| 81. |
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| 82. |
- Berglund, Lisa, et al.
(författare)
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A genecentric Human Protein Atlas for expression profiles based on antibodies
- 2008
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 7:10, s. 2019-2027
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Forskningsöversikt (refereegranskat)abstract
- An attractive path forward in proteomics is to experimentally annotate the human protein complement of the genome in a genecentric manner. Using antibodies, it might be possible to design protein-specific probes for a representative protein from every protein-coding gene and to subsequently use the antibodies for systematical analysis of cellular distribution and subcellular localization of proteins in normal and disease tissues. A new version (4.0) of the Human Protein Atlas has been developed in a genecentric manner with the inclusion of all human genes and splice variants predicted from genome efforts together with a visualization of each protein with characteristics such as predicted membrane regions, signal peptide, and protein domains and new plots showing the uniqueness (sequence similarity) of every fraction of each protein toward all other human proteins. The new version is based on tissue profiles generated from 6120 antibodies with more than five million immunohistochemistry-based images covering 5067 human genes, corresponding to approximately 25% of the human genome. Version 4.0 includes a putative list of members in various protein classes, both functional classes, such as kinases, transcription factors, G-protein-coupled receptors, etc., and project-related classes, such as candidate genes for cancer or cardiovascular diseases. The exact antigen sequence for the internally generated antibodies has also been released together with a visualization of the application-specific validation performed for each antibody, including a protein array assay, Western blot analysis, immunohistochemistry, and, for a large fraction, immunofluorescence-based confocal microscopy. New search functionalities have been added to allow complex queries regarding protein expression profiles, protein classes, and chromosome location. The new version of the protein atlas thus is a resource for many areas of biomedical research, including protein science and biomarker discovery.
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| 83. |
- Berglund, Lisa, et al.
(författare)
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Generation of validated antibodies towards the human proteome
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Here we show the results from a large effort to generate antibodies towards the human proteome. A high-throughput strategy was developed based on cloning and expression of antigens as recombitant protein epitope signature tags (PrESTs) Affinity purified polyclonal antibodies were generated, followed by validation by protein microarrays, Western blotting and microarray-based immunohistochemistry. PrESTs were selected based on sequence uniqueness relative the proteome and a bioinformatics analysis showed that unique antigens can be found for at least 85% of the proteome using this general strategy. The success rate from antigen selection to validated antibodies was 31%, and from protein to antibody 55%. Interestingly, membrane-bound and soluble proteins performed equally and PrEST lengths between 75 and 125 amino acids were found to give the highest yield of validated antibodies. Multiple antigens were selected for many genes and the results suggest that specific antibodies can be systematically generated to most human proteibs.
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| 84. |
- Björn, Linnea, 1994, et al.
(författare)
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Process-Induced Structures of Injection-Molded High-Density Polyethylene─Combining X-ray Scattering and Finite Element Modeling
- 2024
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Ingår i: ACS Applied Polymer Materials. - 2637-6105. ; 6:8, s. 4852-4864
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Tidskriftsartikel (refereegranskat)abstract
- The success of plastics heavily relies on fast melt processing methods used for large-scale industrial manufacturing, including injection molding. The hierarchical structure of the solid polymer depends on material selection combined with processing conditions, making mechanical properties of the injection molded part difficult to predict. Here we show how scanning small- and wide-angle X-ray scattering, birefringence microscopy, and polarized light optical microscopy can be combined with injection molding simulations to shed light on the correlation between the polymer morphology of high-density polyethylene and processing conditions. The scattering data revealed that the complex layered structure highly depends on the pressure during the holding phase of injection molding. Furthermore, we identified specific work of flow as a main parameter to capture the changes in morphology induced by varying the process settings. Overall, a good agreement was found between experimental data and the computational simulations, suggesting that computational simulations can be further used to predict the multiphase morphology of injection molded parts.
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| 85. |
- Björn, Linnea, 1994, et al.
(författare)
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Scanning Small-Angle X-ray Scattering of Injection-Molded Polymers: Anisotropic Structure and Mechanical Properties of Low-Density Polyethylene
- 2023
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Ingår i: ACS Applied Polymer Materials. - 2637-6105. ; 5:8, s. 6429-6440
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Tidskriftsartikel (refereegranskat)abstract
- Injection molding is known to create a layered anisotropicmorphologyacross the sample thickness due to varying shear and cooling ratesduring the manufacturing process. In this study, scanning small-angleX-ray scattering was used to visualize and quantify the distributionof hierarchical structures present in injection-molded parts of low-densitypolyethylene (LDPE) with varying viscosities. By combining scatteringdata with results from injection molding simulations and tensile testing,we find that oriented shish-kebab structures, as well as elongatedspherulite structures consisting of semicrystalline ellipsoids, contributeto high ultimate tensile strength along the flow direction. Furthermore,we show that a higher degree of orientation is found close to theinjection gate and in LDPE with higher viscosity, consequently fromelevated shear and cooling rates present during the injection moldingprocess.
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| 86. |
- Bodin, Per, et al.
(författare)
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Guidance, navigation, and control experiments on the PRISMA in-orbit test bed
- 2007
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Ingår i: 58th International Astronautical Congress, IAC-07-C1. - 9781605601502 ; , s. 4461-4470
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Konferensbidrag (refereegranskat)abstract
- PRISMA will demonstrate Guidance, Navigation and Control strategies for advanced autonomous formation flying and rendezvous. The Swedish Space Corporation (SSC) is the prime contractor for the project which is funded by the Swedish National Space Board (SNSB). The project is further supported by the German Aerospace Center (DLR), the Technical University of Denmark (DTU), and the French Space Agency (CNES). PRISMA consists of two spacecraft: MAIN and TARGET. The MAIN satellite is 3-axis stabilized and has full 3D delta-V maneuverability that is independent of the spacecraft's attitude. The TARGET satellite has a simplified, yet 3-axis stabilizing, magnetic attitude control system and no orbit maneuver capability. This paper presents the PRISMA Guidance, Navigation, and Control (GNC) subsystem. The paper gives a mission summary and an overview of the GNC subsystem with its hardware and software configuration. It also explains how the orbit control functions contain advanced fuel optimal Model Predictive Control (MPC). It is shown how the GNC software is developed using model based automatic coding technology implemented with Matlab/Simulink. The paper then summarizes the different GNC experiments to be performed by SSC. Finally, an overview of the test approach for the subsystem is given.
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| 87. |
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| 88. |
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| 89. |
- Bolin, Sara, 1988-, et al.
(författare)
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Combined BET bromodomain and CDK2 inhibition in MYC-driven medulloblastoma
- 2018
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Ingår i: Oncogene. - : Nature Publishing Group. - 0950-9232 .- 1476-5594. ; 37:21, s. 2850-2862
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Tidskriftsartikel (refereegranskat)abstract
- Medulloblastoma (MB) is the most common malignant brain tumor in children. MYC genes are frequently amplified and correlate with poor prognosis in MB. BET bromodomains recognize acetylated lysine residues and often promote and maintain MYC transcription. Certain cyclin-dependent kinases (CDKs) are further known to support MYC stabilization in tumor cells. In this report, MB cells were suppressed by combined targeting of MYC expression and MYC stabilization using BET bromodomain inhibition and CDK2 inhibition, respectively. Such combination treatment worked synergistically and caused cell cycle arrest as well as massive apoptosis. Immediate transcriptional changes from this combined MYC blockade were found using RNA-Seq profiling and showed remarkable similarities to changes in MYC target gene expression when MYCN was turned off with doxycycline in our MYCN-inducible animal model for Group 3 MB. In addition, the combination treatment significantly prolonged survival as compared to single-agent therapy in orthotopically transplanted human Group 3 MB with MYC amplifications. Our data suggest that dual inhibition of CDK2 and BET bromodomains can be a novel treatment approach for suppressing MYC-driven cancer.
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| 90. |
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