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Sökning: WFRF:(Holst Olle)

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51.
  • Larsson, L, et al. (författare)
  • The measurement of cell growth in coal slurries
  • 1989
  • Ingår i: Biotechnology Techniques. - 0951-208X. ; 3:5, s. 295-298
  • Tidskriftsartikel (refereegranskat)abstract
    • Microbial growth in coal slurries can be detected if surfactant is present in the samples by analysing the protein concentration after disintegration of the cells. Without surfactant present in the samples, the proteins are readily adsorbed on the coal surfaces. A suitable surfactant is sodium dodecyl sulphate at 0.5 to 1% of the sample volume
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52.
  • Latifian, Maryam, et al. (författare)
  • Nitrogen and Phosphorus Removal from Urine by Sequential Struvite Formation and Recycling Process
  • 2014
  • Ingår i: Clean - Soil, Air, Water. - : Wiley. - 1863-0650. ; 42:8, s. 1157-1161
  • Tidskriftsartikel (refereegranskat)abstract
    • Recovering nutrients from urine as solid minerals such as struvite can eliminate many hurdles related to the use of liquid urine as fertilizer in the current urine separation systems. The focus of this study has been to increase nitrogen and phosphorus removal efficiencies by developing a struvite formation and recycling process. Struvite crystallization was carried out in urine samples by addition of MgO where 92% of phosphorus as orthophosphate was recovered. In order to optimize the use of calcined struvite in the struvite recycling step, four calcination treatments were tested and calcination at 200 degrees C for 3 h was chosen for further experiments. To remove the remaining ammonia present in urine a struvite recycling step was used subsequently. Through sequential struvite formation and struvite recycling process up to 90% of ammonium nitrogen was successfully removed.
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53.
  • Lindström, Cecilia, et al. (författare)
  • An oat bran-based beverage reduce postprandial glycaemia equivalent to yoghurt in healthy overweight subjects.
  • 2015
  • Ingår i: International Journal of Food Sciences and Nutrition. - : Informa UK Limited. - 1465-3478 .- 0963-7486. ; 66:6, s. 700-705
  • Tidskriftsartikel (refereegranskat)abstract
    • An acute meal study was performed to determine postprandial glucose and insulin responses after consumption of two fermented oat bran-based beverages (with and without exopolysaccharides) and yoghurt. This randomized, single-blind, within-subject study included 18 healthy, overweight participants. Four breakfast meals, including a reference meal, were tested; all meals contained 50 g of available carbohydrates, but differed in energy and macronutrient composition. All experimental meals reduced the postprandial glucose response compared with the reference meal. The oat drinks as well as the yoghurt elicited higher early (0-15 min) insulin responses, but the overall insulinaemia were similar to the reference meal. A new food product containing fermented liquid oat bran and milk reduced the postprandial blood glucose response as efficiently as yoghurt after a high-glycaemic index white wheat bread meal, but the presence of microbial exopolysaccharides did not affect the outcome.
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54.
  • Lindström, Cecilia, et al. (författare)
  • Effects of Pediococcus parvulus 2.6 and its exopolysaccharide on plasma cholesterol levels and inflammatory markers in mice
  • 2012
  • Ingår i: AMB Express. - : Springer Science and Business Media LLC. - 2191-0855. ; 2:66
  • Tidskriftsartikel (refereegranskat)abstract
    • Intake of dietary fibres may reduce the prevalence of physiological risk factors of the metabolic syndrome, such as high plasma lipid levels and low-grade inflammatory state. Dietary fibres are usually of plant origin however microbial exopolysaccharides (EPSs) have analogue structures that could potentially exert similar physiological effects. Pediococcus parvulus 2.6 (Pd 2.6) excretes a ropy EPS and has previously shown probiotic potential. The aim of this work was to evaluate physiological effects of Pd 2.6 and its EPS in vivo. The live Pd 2.6 (both the ropy and non-ropy isogenic variant) and its purified EPS were fed to hypercholesterolemic LDL-receptor deficient mice for 6 weeks to investigate their effects on cholesterol levels and the inflammatory tone of the animals. Both variants of Pd 2.6 survived passage through the mouse gut fulfilling an important criterion of probiotics. The ability to produce EPS was conferring an advantage to survival (faecal recovery of 3.7 (1.9-8.7) vs. 0.21 (0.14-0.34) *108 CFU, P < 0.001, median and 25th and 75th percentiles). The ropy Pd 2.6 decreased the levels of soluble vascular cell adhesion molecule-1 compared to the EPS alone (591 ± 14 vs. 646 ± 13 ng/ml, P < 0.05). An increase in liver weight in mice fed the purified EPS was observed, but with no change in liver lipids. No changes in blood lipids were detected in any group. Further the EPS induced growth of the caecal tissue and increased the amount of caecal content showing bulking properties like that of a dietary fibre.
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55.
  • Lindström, Cecilia, et al. (författare)
  • Evaluation of commercial microbial hydrocolloids concerning their effects on plasma lipids and caecal formation of SCFA in mice
  • 2012
  • Ingår i: Food Hydrocolloids. - : Elsevier BV. - 0268-005X. ; 28:2, s. 367-372
  • Tidskriftsartikel (refereegranskat)abstract
    • Exopolysaccharides (EPS) are excreted by microorganisms into the surrounding environment and have been shown to have various physiological effects and are commonly used as food additives due to their rheological properties. Four commercially available microbial EPS with different polymeric structures and composition were tested in LDL receptor knock-out mice to investigate their effect on blood cholesterol, lipoproteins and caecal formation of SCFA. After four weeks on a Western diet supplemented with 4% EPS there were significant increases in caecal content and caecal tissue weight for the EPS groups compared to the control. The total pool of caecal short chain fatty acids was increased when mice were fed scleroglucan, xanthan and dextran. There were no differences in plasma cholesterol levels on the experimental diets compared to the control. Plasma triglycerides did not differ between groups. The results indicate that EPS supplementation to a Western diet may help in maintaining a healthy intestinal environment. (C) 2012 Elsevier Ltd. All rights reserved.
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56.
  • Lindström, Cecilia, et al. (författare)
  • Oral administration of live exopolysaccharide-producing Pediococcus parvulus, but not purified exopolysaccharide suppressed Enterobacteriaceae without affecting bacterial diversity in caecum of mice.
  • 2013
  • Ingår i: Applied and Environmental Microbiology. - 0099-2240. ; 79:16, s. 5030-5037
  • Tidskriftsartikel (refereegranskat)abstract
    • Growing evidence indicates that the gut microbiota could have an important role in the development of diet and lifestyle induced diseases. It has been shown that modulation of the gut microbiota by means of probiotics and prebiotics could improve host health. An oat based product fermented by the exopolysaccharide (EPS)-producing Pediococcus parvulus 2.6 has been reported to have a bifidogenic effect. To find out whether the effect could be attributed to the EPS or the bacterium, mice were fed a diet supplemented with 2 % purified EPS or 10(8) CFU/g of live P. parvulus 2.6 for six weeks. Both supplementations altered the gut microbiota composition but in different directions. Purified EPS not only significantly lowered the microbial diversity (P < 0.001) but decreased the bifidobacterial population (P = 0.01). In contrast, the live EPS-producing P. parvulus 2.6 antagonized Enterobacteriaceae without disturbing the homeostasis of the caecal microbiota.
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57.
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58.
  • Martensson, O, et al. (författare)
  • Growth and exopolysaccharide formation by Pediococcus damnosus 2.6 in beta-glucan suspensions of oat and barley
  • 2005
  • Ingår i: LWT - Food Science and Technology. - : Elsevier BV. - 0023-6438. ; 38:2, s. 151-155
  • Tidskriftsartikel (refereegranskat)abstract
    • The formation of exopolysaccharides (EPS) by Pediococcus damnosus strain 2.6 was investigated in two different high P-glucan suspensions of oat (OBC) and barley (BBC). Bacterial growth was confirmed by measuring pH, colony forming units (cfu), viscosity and ropiness during growth. Growth and an increase in viscosity of the beta-glucan suspension based on oat were observed whereas only a minor growth and no significant effect on viscosity was seen in the beta-glucans suspension based on barley. Higher bacterial growth and higher final viscosity and ropiness were observed in samples that were mixed with the OBC and an ordinary commercial oat-base (Adavena(R) G40). These results show the potential of using a combination of a high beta-glucan suspension of cereals to generate fermented products containing both high levels of native beta-glucans from oats or barley and also microbial beta-glucans from the starter culture.
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