| 61. |
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| 62. |
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| 63. |
- Wickström, David, et al.
(författare)
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Characterization of the Consequences of YidC Depletion on the Inner Membrane Proteome of E. coli Using 2D Blue Native/SDS-PAGE
- 2011
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Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836 .- 1089-8638. ; 409:2, s. 124-135
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Tidskriftsartikel (refereegranskat)abstract
- In the bacterium Escherichia coli, the essential inner membrane protein (IMP) YidC assists in the biogenesis of IMPs and IMP complexes. Our current ideas about the function of YidC are based on targeted approaches using only a handful of model IMPs. Proteome-wide approaches are required to further our understanding of the significance of YidC and to find new YidC substrates. Here, using two-dimensional blue native/SDS-PAGE methodology that is suitable for comparative analysis, we have characterized the consequences of YidC depletion for the steady-state levels and oligomeric state of the constituents of the inner membrane proteome. Our analysis showed that (i) YidC depletion reduces the levels of a variety of complexes without changing their composition, (ii) the levels of IMPs containing only soluble domains smaller than 100 amino acids are likely to be reduced upon YidC depletion, whereas the levels of IMPs with at least one soluble domain larger than 100 amino acids do not, and (iii) the levels of a number of proteins with established or putative chaperone activity (HflC, HflK, PpiD, OppA, GroEL and DnaK) are strongly increased in the inner membrane fraction upon YidC depletion. In the absence of YidC, these proteins may assist the folding of sizeable soluble domains of IMPs, thereby supporting their folding and oligomeric assembly. In conclusion, our analysis identifies many new IMPs/IMP complexes that depend on YidC for their biogenesis, responses that accompany depletion of YidC and an IMP characteristic that is associated with YidC dependence.
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| 64. |
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| 65. |
- Wijk, Helle, 1958, et al.
(författare)
-
Color discrimination, color naming and color preferences in 80-year olds.
- 1999
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Ingår i: Aging. ; 11:3, s. 176-8
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the present study was to investigate color discrimination, color naming and color preference in a random sample of 80-year-old men and women. Knowledge of color perception in old age can be of value when using color contrast, cues and codes in the environment to promote orientation and function. The color naming test indicated that the colors white, black, yellow, red, blue and green promoted recognition to the highest degree among all subjects. A gender-related difference, in favor of women, occurred in naming five of the mixed colors. Women also used more varied color names than men. Color discrimination was easier in the red and yellow area than in the blue and green area. This result correlates positively with visual function on far sight, and negatively with diagnosis of a cataract. The preference order for seven colors put blue, green and red at the top, and brown at the bottom, hence agreeing with earlier studies, and indicating that the preference order for colors remains relatively stable also in old age. This result should be considered when designing environments for old people. PMID: 10476313 [PubMed - indexed for MEDLINE]
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| 66. |
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| 67. |
- Wijk, Helle, 1958, et al.
(författare)
-
Colour discrimination, colour naming and colour preferences among individuals with Alzheimer's disease.
- 1999
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Ingår i: Int J Geriatr Psychiatry. ; 14:12, s. 1000-5
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To study the ability of colour naming, colour discrimination and colour preference in Alzheimer's disease (AD). DESIGN: Descriptive, consecutive sample. PARTICIPANTS: Fifty subjects >65 years with AD. INTERVENTIONS: Testing colour discrimination, colour naming and colour preferences. MAIN OUTCOME MEASURES: Ability to detect colour differences in the yellow, red, blue and green areas, ability to assign a name to 22 colour samples, ability to rank seven colours in order of preference. MAIN RESULTS: Discrimination ability was significantly better in the yellow and red area and for lightness variations. Cognitive decline had a significant impact on naming mixed colours and using elaborate colour names. Severity of dementia did not affect the preference rank order of colours. CONCLUSIONS: Ability to discriminate is affected in AD, with most errors in the blue and green area. Naming colours shows a cognitive decline. Preferences for colour are stable despite the disease. Copyright 1999 John Wiley & Sons, Ltd. PMID: 10607966 [PubMed - indexed for MEDLINE]
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| 68. |
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| 69. |
- Wijk, Helle, 1958, et al.
(författare)
-
Colour perception among the very elderly related to visual and cognitive function.
- 2002
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Ingår i: Scand J Caring Sci.. ; 16:1, s. 91-102
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Tidskriftsartikel (refereegranskat)abstract
- Colour perception was studied among a representative sample of 95-year olds and compared with previously examined 80-year olds and a group with Alzheimer's disease (AD), mean age of 80 years. The 95-year olds' results were on a significantly lower level than the other two groups but showed a similar pattern as to colour -discrimination, -naming, -preferences and colour/form recognition. Visual function among 95-year olds had only minor influence on their result in contrast to cognitive function which had a more profound impact. Most interestingly though, the subjects with Alzheimer's diagnosis, younger than the 95-year olds but with a conclusive diagnosis of dementia, performed on an overall higher level than the 95-year olds. This advantage for the Alzheimer group remained even when compared with a sub sample of the 95-year olds with possible dementia diagnosis excluded. On the other hand, only including 95-year olds with a Mini Mental Examination score of >26 in comparison with the Alzheimer group made the differences between the two groups disappear. It is suggested that colour perception as measured in this study is well preserved throughout life. The overall lower colour perception ability in 95-year olds compared with both 80-year olds and subjects with AD may be an expression of the complexity of very high age rather than any isolated concomitant factor. PMID: 11985755 [PubMed - indexed for MEDLINE
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| 70. |
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