| 61. |
- Johansson, J, et al.
(författare)
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A synthetic surfactant based on a poly-Leu SP-C analog and phospholipids: effects on tidal volumes and lung gas volumes in ventilated immature newborn rabbits
- 2003
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Ingår i: Journal of applied physiology (Bethesda, Md. : 1985). - : American Physiological Society. - 8750-7587 .- 1522-1601. ; 95:5, s. 2055-2063
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Tidskriftsartikel (refereegranskat)abstract
- Available surfactants for treatment of respiratory distress syndrome in newborn infants are derived from animal lungs, which limits supply and poses a danger of propagating infectious material. Poly-Val→poly-Leu analogs of surfactant protein (SP)-C can be synthesized in large quantities and exhibit surface activity similar to SP-C. Here, activity of synthetic surfactants containing a poly-Leu SP-C analog (SP-C33) was evaluated in ventilated premature newborn rabbits. Treatment with 2.5 ml/kg body wt of 2% (wt/wt) SP-C33 in 1,2-dipalmitoyl- sn-3-glycero phosphoryl choline (DPPC)-1-palmitoyl-2-oleoyl- sn-3-glycero phosphoryl choline (POPC)-1-palmitoyl-2-oleoyl- sn-3-glycero phosphoryl glycerol (POPG), 68:0:31, 68:11:20, or 68:16:15 (wt/wt/wt) suspended at 80 mg/ml gave tidal volumes (Vt) of 20-25 ml/kg body wt, with an insufflation pressure of 25 cmH2O and no positive end-expiratory pressure (PEEP), comparable to the Vt for animals treated with the porcine surfactant Curosurf. Nontreated littermates had a Vt of ∼2 ml/kg body wt. The Vt for SP-C33 in DPPC-egg phosphatidylglycerol-palmitic acid [68:22:9 (wt/wt/wt)], DPPC-POPG-palmitic acid [68:22:9 (wt/wt/wt)], and DPPC-POPC-POPG [6:2:2 (wt/wt/wt)] was 15-20 ml/kg body wt. Histological examination of lungs from animals treated with SP-C33-based surfactants showed incomplete, usually patchy air expansion of alveolar spaces associated with only mild airway epithelial damage. Lung gas volume after 30 min of mechanical ventilation were more than threefold larger in animals treated with Curosurf than in those receiving SP-C33 in DPPC-POPC-POPG, 68:11:20. This difference could be largely counterbalanced by ventilation with PEEP (3-4 cmH2O). An artificial surfactant based on SP-C33 improves Vt in immature newborn animals ventilated with standardized peak pressure but requires PEEP to build up adequate lung gas volumes.
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| 62. |
- Johnson, Taryn, et al.
(författare)
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Working Ethically with Ancient DNA from Composites in the United States
- 2024
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Ingår i: Advances in archaeological practice. - 2326-3768.
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Tidskriftsartikel (refereegranskat)abstract
- This article discusses ethical frameworks for planning and implementing composite research in the United States. Composites, defined here as archaeological materials with multiple genetic sources, include materials such as sediment, coprolites, birch pitch, and dental calculus. Although composites are increasingly used in genetic research, the ethical considerations of their use in ancient DNA studies have not been widely discussed. Here, we consider how composites' compositions, contexts, and potential to act as proxies can affect research plans and offer an overview of the primary ethical concerns of ancient DNA research. It is our view that ethical principles established for analyses of Ancestral remains and related materials can be used to inform research plans when working with composite evidence. This work also provides a guide to archaeologists unfamiliar with genetics analyses in planning research when using composite evidence from the United States with a focus on collaboration, having a clear research plan, and using lab methods that provide the desired data with minimal destruction. Following the principles discussed in this article and others allows for engaging in composite research while creating and maintaining positive relationships with stakeholders. El presente trabajo analiza las preocupaciones eticas para la planificacion e implementacion de investigaciones compuestas en los Estados Unidos. Los compuestos, definidos aqui como muestras arqueologicas con multiples fuentes geneticas, incluyen materiales como sedimentos, paleofecas, brea de abedul y calculo dental. Sin embargo, si bien los compuestos se han utilizado cada vez mas en la investigacion genetica arqueologica, las consideraciones eticas de su uso en estudios de aADN no se han discutido ampliamente. Aqui consideramos como las composiciones, los contextos y el potencial de los compuestos para actuar como sustitutos pueden afectar los planes de investigacion y ofrecer una vision general de las principales preocupaciones eticas de la investigacion del ADN antiguo. Es la opinion de los autores que los principios eticos establecidos para los analisis de restos humanos y materiales relacionados se pueden utilizar para informar los planes de investigacion cuando se trabaja con evidencia compuesta. Este trabajo ofrece tambien una guia para planificar la investigacion cuando se utiliza evidencia compuesta con un enfoque en la colaboracion, en planes de investigacion claros y uso de metodos de laboratorio que proporcionen los datos deseados con una destruccion minima de la muestra. Seguir los principios descritos en este documento permite participar en la investigacion compuesta sin dejar de lado la creacion y mantencion de relaciones positivas con las partes interesadas.
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| 63. |
- Kaufman, DS, et al.
(författare)
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Technical note: Open-paleo-data implementation pilot-The PAGES 2k special issue
- 2018
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Ingår i: Climate of the Past. - 1814-9324 .- 1814-9332. ; 14, s. 593-600
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Tidskriftsartikel (refereegranskat)abstract
- Data stewardship is an essential element of the publication process. Knowing how to enact data polices that are described only in general terms can be difficult, however. Examples are needed to model the implementation of open-data polices in actual studies. Here we explain the procedure used to attain a high and consistent level of data stewardship across a special issue of the journal Climate of the Past. We discuss the challenges related to (1) determining which data are essential for public archival, (2) using data generated by others, and (3) understanding data citations. We anticipate that open-data sharing in paleo sciences will accelerate as the advantages become more evident and as practices that reduce data loss become the accepted convention.
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