| 71. |
- Lindqvist, Anna-Karin, et al.
(författare)
-
The Praise and Price of Pokémon GO : A Qualitative Study of Children's and Parents' Experiences.
- 2018
-
Ingår i: JMIR Serious Games. - : JMIR Publications. - 2291-9279. ; 6:1
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Physical activity has multiple health benefits; however, the majority of children around the world do not attain the recommended levels of daily physical activity. Research has shown that the game Pokémon GO has increased the amount of physical activity of players and that the game has the potential to reach populations that traditionally have low levels of physical activity. Therefore, there is a need to understand which game components can promote initial and sustained physical activity. By using a qualitative research approach, it is possible to achieve rich descriptions and enhance a deep understanding of the components promoting physical activity among children in a game such as Pokémon GO.Objective: The objective of this study was to explore children’s and parents’ experiences playing Pokémon GO.Methods: Eight families comprising 13 children (aged 7-12 years) and 9 parents were selected using purposeful sampling. Data collected using focus groups were analyzed using qualitative latent content analysis.Results: The following three themes were revealed: (1) exciting and enjoyable exploration; (2) dangers and disadvantages; and (3) cooperation conquers competition. The first centers around the present and possible future aspects of Pokémon GO that promote physical activity. The second focuses on unwanted aspects and specific threats to safety when playing the game. The third shows that cooperation and togetherness are highly valued by the participants and that competition is fun but less important.Conclusions: Components from Pokémon GO could enhance the efficacy of physical activity interventions. Cooperation and exploration are aspects of the game that preferably could be transferred into interventions aimed at promoting children’s physical activity.
|
|
| 72. |
- Lindqvist, Anna-Karin, et al.
(författare)
-
The road to success - aktiva skoltransporter
- 2019
-
Konferensbidrag (populärvet., debatt m.m.)abstract
- Endast omkring 30% av svenska barn når rekommendationen för fysisk aktivitet och bara hälften av barnen använder aktiva skoltransporter (AST). Förutom den samhälleliga utmaningen som detta innebär för barns hälsa och kognitiv förmåga, har det också en negativ inverkan på klimatet och trafiksäkerhet nära skolorna. Dessutom följer barns fysiska vanor med in i vuxenlivet och därmed har den fysiska inaktiviteten på sikt en betydande hälsoekonomisk negativ effekt. Vi arbetar med ett skolbaserat koncept som bygger på empowerment och gamification. Det slutgiltiga målet är en innovativ digital lösning för en hållbar beteendeförändring avseende AST och att implementeringen av innovationen ökar användandet av AST bland svenska barn från dagens 52% till 80%.
|
|
| 73. |
- Lindqvist Appell, Malin, 1976-, et al.
(författare)
-
Explaining TPMT genotype/phenotype discrepancy by haplotyping of TPMT*3A and identification of a novel sequence variant, TPMT*23
- 2007
-
Ingår i: Pharmacogenetics and Genomics. - 1744-6872. ; 17:10, s. 891-895
-
Tidskriftsartikel (refereegranskat)abstract
- Thiopurine methyltransferase (TPMT) is a polymorphic enzyme involved in the metabolism of thiopurine drugs. Owing to polymorphisms in the TPMT gene (TPMT*2-*22), the enzyme activity varies interindividually. Patients with reduced TPMT activity may develop adverse reactions when treated with standard doses of thiopurines. This work focuses on a TPMT genotype/phenotype discrepancy found in a patient during routine testing. The patient displayed very low TPMT enzyme activity and she was genotyped by pyrosequencing as being heterozygous for the 460G>A and 719A>G polymorphisms (TPMT*3A). Complete sequencing in combination with haplotyping of the TPMT gene revealed a novel sequence variant, 500C>G, on one allele and TPMT*3A on the other allele, giving rise to the novel genotype TPMT*3A/*23. When investigating the patient's relatives, they too had the TPMT*3A/*23 genotype in combination with low enzyme activity. We conclude that this novel variant allele affects enzyme activity, as the individuals carrying it had almost undetectable TPMT activity. © 2007 Lippincott Williams & Wilkins, Inc.
|
|
| 74. |
- Mikaelsson, Katarina, et al.
(författare)
-
Physically inactive adolescents’ experiences of engaging in physical activity
- 2020
-
Ingår i: European Journal of Physiotherapy. - : Taylor & Francis. - 2167-9169 .- 2167-9177. ; 22:4, s. 191-196
-
Tidskriftsartikel (refereegranskat)abstract
- Purpose: This study aimed to describe physically inactive adolescents’ experiences and reflections about engaging in physical activity. Methods: Nine graduate students from the third year of upper secondary school (six women and three men) participated in this study. Narrative interviews were used for data collection and qualitative content analysis was used to analyse the interviews. Results: The analysis revealed three themes ‘Acknowledging resistance and barriers to performing physical activity’, ‘Knowing that it is good is not enough’, and ‘Feeling included and accepted is fun and motivating’. These themes show that the adolescent’s experienced barriers, acknowledged pros and cons and identified possibilities to be physically active. Conclusions: Identifying experiences that impact on inactive adolescents’ attitude and willingness to perform physical activity can be useful to understand the needs of the individual. By relating these experiences to the different stages of the transtheoretical model, this study could provide valuable knowledge for designing future interventions to enhance physical activity in this target group.
|
|
| 75. |
- Nandakumar, Kutty Selva, 1965-, et al.
(författare)
-
A dominant suppressive MHC class II haplotype interacting with autosomal genes controls autoantibody production and chronicity of arthritis
- 2011
-
Ingår i: Annals of the Rheumatic Diseases. - London : BMJ Publishing Group Ltd. - 0003-4967 .- 1468-2060. ; 70:9, s. 1664-1670
-
Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To investigate the genetic control of chronic arthritis and collagen epitope specific antibody responses in an experimental model for rheumatoid arthritis. METHODS: The chronic collagen induced arthritis (CCIA) model was used, induced with collagen type II (CII) in mineral oil lacking mycobacterium in BALB/c (n=24), B10.Q (n=44), (BALB/c x B10.Q) F1 (n=85) and B10.Q x (BALB/c x B10.Q) N2 (n=684) mice. Genome-wide genotyping for 190 N2 mice was performed with extreme phenotypes: chronic arthritis that persisted for 4 months or non-affected. Statistical and linkage analysis were performed with R/qtl software using arthritis and serum subphenotypes. RESULTS: (BALB/c x B10.Q) F1 mice were highly prone to develop a chronic relapsing arthritis (66%), whereas both parental strains were relatively resistant: BALB/c (H-2(d); 0%) and B10.Q (H-2(q); 4.5%). CCIA experiments were performed on 684 mice backcrossed to B10.Q; 38% of the mice developed arthritis and more than half of them developed chronic arthritis phenotype. Genome-wide genotyping revealed mainly the major histocompatibility complex (MHC) locus that had an independent and dominant influence on the chronicity. Interestingly, the H2(d) allele had a dominant suppressive effect. This effect overrode the role of other loci as interaction analysis, after conditioning MHC, revealed additional loci, controlling arthritis and autoantibody phenotypes. CONCLUSIONS: A dominant negative influence of specific MHC haplotype (H2(d)) on CCIA was identified. Further, loci controlling the autoantibody response to different CII epitopes were also identified, and it has been shown that these are dependent on MHC and non-MHC genes.
|
|
| 76. |
- Nyström, Michelle, et al.
(författare)
-
Making the right decision for our children's future: Parents' perceptions of active school travel in disadvantaged neighborhoods
- 2023
-
Ingår i: Journal of Transport and Health. - : Elsevier. - 2214-1405 .- 2214-1405. ; 30
-
Tidskriftsartikel (refereegranskat)abstract
- BackgroundChildren's possibility to be physically active is linked to their parent's socioeconomic status. The use of active travel has the potential to increase daily physical activity among children. Parents are the gate-keepers to children using active school travel (AST) and their perceptions has shown to impact children's travel mode. Few studies have explored parents' perceptions about AST in disadvantaged neighborhoods and there is a lack of knowledge of their perceptions of the physical and social environment associated with school travel.PurposeTo explore parents’ perceptions towards AST when living in disadvantaged neighborhoods.MethodsTwelve parents participated in semi-structured interviews, and a qualitative content analysis was used to analyze the data.ResultsThe findings show that parents faced dilemmas, striving to facilitate AST. Parents perceptions are presented as A, B, C categories that are likely to be important when promoting AST in disadvantaged neighborhoods, Acknowledging AST advantages, Balancing barriers, and Creating opportunities to use AST.ConclusionsDespite having a positive attitude towards AST, insecure neighborhoods and social exclusion affect parents'descisions about AST. When promoting AST in disadvantaged neighborhoods, measures to enable AST should include efforts supporting community building, social participation, road safety and ways of promoting bicycling. Engaging children in AST could have a positive influence on their independent mobility, thereby impacting their development and preparing them for the future.
|
|
| 77. |
- Olsson, Lina, et al.
(författare)
-
A case-control study of rheumatoid arthritis identifies an associated single nucleotide polymorphism in the NCF4 gene, supporting a role for the NADPH-oxidase complex in autoimmunity
- 2007
-
Ingår i: Arthritis Research and Therapy. - : Springer Science and Business Media LLC. - 1478-6362 .- 1478-6354. ; 9:5
-
Tidskriftsartikel (refereegranskat)abstract
- ABSTRACT: Rheumatoid arthritis (RA) is a chronic inflammatory disease with a heritability of 60%. Genetic contributions to RA are made by multiple genes, but only a few gene associations have yet been confirmed. By studying animal models, reduced capacity of the NADPH-oxidase (NOX) complex, caused by a single nucleotide polymorphism (SNP) in one of its components (the NCF1 gene), has been found to increase severity of arthritis. To our knowledge, however, no studies investigating the potential role played by reduced reactive oxygen species production in human RA have yet been reported. In order to examine the role played by the NOX complex in RA, we investigated the association of 51 SNPs in five genes of the NOX complex (CYBB, CYBA, NCF4, NCF2, and RAC2) in a Swedish case-control cohort consisting of 1,842 RA cases and 1,038 control individuals. Several SNPs were found to be mildly associated in men in NCF4 (rs729749, P = 0.001), NCF2 (rs789181, P = 0.02) and RAC2 (rs1476002, P = 0.05). No associations were detected in CYBA or CYBB. By stratifying for autoantibody status, we identified a strong association for rs729749 (in NCF4) in autoantibody negative disease, with the strongest association detected in rheumatoid factor negative men (CT genotype versus CC genotype: odds ratio 0.34, 95% confidence interval 0.2 to 0.6; P = 0.0001). To our knowledge, this is the first genetic association identified between RA and the NOX complex, and it supports previous findings from animal models of the importance of reactive oxygen species production capacity to the development of arthritis.
|
|
| 78. |
- Olsson, Lina, et al.
(författare)
-
Copy number variation of the gene NCF1 is associated with Rheumatoid Arthritis.
- 2012
-
Ingår i: Antioxidants & Redox Signaling. - : Mary Ann Liebert Inc. - 1557-7716 .- 1523-0864. ; 16:1, s. 71-78
-
Tidskriftsartikel (refereegranskat)abstract
- Aims The aim of this study was to investigate genetic variants in the gene NCF1 for association with Rheumatoid Arthritis (RA). In rodent models, a single nucleotide polymorphism (SNP) in Ncf1 has been shown to be a major locus regulating severity of arthritis. Ncf1 encodes one of 5 subunits of the NADPH oxidase complex. In humans the genomic structure of NCF1 is complex, excluding it from genome wide association screens and complicating genetic analysis. In addition to copy number variation of NCF1, there are also two non-functional pseudogenes, nearly identical in sequence to NCF1. We have characterised copy number variation and SNPs in NCF1, and investigated these variants for association with RA. Results We find that RA patients are less likely to have an increased copy number of NCF1, 7.6%, compared to 11.6% in controls; P = 0.037. We also show that the T-allele of NCF1-339 (rs13447) is expressed in NCF1 and significantly reduces ROS production. Innovation This is the first finding of genetic association of NCF1 with RA. The detailed characterisation of genetic variants in NCF1 also help elucidate the complexity of the NCF1 gene. Conclusion These data suggest that an increased copy number of NCF1 can be protective against developing RA and add support to previous findings of a role of NCF1 and the NOX2 complex in RA pathogenesis.
|
|
| 79. |
- Oyelere, Solomon Sunday, et al.
(författare)
-
Initial Design and Testing of Multiplayer Cooperative Game to Support Physical Activity in Schools
- 2022
-
Ingår i: Education Sciences. - : MDPI. - 2227-7102. ; 12:2
-
Tidskriftsartikel (refereegranskat)abstract
- ecent studies have shown that children are not adequately physically active and there is a need to increase children’s physical activity. This study describes new opportunities and solutions for using existing games and gamification to increase physical activity among children in Sweden. We adopted the principles of Tic-Tac-Training to redesign, build, and test a classical multiplayer cooperative game, Battleship, to create a PA game that children experience as fun and engaging. The low fidelity prototype of the game was developed using an iterative game development life cycle and tested with 13 young male children aged 8–11 in a real-world informal setting. A mixed-method research approach was used to understand the users’ experiences and the impact of the Battleship-PA game on behavior change regarding physical activity. Research data were collected through audio recordings of interactions, direct observation, and a user experience questionnaire. The results of this study indicate both positive and negative feedback that can be used to improve the game and user experiences. The results from the unfiltered recordings revealed that both teams were competitive, cooperated within their team, and became excited whenever they destroyed opponent’s ships or were close to winning. However, the children felt bored and exhausted when many gamification tasks were repeated several times in a game session. Direct observation indicated that the children enjoyed the physical activities resulting from playing the game. However, participants who had not previously played the classical version of Battleship were confused about the objectives and concept of the game. The analysis of the user experience questionnaire indicated that most children found the game easy to play, motivating, engaging, interactive, fun, cooperative, competitive, and visually appealing. Furthermore, most children agreed that the game helped them to be physically active and strongly agreed that they enjoyed performing the physical activities in the game. Future work is needed to improve the game user interface, gamification elements, and prepare additional physical activity tasks for a rewarding experience.
|
|
| 80. |
- Panayiotou, Christakis, et al.
(författare)
-
Viperin restricts Zika virus and tick-borne encephalitis virus replication by targeting NS3 for proteasomal degradation
- 2018
-
Ingår i: Journal of Virology. - : American Society for Microbiology. - 0022-538X .- 1098-5514. ; 92:7
-
Tidskriftsartikel (refereegranskat)abstract
- Flaviviruses are arthropod-borne viruses that constitute a major global health problem, with millions of human infections annually. Their pathogenesis ranges from mild illness to severe manifestations such as hemorrhagic fever and fatal encephalitis. Type I interferons (IFNs) are induced in response to viral infection and stimulate the expression of interferon-stimulated genes (ISGs), including that encoding viperin (virus-inhibitory protein, endoplasmic reticulum associated, IFN inducible), which shows antiviral activity against a broad spectrum of viruses, including several flaviviruses. Here we describe a novel antiviral mechanism employed by viperin against two prominent flaviviruses, tick-borne encephalitis virus (TBEV) and Zika virus (ZIKV). Viperin was found to interact and colocalize with the structural proteins premembrane (prM) and envelope (E) of TBEV, as well as with nonstructural (NS) proteins NS2A, NS2B, and NS3. Interestingly, viperin expression reduced the NS3 protein level, and the stability of the other interacting viral proteins, but only in the presence of NS3. We also found that although viperin interacted with NS3 of mosquito-borne flaviviruses (ZIKV, Japanese encephalitis virus, and yellow fever virus), only ZIKV was sensitive to the antiviral effect of viperin. This sensitivity correlated with viperin's ability to induce proteasome-dependent degradation of NS3. ZIKV and TBEV replication was rescued completely when NS3 was overexpressed, suggesting that the viral NS3 is the specific target of viperin. In summary, we present here a novel antiviral mechanism of viperin that is selective for specific viruses in the genus Flavivirus, affording the possible availability of new drug targets that can be used for therapeutic intervention.IMPORTANCE Flaviviruses are a group of enveloped RNA viruses that cause severe diseases in humans and animals worldwide, but no antiviral treatment is yet available. Viperin, a host protein produced in response to infection, effectively restricts the replication of several flaviviruses, but the exact molecular mechanisms have not been elucidated. Here we have identified a novel mechanism employed by viperin to inhibit the replication of two flaviviruses: tick-borne encephalitis virus (TBEV) and Zika virus (ZIKV). Viperin induced selective degradation via the proteasome of TBEV and ZIKV non-structural 3 (NS3) protein, which is involved in several steps of the viral life cycle. Furthermore, viperin also reduced the stability of several other viral proteins in a NS3-dependent manner, suggesting a central role of NS3 in viperin's antiflavivirus activity. Taking the results together, our work shows important similarities and differences among the members of the genus Flavivirus and could lead to the possibility of therapeutic intervention.
|
|
