| 21. |
- Brohlin, Maria, 1966-, et al.
(author)
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Effects of a defined xeno-free medium on the growth and neurotrophic and angiogenic properties of human adult stem cells
- 2017
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In: Cytotherapy. - : ELSEVIER SCI LTD. - 1465-3249 .- 1477-2566. ; 19:5, s. 629-639
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Journal article (peer-reviewed)abstract
- Background. The growth properties and neurotrophic and angiogenic effects of human mesenchymal stromal cells (MSCs) cultured in a defined xeno-free, serum-free medium (MesenCult-XF) were investigated. Methods. Human MSCs from adipose tissue (ASCs) and bone marrow (BMSCs) were cultured in Minimum Essential Medium-alpha (alpha-MEM) containing fetal calf serum or in MesenCult-XF. Proliferation was measured over 10 passages and the colony-forming unit (CFU) assay and expression of cluster of differentiation (CD) surface markers were determined. Neurite outgrowth and angiogenic activity of the MSCs were determined. Results. At early passage, both ASCs and BMSCs showed better proliferation in MesenCult-XF compared with standard a-MEM containing serum. However, CFUs were significantly lower in MesenCult-XF. ASCs cultured in MesenCult-XF continued to expand at faster rates than cells grown in serum. BMSCs showed morphological changes at late passage in MesenCult-XF and stained positive for senescence beta-galactosidase activity. Expression levels of CD73 and CD90 were similar in both cell types under the various culture conditions but CD105 was significantly reduced at passage 10 in MesenCult-XF. In vitro stimulation of the cells enhanced the expression of brain derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF-A) and angiopoietin-1. Stimulated ASCs grown in MesenCult-XF evoked the longest neurite outgrowth in a neuron co-culture model. Stimulated BMSCs grown in MesenCult-XF produced the most extensive network of capillary-like tube structures in an in vitro angiogenesis assay. Conclusions. ASCs and BMSCs exhibit high levels of neurotrophic and angiogenic activity when grown in the defined serum free medium indicating their suitability for treatment of various neurological conditions. However, long-term expansion in MesenCult-XF might be restricted to ASCs.
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| 22. |
- Brohlin, Maria
(author)
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Mesenchymal stem cells for repair of the peripheral and central nervous system
- 2011
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Doctoral thesis (other academic/artistic)abstract
- Bone marrow-derived mesenchymal stem cells (MSC) have been shown to provide neuroprotection after transplantation into the injured nervous system. The present thesis investigates whether adult human and rat MSC differentiated along a Schwann cell lineage could increase their expression of neurotrophic factors and promote regeneration after transplantation into the injured peripheral nerve and spinal cord. Human and rat mesenchymal stem cells (hMSC and rMSC) expressed characteristic stem cell surface markers, mRNA transcripts for different neurotrophic factors and demonstrated multi-lineage differentiation potential. Following treatment with a cocktail of growth factors, the hMSC and rMSC expressed typical Schwann cells markers at both the transcriptional and translational level and significantly increased production of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF). Age and time in culture are of relevance for clinical settings and growth-promoting effects of hMSC from young donors (16-18 years) and old donors (67-75 years) were compared. Undifferentiated hMSC from both young and old donors increased total neurite length of cultured dorsal root ganglion (DRG) neurons. Differentiation of hMSC from the young donors, but not the eldery donors, further enhanced the neurite outgrowth. Undifferentiated hMSC were cultured for eleven weeks in order to examine the effect of in vitro expansion time on neurite outgrowth. hMSC from the young donors maintained their proliferation rate and their ability to enhance neurite outgrowth from DRG neurons. Using a sciatic nerve injury model, a 10mm gap was bridged with either an empty tubular fibrin glue conduit, or conduits containing hMSC, with and without cyclosporine treatment. Cells were labeled with PKH26 prior to transplantation. At 3 weeks after injury the conduits with cells and immunosuppression increased regeneration compared with an empty conduit. PKH26 labeled human cells survived in the rat model and the inflammatory reaction could be suppressed by cyclosporine. After cervical C4 hemisection, BrdU/GFP-labeled rMSC were injected into the lateral funiculus rostral and caudal to the spinal cord lesion site. Spinal cords were analyzed 2-8 weeks after transplantation. Transplanted MSC remained at the injection sites and in the trauma zone for several weeks and were often associated with numerous neurofilament-positive axons. Transplanted rMSC induced up-regulation of vascular endothelial growth factor in spinal cord tissue rostral to the injury site, but did not affect expression of brain-derived neurotrophic factor. Although rMSC provided neuroprotection for rubrospinal neurons and significantly attenuated astroglial and microglial reaction, cell transplantation caused aberrant sprouting of calcitonin gene-related peptide immunostained sensory axons in the dorsal horn. In summary these results demonstrate that both rat and human MSC can be differentiated towards the glial cell lineage, and show functional characteristics similar to Schwann cells. hMSC from the young donors represent a more favorable source for neurotransplantation since they maintain proliferation rate and preserve their growth-promoting effects in long-term cultures. The data also suggest that differentiated MSC increase expression of neurotrophic factors and support regeneration after peripheral nerve and spinal cord injury.
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| 23. |
- Carelli, Maria Grazia, et al.
(author)
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Broadening the TP Profile : Future Negative Time Perspective
- 2015. - 1
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In: Time Perspective Theory; Review, Research and Application. - New York : Springer-Verlag New York. - 9783319073675 ; , s. 87-97
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Book chapter (peer-reviewed)abstract
- The importance of the future as an arena for planning, self-regulation and achievement has been of considerable interest in past research. The majority of this research suggests that future-oriented thinking has considerable benefits for psychological adjustment and wellbeing. The future is nevertheless not only a temporal space for goal-setting and positive expectations, it may also be associated with fear, uncertainty and anxiety, which may ultimately have detrimental effects on both mental and physical health. Here we present the outline for the Swedish ZTPI (S-ZTPI) which extends the original ZTPI by separating the Future dimension into two sub-factors: The Future Positive scale and the Future Negative scale. We argue that separating the future into two separate dimensions thus comprehending both a positive and a negative valence of the future, adds important information regarding association between future time perspective and subjective well-being.
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| 24. |
- Carelli, Maria Grazia, 1959-, et al.
(author)
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Time out of mind : temporal perspective in adults with ADHD
- 2012
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In: Journal of Attention Disorders. - : SAGE. - 1087-0547 .- 1557-1246. ; 16:6, s. 460-466
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Journal article (peer-reviewed)abstract
- Objective: ADHD is often associated with difficulties in planning and time management. In this study, the authors examined the hypothesis that these functional problems in ADHD reflect systematic biases in temporal orientation.Method: To test this hypothesis, adults with ADHD (n = 30) and healthy controls (n = 60) completed the Swedish version of the Zimbardo Time Perspective Inventory (S-ZTPI).Results: Although a majority of the ADHD participants were tested under stimulant medication, they showed significant differences in all the six subscales of the S-ZTPI. Logistic regression analysis, with age, education, depression, and response inhibition as covariates, showed that the Future Positive Scale was the primary predictor of ADHD status.Conclusion: These findings suggest that ADHD is associated with systematic biases in habitual time orientation and that these differences may contribute to functional problems in ADHD.
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| 25. |
- Carlson, Maja, et al.
(author)
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Samarbetsmöjligheter kring och kvalitetssäkring av e-lärande
- 2018
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In: Om samverkan, mångfald och mellanmänskliga möten : Proceedings från Lunds Universitets Pedagogiska Utvecklingskonferens 2017 - Proceedings från Lunds Universitets Pedagogiska Utvecklingskonferens 2017. - 9789188473974 ; , s. 48-54
-
Conference paper (peer-reviewed)
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| 26. |
- Ceynowa, Dylan J., et al.
(author)
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Morning Glory Disc Anomaly in childhood - a population-based study
- 2015
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In: Acta Ophthalmologica. - : Wiley. - 1755-375X .- 1755-3768. ; 93:7, s. 626-634
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Journal article (peer-reviewed)abstract
- Purpose: To report prevalence, ocular characteristics and coexisting neurological, behavioural, somatic and neuroradiological abnormalities in children and adolescents with morning glory disc anomaly (MGDA).Methods: In a cross-sectional population-based study, 12 patients with MGDA, aged 2-20years, were identified. All 12 agreed to ophthalmological assessments including visual functions, refraction, fundus photography, optical coherence tomography (OCT) and ocular motor score (OMS). Neurological examinations and behavioural/developmental screening were carried out. Data from previous or new neuroradiological investigations were collected.Results: The prevalence of MGDA was 2.6/100000. MGDA was unilateral in 11/12 patients with a best-corrected visual acuity (BCVA) in the MGDA eye ranging from hand motion to 0.65 (median 0.06). Severe microphthalmus prevented unilaterality to be determined in one adolescent. All patients had a binocular BCVA of 0.5. OMS showed abnormalities in pupil response, vestibulo-ocular reflex, stereo visual acuity, strabismus and convergence. OCT revealed peripapillary or macular oedema in 5/8 patients and foveal aplasia in 3/8 patients. Three patients had extensive capillary hemangiomas, of which one had PHACES syndrome and one had additional cerebrovascular anomalies and corpus callosum agenesis. Neuroradiology showed craniovascular anomalies in two patients. Neurology was mostly normal. Behavioural/developmental screening showed attention deficit hyperactivity disorder in one patient.Conclusions: The prevalence data, previously not reported, of morning glory disc anomaly was 2.6/100 000. Coexisting retinal peripapillary or macular oedema was common, as were cerebral abnormalities and/or cutaneous vascular malformations. The associated findings may not be discovered through routine ophthalmological examination why OCT and neuroimaging are called for.
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| 27. |
- Dahl, S., et al.
(author)
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High prevalence of pituitary hormone deficiency in both unilateral and bilateral optic nerve hypoplasia
- 2019
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In: Acta Paediatrica. - : Wiley. - 0803-5253 .- 1651-2227. ; 108:9, s. 1677-1685
-
Journal article (peer-reviewed)abstract
- Aim This study examined the prevalence of neurological impairment and pituitary hormone deficiency (PHD) in patients with unilateral and bilateral optic nerve hypoplasia (ONH). Methods A population-based cross-sectional cohort study of 65 patients (51% female) with ONH was conducted in Stockholm. Of these were 35 bilateral and 30 unilateral. The patients were below 20 years of age, living in Stockholm in December 2009 and found through database searching. The median age at the analysis of the results in January 2018 was 16.1 years (range 8.1-27.5 years). Neurological assessments and blood sampling were conducted, neuroradiology was reviewed and growth curves were analysed. Diagnoses of PHDs were based on clinical and biochemical evidence of hormone deficiency. Results Neurological impairments were identified in 47% of the patients and impairments in gross and fine motor function were more prevalent in bilateral ONH (p < 0.001). In addition, 9% had cerebral palsy and 14% had epilepsy. The prevalence of PHD was 29 and 19% had multiple PHD. Conclusion Children with ONH had a high risk of neurological impairment, especially in bilateral disease. Both unilateral and bilateral ONH signified an increased prevalence of PHD and all these children should be endocrinologically followed up until completed puberty.
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| 28. |
- Ek, Lena, et al.
(author)
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Decline in executive functions and speed in suspected low-grade gliomas : A 3-year follow-up of a clinical cohort
- 2018
-
In: Applied Neuropsychology. Adult. - : Informa UK Limited. - 2327-9095 .- 2327-9109. ; 25:4, s. 376-384
-
Journal article (peer-reviewed)abstract
- Changes over time in information processing speed and executive functions (EFs) were studied in patients with suspected low-grade gliomas (LGG) 3 years after diagnosis. Using a person-oriented approach, the study aimed at focusing solely on two cognitive domains known to be significant in the understanding of the impact of white matter diseases. The Barkley’s hybrid model of EFs was used as a theoretical framework for the evaluation of EFs. The majority of the patients showed a decline in at least one of these two cognitive domains indicating that the progress of diffuse brain injury cannot be neglected in understanding neuropsychological changes over time in patients with LGG. In our sample, higher age and radiological signs of radiotherapy-induced brain atrophy were seen in patients with a decline in both domains.
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| 29. |
- Ek, Lena, 1961-, et al.
(author)
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Early cognitive impairment in a subset of patients with presumed low-grade glioma
- 2010
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In: Neurocase. - : Informa UK Limited. - 1355-4794 .- 1465-3656. ; 16:6, s. 503-511
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Journal article (peer-reviewed)abstract
- We investigated the presence of cognitive impairment, in adults with presumed low-grade glioma at early stage of disease prior to major treatments, in relation to neurological symptoms and radiological characteristics of the tumour. Sixteen patients were evaluated. A subset of patients was identified with clearly impaired cognition. Patients with cognitive impairment often had large tumours in the left frontal lobe, were relatively young, and most of them were males. We conclude that cognitive dysfunction may be present already at early stage of disease, and that early identification of patients at risk is warranted.
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| 30. |
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Ett bibliotek i takt med tiden : Linköpings universitetsbibliotek under 50 år
- 2022
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Artistic workabstract
- Utställningen Ett bibliotek i takt med tiden: Linköpings universitetsbibliotek under 50 år skildrade bibliotekets historia och biblioteksteknikens utveckling från 1970-talet till idag.Utställningen fokuserade på olika teman som varit signifikativa för Linköpings universitetsbibliotek, som etablerades 1969 som Linköpings högskolas bibliotek. Dessa teman var den så kallade Linköpingsmodellen och dess kvartersbibliotek, försöksverksamheten med bibliotekssystemet Libris i början av 1970-talet, de första databaserna för att söka vetenskapliga publikationer, högskolereformen 1977 och dess betydelse för universitetsbiblioteket, tillkomsten av Hälsouniversitetets bibliotek 1986, datoriseringen under 1980-talet, användarundervisningens utveckling, tillkomsten av det elektroniska förlaget LiU E-Press 1996, skapande av Campus Norrköpings bibliotek 1998, den tilltagande digitaliseringen under 2000-talet, bibliometrins ökande betydelse samt utvecklingen under 2010-talet med bland annat byte av klassifikationssystem, flyttar och sammanslagningar av bibliotek.
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