| 21. |
- Mou, Tian, et al.
(författare)
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The transcriptome-wide landscape of molecular subtype-specific mRNA expression profiles in acute myeloid leukemia
- 2021
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Ingår i: American Journal of Hematology. - : John Wiley & Sons. - 0361-8609 .- 1096-8652. ; 96:5, s. 580-588
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Tidskriftsartikel (refereegranskat)abstract
- Molecular classification of acute myeloid leukemia (AML) aids prognostic stratification and clinical management. Our aim in this study is to identify transcriptome-wide mRNAs that are specific to each of the molecular subtypes of AML. We analyzed RNA-sequencing data of 955 AML samples from three cohorts, including the BeatAML project, the Cancer Genome Atlas, and a cohort of Swedish patients to provide a comprehensive transcriptome-wide view of subtype-specific mRNA expression. We identified 729 subtype-specific mRNAs, discovered in the BeatAML project and validated in the other two cohorts. Using unique proteomics data, we also validated the presence of subtype-specific mRNAs at the protein level, yielding a rich collection of potential protein-based biomarkers for the AML community. To enable the exploration of subtype-specific mRNA expression by the broader scientific community, we provide an interactive resource to the public.
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| 22. |
- Orre, Lukas Minus, et al.
(författare)
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SubCellBarCode : Proteome-wide Mapping of Protein Localization and Relocalization
- 2019
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Ingår i: Molecular Cell. - : Elsevier BV. - 1097-2765 .- 1097-4164. ; 73:1, s. 166-182.e7
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Tidskriftsartikel (refereegranskat)abstract
- Subcellular localization is a main determinant of protein function; however, a global view of cellular proteome organization remains relatively unexplored. We have developed a robust mass spectrometry-based analysis pipeline to generate a proteome-wide view of subcellular localization for proteins mapping to 12,418 individual genes across five cell lines. Based on more than 83,000 unique classifications and correlation profiling, we investigate the effect of alternative splicing and protein domains on localization, complex member co-localization, cell-type-specific localization, as well as protein relocalization after growth factor inhibition. Our analysis provides information about the cellular architecture and complexity of the spatial organization of the proteome; we show that the majority of proteins have a single main subcellular location, that alternative splicing rarely affects subcellular location, and that cell types are best distinguished by expression of proteins exposed to the surrounding environment. The resource is freely accessible via www.subcellbarcode.org.
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| 23. |
- Pericault, Youen, 1992-, et al.
(författare)
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A novel freeze protection strategy for shallow buried sewer pipes : temperature modelling and field investigation
- 2016
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Ingår i: Proceedings of the 8th International Conference on Sewer Processes & Networks.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The burial of sewer and water pipes below the maximum ground frost depth can be very costly and laborious in regions with cold winters. If a freeze protection measure is applied, the utility lines can be installed in a shallower trench to reduce the excavation needs during construction and maintenance works. One freeze protection measure, so called heat tracing, consist in supplying heat along the pipes. In this work, the use of 4th generation district heating as a heat tracing solution was investigated at a pilot site in Kiruna, Sweden. The influence of the system on sewer and water pipes temperatures was studied experimentally and numerically at a snow-free and snow-covered cross section. The study showed that, under the climatic conditions of the experiment, a heat tracing temperature of 25 ˚C allowed to prevent freezing of the pipes while keeping drinking water pipes in safe temperature range at both cross sections. The other main result was that a finite volume model of the sections was developed and showed a good fitting to the experimental data.
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| 24. |
- Pericault, Youen, 1992-, et al.
(författare)
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A novel freeze protection strategy for shallow buried sewer pipes : temperature modelling and field investigation
- 2017
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Ingår i: Water Science and Technology. - : IWA Publishing. - 0273-1223 .- 1996-9732. ; 76:2, s. 294-301
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Tidskriftsartikel (refereegranskat)abstract
- The burial of sewer and water pipes below the maximum ground frost depth can be very costly and laborious in regions with cold winters. If a freeze protection measure is applied, the utility lines can be installed in a shallower trench to reduce the excavation needs. One freeze protection measure, so called heat tracing, consists in supplying heat along the pipes. In this work, the use of 4th generation district heating as a heat tracing solution was investigated at a pilot site in Kiruna, Sweden. The influence of the system on sewer and water pipe temperatures was studied at a snow-free and snow-covered cross section. To this end, five heat tracing temperatures were tested and the corresponding sewer and water pipe temperatures were measured. The field experiment was also simulated with a two dimensional finite volume model. The study showed that, under the climatic conditions of the experiment, a heat tracing temperature of 25 °C allowed to prevent freezing of the pipes while keeping drinking water pipes in a safe temperature range at both cross sections. The other main result was that the developed finite volume model of the sections showed a good fitting to the experimental data
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| 25. |
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| 26. |
- Ramos Caceres, Cristina, et al.
(författare)
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Data-Center Farming: Exploring the Potential of Industrial Symbiosis in a Subarctic Region
- 2022
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Ingår i: Sustainability. - : MDPI. - 2071-1050. ; 14:5
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Tidskriftsartikel (refereegranskat)abstract
- As our world becomes increasingly digitalized, data centers as operational bases for these technologies lead to a consequent increased release of excess heat into the surrounding environment. This paper studies the challenges and opportunities of industrial symbiosis between data centers’ excess heat and greenhouse farming, specifically utilizing the north of Sweden as a case study region. The region was selected in a bid to tackle the urgent urban issue of self-sufficiency in local food production. A synergetic approach towards engaging stakeholders from different sectors is presented through a mix of qualitative and quantitative methods to facilitate resilient data-center-enabled food production. The paper delivers on possible future solutions on implementing resource efficiency in subarctic regions.
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| 27. |
- Risberg, Daniel, et al.
(författare)
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CFD simulation and evaluation of different heating systems installed in low energy building located in sub-arctic climate
- 2015
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Ingår i: Building and Environment. - : Elsevier BV. - 0360-1323 .- 1873-684X. ; 89, s. 160-169
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Tidskriftsartikel (refereegranskat)abstract
- Computational Fluid Dynamics (CFD) simulations were used to study the indoor climate in a low energy building in northern Sweden. The building’s low heat requirement raise the prospect of using a relatively simple and inexpensive heating system to maintain an acceptable indoor environment, even in the face of extremely low outdoor temperature. To explore the viability of this approach, the indoor climate in the building was studied considering three different heating systems: a floor heating system, air heating through the ventilation system and an air heat pump installation with one fan coil unit. The floor heating system provided the most uniform operative temperature distribution and was the only heating system that fully satisfied the recommendations to achieve tolerable indoor climate set by the Swedish authorities. On the contrary, air heating and the air heat pump created a relatively uneven distribution of air velocities and temperatures, and none of them fulfills the specified recommendations. From the economic point of view, the air heat pump system was cheaper to be installed but produced a less pleasant indoor environment than the other investigated heating systems.
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| 28. |
- Risberg, Daniel, et al.
(författare)
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CFD simulations of the indoor climate of a low energy building in a sub-Arctic climate: an evaluationof different heating systems.
- 2013
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Konferensbidrag (refereegranskat)abstract
- Computational Fluid Dynamics (CFD) simulations were used to study the indoor climate in a low energy building in northern Sweden. The building’s low heat requirements raise the prospect of using relatively simple and inexpensive heating systems to maintain an acceptable indoor environment, even in the face of extremely low outdoor temperatures. To explore the viability of this approach, the indoor temperature and air velocity distribution inside the building were studied assuming that it was fitted with one of four different heating systems: radiators, an underfloor heating system, a pellet stove, and an air/air heat pump. The radiators produced a relatively uniform horizontal temperature distribution throughout the house. The underfloor system provided an even more uniform temperature distribution. In contrast, the heat pump created a relatively uneven internal temperature distribution. Several locations for the pump were considered, all of which had significant drawbacks. The pellet stove produced a more even temperature distribution than the pump but not to the same extent as the underfloor system or the radiators. Overall, point source heating systems cost less to fit and operate over a given period of time but produce a less clement indoor environment than distributed heating systems.
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| 29. |
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| 30. |
- Rykaczewska, Urszula, et al.
(författare)
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PCSK6 Is a Key Protease in the Control of Smooth Muscle Cell Function in Vascular Remodeling
- 2020
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Ingår i: Circulation Research. - : LIPPINCOTT WILLIAMS & WILKINS. - 0009-7330 .- 1524-4571. ; 126:5, s. 571-585
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Tidskriftsartikel (refereegranskat)abstract
- Rationale: PCSKs (Proprotein convertase subtilisins/kexins) are a protease family with unknown functions in vasculature. Previously, we demonstrated PCSK6 upregulation in human atherosclerotic plaques associated with smooth muscle cells (SMCs), inflammation, extracellular matrix remodeling, and mitogens. Objective: Here, we applied a systems biology approach to gain deeper insights into the PCSK6 role in normal and diseased vessel wall. Methods and Results: Genetic analyses revealed association of intronic PCSK6 variant rs1531817 with maximum internal carotid intima-media thickness progression in high-cardiovascular risk subjects. This variant was linked with PCSK6 mRNA expression in healthy aortas and plaques but also with overall plaque SMA+ cell content and pericyte fraction. Increased PCSK6 expression was found in several independent human cohorts comparing atherosclerotic lesions versus healthy arteries, using transcriptomic and proteomic datasets. By immunohistochemistry, PCSK6 was localized to fibrous cap SMA+ cells and neovessels in plaques. In human, rat, and mouse intimal hyperplasia, PCSK6 was expressed by proliferating SMA+ cells and upregulated after 5 days in rat carotid balloon injury model, with positive correlation to PDGFB (platelet-derived growth factor subunit B) and MMP (matrix metalloprotease) 2/MMP14. Here, PCSK6 was shown to colocalize and cointeract with MMP2/MMP14 by in situ proximity ligation assay. Microarrays of carotid arteries from Pcsk6(-/-) versus control mice revealed suppression of contractile SMC markers, extracellular matrix remodeling enzymes, and cytokines/receptors. Pcsk6(-/-) mice showed reduced intimal hyperplasia response upon carotid ligation in vivo, accompanied by decreased MMP14 activation and impaired SMC outgrowth from aortic rings ex vivo. PCSK6 silencing in human SMCs in vitro leads to downregulation of contractile markers and increase in MMP2 expression. Conversely, PCSK6 overexpression increased PDGFBB (platelet-derived growth factor BB)-induced cell proliferation and particularly migration. Conclusions: PCSK6 is a novel protease that induces SMC migration in response to PDGFB, mechanistically via modulation of contractile markers and MMP14 activation. This study establishes PCSK6 as a key regulator of SMC function in vascular remodeling.
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