| 51. |
- Gunnarsson, Rebeqa, et al.
(författare)
-
Screening for copy-number alterations and loss of heterozygosity in chronic lymphocytic leukemia-A comparative study of four differently designed, high resolution microarray platforms
- 2008
-
Ingår i: Genes, Chromosomes and Cancer. - : Wiley. - 1045-2257 .- 1098-2264. ; 93, s. 0536-0536
-
Tidskriftsartikel (refereegranskat)abstract
- Screening for gene copy-number alterations (CNAs) has improved by applying genome-wide microarrays, where SNP arrays also allow analysis of loss of heterozygozity (LOH). We here analyzed 10 chronic lymphocytic leukemia (CLL) samples using four different high-resolution platforms: BAC arrays (32K), oligonucleotide arrays (185K, Agilent), and two SNP arrays (250K, Affymetrix and 317K, Illumina). Cross-platform comparison revealed 29 concordantly detected CNAs, including known recurrent alterations, which confirmed that all platforms are powerful tools when screening for large aberrations. However, detection of 32 additional regions present in 2-3 platforms illustrated a discrepancy in detection of small CNAs, which often involved reported copy-number variations. LOH analysis using dChip revealed concordance of mainly large regions, but showed numerous, small nonoverlapping regions and LOH escaping detection. Evaluation of baseline variation and copy-number ratio response showed the best performance for the Agilent platform and confirmed the robustness of BAC arrays. Accordingly, these platforms demonstrated a higher degree of platform-specific CNAs. The SNP arrays displayed higher technical variation, although this was compensated by high density of elements. Affymetrix detected a higher degree of CNAs compared to Illumina, while the latter showed a lower noise level and higher detection rate in the LOH analysis. Large-scale studies of genomic aberrations are now feasible, but new tools for LOH analysis are requested.
|
|
| 52. |
- Gunnarsson-Östling, Ulrika, 1977-, et al.
(författare)
-
Dags för miljörättvisepolitik
- 2013
-
Ingår i: Tiden. - 0040-6759. ; :2-3
-
Tidskriftsartikel (populärvet., debatt m.m.)
|
|
| 53. |
|
|
| 54. |
|
|
| 55. |
- Hagbert, Pernilla, Dr, 1986-, et al.
(författare)
-
Futures Beyond GDP Growth : Final report from the research program 'Beyond GDP Growth: Scenarios for sustainable building and planning'
- 2019
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- A future society no longer based on economic growth – what would that look like?The research program “Beyond GDP Growth: Scenarios for sustainable building and planning” (www.bortombnptillvaxt.se) is a strong research environment funded by the Swedish Research CouncilFormas, which has run between 2014 and 2018. In collaboration with societal partners, the program hasgathered researchers from diferent disciplines to explore key issues and conditions for planning for asustainable future beyond GDP growth. This is a relevant contribution to a largely under-researchedarea, where few scientific studies have explored what a sustainable society could look like, and what asustainable economy that is not based on growth might actually mean.In economic and political discussions, the notion of continuous economic growth is often taken forgranted and seen as a prerequisite for a safe and sustainable societal development. At the same time,a blind faith in and expectations surrounding growth can constitute a threat to the development of asustainable society if growth declines. Also an optimistic prognosis from the OECD indicates that it islikely that future GDP growth will be lower than what has come to be seen as the normal level duringthe second half of the 20th century. Declining economic growth could mean risks for increased socialgaps and unemployment. However, economic models show that the possibilities for handling these risksincrease if there is an awareness of them, and if this is addressed politically. Therefore, it is important tonot just assume continued economic growth, but to plan also for alternative scenarios.A starting point for the research program has been an understanding of the significant transitionsneeded to approach a safe and just operating space for humanity within planetary boundaries. Fourgoals that should be met in order to consider the societal development sustainable were specified: twoenvironmental goals related to climate and land use, and two social goals regarding power, influence andparticipation, and welfare and resource security.Four scenarios for Sweden 2050 were developed, which show the diferent directions society could taketo reach the set sustainability goals. The scenarios illustrate future societies that do not have to build onthe current economic logic, but that instead are centred around four alternative strategies:Collaborative EconomyLocal Self-SufciencyAutomation for Quality of LifeCircular Economy in the Welfare StateSo, can we reach the selected sustainability targets in the four future scenarios? A transformation ofhistorical proportions are needed – and it needs to start immediately. According to the sustainabilityassessment conducted within the project, the environmental goals of climate and land use can be reachedin all scenarios, even though it demands changing multiple parameters at the same time. Nothing pointsto it being impossible or generally difcult to achieve the social goals in the four scenarios, however theremight be diferent aspects that are particularly tricky. There are both development potentials and risks,which can be diametrically opposite for diferent social groups and parts of the country, depending onthe local prerequisites.Many diferent images of sustainable futures are needed. The scenarios should be seen as a tool fordiscussion and analysis when it comes to planning for a sustainable societal development beyondGDP growth. They challenge notions of what is possible, what changes that can and should be made,6what decisions that are needed and what should be prioritized. The scenarios all suggest a largechange compared the current development trajectory, and for example all point towards the need forredistribution of resources. It might involve economic resources, but could also relate to power andinfluence over production, or the possibility to use land for production of food, materials and energy.This redistribution could happen according to diferent principles in the diferent scenarios.In all the scenarios, the consumption of goods and of meat is reduced. Flight travel also needs to bedrastically reduced to reach the climate target. There is furthermore a need for reducing the constructionof both housing and road infrastructure, although to varying extents in the four scenarios. Other aspectssuch as working hours, the organization of welfare systems, the characteristics of the built environmentand the amount of infrastructure needed are on the other hand diferent in the diferent scenarios.The research program has explored what a development that isn't based on economic growth, in linewith the strategies that are depicted in the scenarios, would mean for rural as well as urban conditions.Three case study municipalities were selected with regards to their diferent geographical location,built form, economic development and size of the population: Övertorneå, Alingsås and Malmö. Insome sub-studies in these diferent contexts, descriptions emerged of cognitive as well as structuralbarriers, a sense of powerlessness and a weak capacity for transition among diferent actors. This isconnected to expectations and general assumptions regarding growth, partly irrespective of the context.Municipalities and companies to a large extent plan for and expect a societal development that buildsupon a further expansion of infrastructure, transport and consumption. Despite visions for sustainabledevelopment, in practice this often leads to a reproduction of current unsustainable structures and waysof life.At the same time, specific empirical studies within the project point toward stories of self-sufciency,of regional upswings and that the population is more important than GDP. There is an increasedawareness and a multitude of examples of experimenting with new sustainable practices that constituteseeds for change. Critiques against planning for continuous growth is being taken more seriously andclearer political visions are demanded. New forms of organizing the economy, society and welfare arealso being developed. Some examples include working from a perspective on socio-ecological justice,integration of sustainability targets in all planning, and developing new roles for consumers andproducers. These ideas can be seen as windows of opportunity, but also show that change can happenwithin the current system.The future means change. In this research program, we point towards some possible futures that aimat reaching certain sustainability targets. The scenarios and the discussion and analysis that they havebrought about show that there is an opportunity to move towards a sustainable development withmaintained or even increased well-being – provided that the understanding of well-being is based onother values than those of our current society. For these possible future trajectories to gain support,there is a need of political instruments and measures that actively drive the development towards a justand safe operating space for humanity
|
|
| 56. |
- Hansson, Karin, et al.
(författare)
-
Simultaneous determination of folate and methotrexate metabolites in serum by LC-MS/MS during high-dose methotrexate therapy
- 2021
-
Ingår i: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier BV. - 1570-0232. ; 1186
-
Tidskriftsartikel (refereegranskat)abstract
- High-dose methotrexate (HDMTX) is a central component in the treatment of acute lymphoblastic leukemia, osteosarcoma, and some lymphomas and brain tumors. MTX is given at lethal doses and then is followed by rescue treatment with folinic acid (FA). Despite FA rescue, many patients suffer severe toxicity. The pharmacokinetics of FA rescue have not been sufficiently studied. However, optimization of FA rescue could potentially increase anti-tumor effects, whilst decreasing organ toxicity. Here, we describe our efforts to establish and optimize a liquid chromatography tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of five essential components of the folate cycle, as well as MTX and its two metabolites. The method was applied to 6 individual patients receiving HDMTX, with 3 or 4 measurements for each patient. The method allows analysis of samples that were initially frozen. This notion, together with the test results in the 6 pilot patients, shows the feasibility of this method to study MTX and FA pharmacokinetics during HDMTX treatment. The method has the potential to optimize HDMTX and FA rescue treatment in individual patients.
|
|
| 57. |
- Hellström-Westas, Lena, et al.
(författare)
-
Short term effects of incubator covers on quiet sleep in stable premature infants
- 2001
-
Ingår i: Acta Pædiatrica. - : Wiley. - 1651-2227 .- 0803-5253. ; 90:9, s. 1004-1008
-
Tidskriftsartikel (refereegranskat)abstract
- Incubator covers are increasingly being used in neonatal care as part of minimal disturbance strategies. The aim of this study was to examine possible effects of incubator covers on sleep patterns in stable premature infants. Quiet sleep (QS) can be investigated by amplitude-integrated electroencephalography (aEEG) at 32–34 weeks gestational age. In nine premature infants (gestational ages 26–32 weeks, median 29) QS periods were measured at a postconceptional age of 32–34 weeks (median 34) during two consecutive 24 h periods, one period with a padded dark cover over the incubator and one period without the cover, in a randomized order. There were no significant differences between the two 24 h periods (with incubator cover and without cover, respectively) regarding the duration of the QS periods, the percentage of QS of the total recording time (% QS) or the duration of QS intervals. However, there was a positive correlation between postnatal age in days and the mean duration of QS periods when incubator covers were used (r = 0.90, p = 0.001). When the covers were used there was a difference between the girls and the boys in the duration of QS intervals (p = 0.032); the QS intervals increased in the five girls from median (range) 63.2 (49.4–94.6) min to 77.2 (59.3–100.9) min (p = 0.043). There was no difference in the duration of QS periods between girls and boys. Conclusion: Incubator covers seem to have some short-term effects on sleep quality in premature infants but the clinical significance and possible long-term effects are not known.
|
|
| 58. |
- Hellström Ängerud, Karin, et al.
(författare)
-
Differences in symptoms, first medical contact and pre-hospital delay times between patients with ST- and non-ST-elevation myocardial infarction
- 2019
-
Ingår i: European Heart Journal. - : Sage Publications. - 2048-8726 .- 2048-8734. ; 8:3, s. 201-207
-
Tidskriftsartikel (refereegranskat)abstract
- Aim: In ST-elevation myocardial infarction, time to reperfusion is crucial for the prognosis. Symptom presentation in myocardial infarction influences pre-hospital delay times but studies about differences in symptoms between patients with ST-elevation myocardial infarction and non-ST-elevation myocardial infarction are sparse and inconclusive. The aim was to compare symptoms, first medical contact and pre-hospital delay times in patients with ST-elevation myocardial infarction and non-ST-elevation myocardial infarction.Methods and results: This multicentre, observational study included 694 myocardial infarction patients from five hospitals. The patients filled in a questionnaire about their pre-hospital experiences within 24 h of hospital admittance. Chest pain was the most common symptom in ST-elevation myocardial infarction and non-ST-elevation myocardial infarction (88.7 vs 87.0%, p=0.56). Patients with cold sweat (odds ratio 3.61, 95% confidence interval 2.29–5.70), jaw pain (odds ratio 2.41, 95% confidence interval 1.04–5.58), and nausea (odds ratio 1.70, 95% confidence interval 1.01–2.87) were more likely to present with ST-elevation myocardial infarction, whereas the opposite was true for symptoms that come and go (odds ratio 0.58, 95% confidence interval 0.38-0.90) or anxiety (odds ratio 0.52, 95% confidence interval 0.29–0.92). Use of emergency medical services was higher among patients admitted with ST-elevation myocardial infarction. The pre-hospital delay time from symptom onset to first medical contact was significantly longer in non-ST-elevation myocardial infarction (2:05 h vs 1:10 h, p=0.001).Conclusion: Patients with ST-elevation myocardial infarction differed from those with non-ST-elevation myocardial infarction regarding symptom presentation, ambulance utilisation and pre-hospital delay times. This knowledge is important to be aware of for all healthcare personnel and the general public especially in order to recognise symptoms suggestive of ST-elevation myocardial infarction and when to decide if there is a need for an ambulance.
|
|
| 59. |
- Hellström Ängerud, Karin, et al.
(författare)
-
Differences in symptoms in relation to myocardial infarction.
- 2016
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Background: In myocardial infarction (MI) rapid diagnosis and treatment is crucial for the prognosis. Previous research has found that symptom presentation influence pre hospital delay times but studies about differences in MI symptoms between patients with ST-elevation myocardial infarction (STEMI) and non ST-elevation myocardial infarction (NSTEMI) are sparse and inconclusive. To enhance the understanding of symptom presentation in regard to MI type, we aimed to describe symptoms in relation to MI type and to find predictors of STEMI versus NSTEMI in patients with MI.Methods: Patients with MI (n=694) from the SymTime study were included. SymTime was a multicentre cross-sectional study of symptoms and actions in the prehospital phase of MI and data were collected using a previously validated questionnaire administered to MI patients within 24 h of admission to hospital.Results: Patients with STEMI were younger, more often men and smokers. Patients with NSTEMI were more likely to have a history of hypertension, MI and stroke. Chest pain was the most common symptom in both groups. Pain, discomfort, or pressure located in the jaw or teeth, vertigo/pre-syncope, cold sweat and nausea/vomiting were significantly more frequent in patients with STEMI (Table 1). In a multivariate logistic regression model patients with STEMI were more likely to present with cold sweat (OR 4.13, 95% CI 2.71–6.29) jaw pain (OR 2.14, 95% CI 1.02–4.50), and nausea (OR 2.01, 95% CI 1.20–3.33), and less likely to have a history of stroke (OR 0.35, 95% CI 0.15–0.84), fluctuating symptoms (OR 0.54, 95% CI 0.36–0.83) and anxiety (OR 0.54, 95% CI 0.32–0.92) compared to patients with NSTEMI.Conclusion: Patients with STEMI differed significantly from those with NSTEMI regarding symptom presentation. This knowledge is important for health care personnel to recognize symptoms alarming for STEMI when evaluating patients with MI symptoms.
|
|
| 60. |
|
|
