| 11. |
- Chondrelli, Niki, et al.
(författare)
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Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo)
- 2024
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Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 14
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Tidskriftsartikel (refereegranskat)abstract
- Batrachochytrium dendrobatidis (Bd) is a fungal pathogen that has decimated amphibian populations worldwide for several decades. We examined the changes in gene expression in response to Bd infection in two populations of the common toad, Bufo bufo, in a laboratory experiment. We collected B. bufo eggs in southern and northern Sweden, and infected the laboratory-raised metamorphs with two strains of the global panzoonotic lineage Bd-GPL. Differential expression analysis showed significant differences between infected and control individuals in both liver and skin. The skin samples showed no discernible differences in gene expression between the two strains used, while liver samples were differentiated by strain, with one of the strains eliciting no immune response from infected toads. Immune system genes were overexpressed in skin samples from surviving infected individuals, while in liver samples the pattern was more diffuse. Splitting samples by population revealed a stronger immune response in northern individuals. Differences in transcriptional regulation between populations are particularly relevant to study in Swedish amphibians, which may have experienced varying exposure to Bd. Earlier exposure to this pathogen and subsequent adaptation or selection pressure may contribute to the survival of some populations over others, while standing genetic diversity in different populations may also affect the infection outcome.
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| 12. |
- Cortazar-Chinarro, Maria, et al.
(författare)
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Antimicrobial peptide and sequence variation along a latitudinal gradient in two anurans
- 2020
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Ingår i: BMC Genetics. - : BMC. - 1471-2156. ; 21:1
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Tidskriftsartikel (refereegranskat)abstract
- Background While there is evidence of both purifying and balancing selection in immune defense genes, large-scale genetic diversity in antimicrobial peptides (AMPs), an important part of the innate immune system released from dermal glands in the skin, has remained uninvestigated. Here we describe genetic diversity at three AMP loci (Temporin, Brevinin and Palustrin) in two ranid frogs (Rana arvalis and R. temporaria) along a 2000 km latitudinal gradient. We amplified and sequenced part of the Acidic Propiece domain and the hypervariable Mature Peptide domain ( 150-200 bp) in the three genes using Illumina Miseq and expected to find decreased AMP genetic variation towards the northern distribution limit of the species similarly to studies on MHC genetic patterns. Results We found multiple loci for each AMP and relatively high gene diversity, but no clear pattern of geographic genetic structure along the latitudinal gradient. We found evidence of trans-specific polymorphism in the two species, indicating a common evolutionary origin of the alleles. Temporin and Brevinin did not form monophyletic clades suggesting that they belong to the same gene family. By implementing codon evolution models we found evidence of strong positive selection acting on the Mature Peptide. We also found evidence of diversifying selection as indicated by divergent allele frequencies among populations and high Theta k values. Conclusion Our results suggest that AMPs are an important source of adaptive diversity, minimizing the chance of microorganisms developing resistance to individual peptides.
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| 13. |
- Cortazar-Chinarro, Maria, et al.
(författare)
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Association between the skin microbiome and MHC class II diversity in an amphibian
- 2024
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Ingår i: Molecular Ecology. - : John Wiley & Sons. - 0962-1083 .- 1365-294X. ; 33:1
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Tidskriftsartikel (refereegranskat)abstract
- Microbiomes play an important role in determining the ecology and behaviour of their hosts. However, questions remain pertaining to how host genetics shape microbiomes, and how microbiome composition influences host fitness. We explored the effects of geography, evolutionary history and host genetics on the skin microbiome diversity and structure in a widespread amphibian. More specifically, we examined the association between bacterial diversity and composition and the major histocompatibility complex class II exon 2 diversity in 12 moor frog (Rana arvalis) populations belonging to two geographical clusters that show signatures of past and ongoing differential selection. We found that while bacterial alpha diversity did not differ between the two clusters, MHC alleles/supertypes and genetic diversity varied considerably depending on geography and evolutionary history. Bacterial alpha diversity was positively correlated with expected MHC heterozygosity and negatively with MHC nucleotide diversity. Furthermore, bacterial community composition showed significant variation between the two geographical clusters and between specific MHC alleles/supertypes. Our findings emphasize the importance of historical demographic events on hologenomic variation and provide new insights into how immunogenetic host variability and microbial diversity may jointly influence host fitness with consequences for disease susceptibility and population persistence.
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| 14. |
- Cortazar-Chinarro, Maria, et al.
(författare)
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Drift, selection, or migration? : Processes affecting genetic differentiation and variation along a latitudinal gradient in an amphibian
- 2017
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Ingår i: BMC Evolutionary Biology. - : Springer Science and Business Media LLC. - 1471-2148. ; 17
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Tidskriftsartikel (refereegranskat)abstract
- Background: Past events like fluctuations in population size and post-glacial colonization processes may influence the relative importance of genetic drift, migration and selection when determining the present day patterns of genetic variation. We disentangle how drift, selection and migration shape neutral and adaptive genetic variation in 12 moor frog populations along a 1700 km latitudinal gradient. We studied genetic differentiation and variation at a MHC exon II locus and a set of 18 microsatellites. Results: Using outlier analyses, we identified the MHC II exon 2 (corresponding to the beta-2 domain) locus and one microsatellite locus (RCO8640) to be subject to diversifying selection, while five microsatellite loci showed signals of stabilizing selection among populations. STRUCTURE and DAPC analyses on the neutral microsatellites assigned populations to a northern and a southern cluster, reflecting two different post-glacial colonization routes found in previous studies. Genetic variation overall was lower in the northern cluster. The signature of selection on MHC exon II was weaker in the northern cluster, possibly as a consequence of smaller and more fragmented populations. Conclusion: Our results show that historical demographic processes combined with selection and drift have led to a complex pattern of differentiation along the gradient where some loci are more divergent among populations than predicted from drift expectations due to diversifying selection, while other loci are more uniform among populations due to stabilizing selection. Importantly, both overall and MHC genetic variation are lower at northern latitudes. Due to lower evolutionary potential, the low genetic variation in northern populations may increase the risk of extinction when confronted with emerging pathogens and climate change.
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| 15. |
- Cortazar-Chinarro, Maria
(författare)
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Immunogenetic variation along the latitudinal gradient in Scandinavian anuran species : Evolutionary processes, demography and infection
- 2019
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The evolutionary and demographic processes affecting how genetic variation is partitioned and distributed over large geographical scales is of fundamental importance for our understanding of how organisms may adapt to their environments. Northern peripheral populations generally have lower genetic variation and individuals in these populations may therefore face difficulties adapting to their local environment. At northern latitudes lack of genetic variation could be detrimental in face of newly emerging diseases as a result of anthropogenic actions and warmer climate in these areas. In this thesis, I explore genetic variation and the contemporary evolutionary processes affecting genes involved in the adaptive immune defense (Major Histocompatibility Complex; MHC) and the innate immune defense (AMP; Antimicrobial Peptides) over a large geographical gradient in anuran species (paper I, II and IV). I study signatures of historical selection on the MHC class II exon 2 and AMP (Temporin, Brevinin and Palustrin) sequences in the Signal Peptide and the Acidic Propiece domains (paper II and III). Finally, I investigate potential associations between specific MHC class II exon 2 alleles and a chytrid fungus infection (Bd) in common toads (Bufo bufo) (paper IV). The results reveal that genetic variation of MHC class II exon 2 decreases towards northern latitudes in R. arvalis and B. bufo and have been shaped by complex evolutionary processes (drift, selection, migration) affected by different demographic scenarios. On the other hand, AMP nucleotide variation is divergent among geographical areas, but there is no clear geographical pattern along the same gradient, suggesting diversifying selection as the main force shaping genetic variation. Finally, I found an effect of two specific MHC class II exon 2 alleles on survival in juvenile B. bufo when infected with Bd. In summary, my thesis unravels the complex patterns shaping genetic diversity at large scales. My results may guide conservation practices aiming to prevent amphibian mass mortality events on-going all over the world.
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| 16. |
- Cortazar-Chinarro, Maria, 1985-, et al.
(författare)
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Latitudinal MHC variation and haplotype associated differential survival in response to experimental infection of two strains of Bd-GPL in common toads
- 2024
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- While both innate and adaptive immune system mechanisms have been implicated in resistance against the chytrid fungusBatrachochytrium dendrobatitis, studies on the role of specific MHC haplotypes onBdinfection are rare. Here, we studied latitudinal variation in MHC Class IIB loci along a latitudinal gradient from southern to northern Sweden in common toads,Bufo bufo. Swedish toad populations had fewer MHC Class IIB haplotypes compared to a previous study of populations in Britain. Furthermore, we found MHC diversity to decline from south to the north within Sweden. The low diversity may compromise the ability of northern populations to fight emerging disease, such as the chytrid fungusBd. In a laboratory experiment, we infected newly metamorphosed toads with two strains of the Global Pandemic Lineage of the fungus (Bd-GPL) and compared survival with sham controls. We foundBd-infected toads had lower survival compared to controls. Survival was dependent onBd-strain and whether experimental toads where collected in the south or the north of Sweden with lower survival in northern individuals. MHC diversity was lower in toads of northern origin, all northern animals being monomorphic for a single MHC haplotype, whereas we found seven different haplotypes in southern animals. Survival of infected animals was dependent on bothBd-strain and MHC haplotype suggesting differential infection dynamics depending on bothBd-strain and host MHC characteristics.
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| 17. |
- Cortazar-Chinarro, Maria, et al.
(författare)
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Major Histocompatibility Complex Variation and Haplotype Associated Survival in Response to Experimental Infection of Two Bd-GPL Strains Along a Latitudinal Gradient
- 2022
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Ingår i: Frontiers in Ecology and Evolution. - : Frontiers Media S.A.. - 2296-701X. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- While both innate and adaptive immune system mechanisms have been implicated in resistance against the chytrid fungus Batrachochytrium dendrobatidis (Bd), studies on the role of specific MHC haplotypes on Bd infection are rare. Here, we studied variation in MHC Class IIB loci in the common toad Bufo bufo along a latitudinal gradient across Sweden. In general, Swedish toad populations had few MHC Class IIB haplotypes and MHC diversity declined from south (13 haplotypes) to the north (four haplotypes). The low diversity may compromise the ability of northern populations to fight emerging disease, such as Bd. In a laboratory experiment, we infected newly metamorphosed toads with two strains of the Global Pandemic Lineage of the fungus (Bd-GPL) and compared survival with sham controls. Bd-infected toads had lower survival compared to controls. Moreover, survival was dependent on the Bd-strain and northern toads had lower Bd-mediated survival than southern individuals. MHC diversity was lower in northern toads. All northern experimental animals were monomorphic for a single MHC haplotype, whereas we found seven different haplotypes in southern experimental animals. In southern toads, survival was dependent on both Bd-strain and MHC haplotype suggesting differential infection dynamics depending on both Bd-strain and host immune system characteristics.
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| 18. |
- Cortazar-Chinarro, Maria, et al.
(författare)
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Signatures of historical selection on MHC reveal different selection patterns in the moor frog (Rana arvalis)
- 2018
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Ingår i: Immunogenetics. - : Springer Science and Business Media LLC. - 0093-7711 .- 1432-1211. ; 70:7, s. 477-484
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Tidskriftsartikel (refereegranskat)abstract
- MHC genes are key components in disease resistance and an excellent system for studying selection acting on genetic variation in natural populations. Current patterns of variation in MHC genes are likely to be influenced by past and ongoing selection as well as demographic fluctuations in population size such as those imposed by post-glacial recolonization processes. Here, we investigated signatures of historical selection and demography on an MHC class II gene in 12 moor frog populations along a 1700-km latitudinal gradient. Sequences were obtained from 207 individuals and consecutively assigned into two different clusters (northern and southern clusters, respectively) in concordance with a previously described dual post-glacial colonization route. Selection analyses comparing the relative rates of non-synonymous to synonymous substitutions (dN/dS) suggested evidence of different selection patterns in the northern and the southern clusters, with divergent selection prevailing in the south but uniform positive selection predominating in the north. Also, models of codon evolution revealed considerable differences in the strength of selection: The southern cluster appeared to be under strong selection while the northern cluster showed moderate signs of selection. Our results indicate that the MHC alleles in the north diverged from southern MHC alleles as a result of differential selection patterns.
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| 19. |
- Dahl, Emma, et al.
(författare)
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Geographic variation in corticosterone response to chronic predator stress in tadpoles
- 2012
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Ingår i: Journal of Evolutionary Biology. - : Wiley. - 1010-061X .- 1420-9101. ; 25:6, s. 1066-1076
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Tidskriftsartikel (refereegranskat)abstract
- Chronic stress often affects growth and development negatively, and these effects are often mediated via glucocorticoid hormones, which elevate during stress. We investigated latitudinal variation in corticosterone (CORT) response to chronic predator stress in Rana temporaria tadpoles along a 1500-km latitudinal cline in Sweden tadpoles, in a laboratory experiment. We hypothesized that more time-constrained high-latitude populations have evolved a lower CORT response to chronic stress to maintain higher growth under stressful conditions. Southern tadpoles had higher CORT content in response to predators after 1 day of exposure, whereas there was no increase in CORT in the northern populations. Two weeks later, there were no predator-induced CORT elevations. Artificially elevated CORT levels strongly decreased growth, development and survival in both northern and southern tadpoles. We suggest that the lower CORT response in high-latitude populations can be connected with avoidance of CORT-mediated reduction in growth and development, but also discuss other possible explanations.
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| 20. |
- Dahl, Emma, et al.
(författare)
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Is growth hormone expression correlated with variation in growth rate along a latitudinal gradient in Rana temporaria?
- 2011
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Ingår i: Journal of Zoology. - : Wiley. - 0952-8369 .- 1469-7998. ; 285:2, s. 85-92
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Tidskriftsartikel (refereegranskat)abstract
- In ectotherms, decreasing season length and lower temperature towards higher latitudes often favour higher growth and developmental rates. However, the underlying physiological mechanisms and particularly the hormonal correlates of clinal variation remain unexplored. The growth hormone (GH) plays a crucial role in growth of all vertebrates and high expression of GH is associated with rapid growth in many species. We tested the hypothesis that GH expression is correlated with a latitudinal gradient in growth in Scandinavian Rana temporaria tadpoles. Using quantitative polymerase chain reaction, we measured GH and growth hormone receptor (GHR) expression at two time points from laboratory-raised tadpoles originating from eight populations collected along the latitudinal gradient. To explore latitudinal differences in stress-induced changes in GH expression, we also compared GH expression in tadpoles raised with and without predators. In accordance with previous studies we found a clear latitudinal gradient in growth. There were no latitudinal effects, or predator-induced effects on GH or GHR expression. However, there was some indication for among-population variation in GH expression. The lack of a latitudinal pattern in GH and GHR expression may be due to that the growth promoting effects of GH is dependent on other factors including insulin-like growth factor-I (IGF), IGF-binding proteins or prolactin. Further studies on these factors may provide insight on the proximal mechanisms of differences in growth in R. temporaria tadpoles.
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