmental factors influence BMD, at different sites, we estimated the genetic (rg) and residual (re) correlations between BMD measured at the upper limbs (UL-BMD), lower limbs (LL-BMD) and skull (SK-BMD), using total-body DXA scans of ∼ 4,890 participants recruited by the Avon Longitudinal Study of Parents and their Children (ALSPAC). Point estimates of rg indicated that appendicular sites have a greater proportion of shared genetic architecture (LL-/UL-BMD rg = 0.78) between them, than with the skull (UL-/SK-BMD rg = 0.58 and LL-/SK-BMD rg = 0.43). Likewise, the residual correlation between BMD at appendicular sites (r(e) = 0.55) was higher than the residual correlation between SK-BMD and BMD at appendicular sites (r(e) = 0.20-0.24). To explore the basis for the observed differences in rg and re, genome-wide association meta-analyses were performed (n ∼ 9,395), combining data from ALSPAC and the Generation R Study identifying 15 independent signals from 13 loci associated at genome-wide significant level across different skeletal regions. Results suggested that previously identified BMD-associated variants may exert site-specific effects (i.e. differ in the strength of their association and magnitude of effect across different skeletal sites). In particular, variants at CPED1 exerted a larger influence on SK-BMD and UL-BMD when compared to LL-BMD (P = 2.01 × 10(-37)), whilst variants at WNT16 influenced UL-BMD to a greater degree when compared to SK- and LL-BMD (P = 2.31 × 10(-14)). In addition, we report a novel association between RIN3 (previously associated with Paget's disease) and LL-BMD (rs754388: β = 0.13, SE = 0.02, P = 1.4 × 10(-10)). Our results suggest that BMD at different skeletal sites is under a mixture of shared and specific genetic and environmental influences. Allowing for these differences by performing genome-wide association at different skeletal sites may help uncover new genetic influences on BMD.
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