| 11. |
- Gourdon, Pontus Emanuel, 1978, et al.
(författare)
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Optimized in vitro and in vivo expression of proteorhodopsin: A seven-transmembrane proton pump
- 2008
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Ingår i: Protein Expression and Purification. - : Elsevier BV. - 1096-0279 .- 1046-5928. ; 58:1, s. 103-113
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Tidskriftsartikel (refereegranskat)abstract
- Proteorhodopsin is an integral membrane light-harvesting proton pump that is found in bacteria distributed throughout global surface waters. Here, we present a protocol for functional in vitro production of pR using a commercial cell-free synthesis system yielding 1.0 mg purified protein per milliliter of cell lysate. We also present an optimized protocol for in vivo over-expression of pR in Escherichia coli, and a two-step purification yielding 5 mg of essentially pure functional protein per liter of culture. Both approaches are straightforward, rapid, and easily scalable. Thus either may facilitate the exploitation of pR for commercial biotechnological applications. Finally, the implications of some observations of the in vitro synthesis behavior, as well as preliminary results towards a structural determination of pR are discussed.
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| 12. |
- Hansson, Caroline, 1981, et al.
(författare)
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Risk factors for suicide in bipolar disorder: a cohort study of 12 850 patients
- 2018
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Ingår i: Acta Psychiatrica Scandinavica. - : Wiley. - 0001-690X .- 1600-0447. ; 138:5, s. 456-463
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Tidskriftsartikel (refereegranskat)abstract
- ObjectiveMethodBipolar disorder carries a high risk of suicide. Identification of risk factors is important. The aim of this study was to study risk factors for suicide in a large cohort of men and women with bipolar disorder. A prospective cohort study using clinical data from the Swedish National Quality Register for Bipolar Affective Disorder (BipolaR). The outcome variable was suicide captured in the Cause of Death Register between 2004 and 2014. Hazard ratios (HR) were calculated using Cox proportional hazards models. ResultsConclusionsOf 12 850 persons (4844 men and 8006 women) with bipolar disorder, 90 (55 men and 35 women) died by suicide during the follow-up period (between 1 and 10 years). Male sex (HR 2.56), living alone (HR 2.45), previous suicide attempts (HR 4.10), comorbid psychiatric disorder (HR 2.64), recent affective episodes (HR 2.39), criminal conviction (HR 4.43), psychiatric inpatient care (HR 2.79), and involuntary commitment (HR 3.50) were significant risk factors for suicide. Several of the statistically significant risk factors for suicide in bipolar disorder differed between men and women. Risk factors for suicide in bipolar disorder include factors associated with suicide in general, but also diagnosis-specific factors.
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| 13. |
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| 14. |
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| 15. |
- Hansson, Mikael, 1975-
(författare)
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Analys i arbetsrätt
- 2013
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Annan publikation (övrigt vetenskapligt/konstnärligt)
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| 16. |
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| 17. |
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| 18. |
- Hansson, Mattias, et al.
(författare)
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Artifactual insulin release from differentiated embryonic stem cells.
- 2004
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Ingår i: Diabetes. - 0012-1797. ; 53:10, s. 2603-9
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Tidskriftsartikel (refereegranskat)abstract
- Several recent reports claim the generation of insulin-producing cells from embryonic stem cells via the differentiation of progenitors that express nestin. Here, we investigate further the properties of these insulin-containing cells. We find that although differentiated cells contain immunoreactive insulin, they do not contain proinsulin-derived C-peptide. Furthermore, we find variable insulin release from these cells upon glucose addition, but C-peptide release is never detected. In addition, many of the insulin-immunoreactive cells are undergoing apoptosis or necrosis. We further show that cells cultured in the presence of a phosphoinositide 3-kinase inhibitor, which previously was reported to facilitate the differentiation of insulin(+) cells, are not C-peptide immunoreactive but take up fluorescein isothiocyanate-labeled insulin from the culture medium. Together, these data suggest that nestin(+) progenitor cells give rise to a population of cells that contain insulin, not as a result of biosynthesis but from the uptake of exogenous insulin. We conclude that C-peptide biosynthesis and secretion should be demonstrated to claim insulin production from embryonic stem cell progeny.
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| 19. |
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| 20. |
- Hansson, Mikael, 1986-
(författare)
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Combinatorics and topology related to involutions in Coxeter groups
- 2018
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This dissertation consists of three papers in combinatorial Coxeter group theory.A Coxeter group is a group W generated by a set S, where all relations can be derived from the relations s2 = e for all s ? S, and (ss′)m(s,s′) = e for some pairs of generators s ≠ s′ in S, where e ? W is the identity element and m(s, s′) is an integer satisfying that m(s, s′) = m(s′, s) ≥ 2. Two prominent examples of Coxeter groups are provided by the symmetric group Sn (i.e., the set of permutations of {1, 2, . . . , n}) and finite reflection groups (i.e., finite groups generated by reflections in some real euclidean space). There are also important infinite Coxeter groups, e.g., affine reflection groups.Every Coxeter group can be equipped with various natural partial orders, the most important of which is the Bruhat order. Any subset of a Coxeter group can then be viewed as an induced subposet.In Paper A, we study certain posets of this kind, namely, unions of conjugacy classes of involutions in the symmetric group. We obtain a complete classification of the posets that are pure (i.e., all maximal chains have the same length). In particular, we prove that the set of involutions with exactly one fixed point is pure, which settles a conjecture of Hultman in the affirmative. When the posets are pure, we give their rank functions. We also give a short, new proof of the EL-shellability of the set of fixed-point-free involutions, established by Can, Cherniavsky, and Twelbeck.Paper B also deals with involutions in Coxeter groups. Given an involutive automorphism θ of a Coxeter system (W, S), letℑ(θ) = {w ? W | θ(w) = w−1}be the set of twisted involutions. In particular, ℑ(id) is the set of ordinary involutions in W. It is known that twisted involutions can be represented by words in the alphabet = { | s ? S}, called -expressions. If ss′ has finite order m(s, s′), let a braid move be the replacement of ′ ⋯ by ′ ′ ⋯, both consisting of m(s, s′) letters. We prove a word property for ℑ(θ), for any Coxeter system (W, S) with any θ. More precisely, we provide a minimal set of moves, easily determined from the Coxeter graph of (W, S), that can be added to the braid moves in order to connect all reduced -expressions for any given w ? ℑ(θ). This improves upon a result of Hamaker, Marberg, and Pawlowski, and generalises similar statements valid in certain types due to Hu, Zhang, Wu, and Marberg.In Paper C, we investigate the topology of (the order complexes of) certain posets, called pircons. A special partial matching (SPM) on a poset is a matching of the Hasse diagram satisfying certain extra conditions. An SPM without fixed points is precisely a special matching as defined by Brenti. Let a pircon be a poset in which every non-trivial principal order ideal is finite and admits an SPM. Thus pircons generalise Marietti’s zircons. Our main result is that every open interval in a pircon is a PL ball or a PL sphere.An important subset of ℑ(θ) is the set ?(θ) = {θ(w−1)w | w ? W} of twisted identities. We prove that if θ does not flip any edges with odd labels in the Coxeter graph, then ?(θ), with the order induced by the Bruhat order on W, is a pircon. Hence, its open intervals are PL balls or spheres, which confirms a conjecture of Hultman. It is also demonstrated that Bruhat orders on Rains and Vazirani’s quasiparabolic W-sets (under a boundedness assumption) form pircons. In particular, this applies to all parabolic quotients of Coxeter groups.
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