| 1031. |
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| 1032. |
- Nugent, Rebecca L., et al.
(författare)
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Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation
- 2010
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Ingår i: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- Background: Histone acetyltransferase enzymes (HATs) are implicated in egulation of transcription. HATs from different families may overlap in arget and substrate specificity. esults: We isolated the elp3(+) gene encoding the histone cetyltransferase subunit of the Elongator complex in fission yeast and haracterized the phenotype of an Delta elp3 mutant. We examined genetic nteractions between Delta elp3 and two other HAT mutants, Delta mst2 nd Delta gcn5 and used whole genome microarray analysis to analyze heir effects on gene expression. onclusions: Comparison of phenotypes and expression profiles in single, ouble and triple mutants indicate that these HAT enzymes have verlapping functions. Consistent with this, overlapping specificity in istone H3 acetylation is observed. However, there is no evidence for verlap with another HAT enzyme, encoded by the essential mst1(+) gene.
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| 1033. |
- Nygren, E, et al.
(författare)
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Helping clinicians to find data and avoid delays
- 1998
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Ingår i: LANCET. - : LANCET LTD. - 0140-6736. ; 352:9138, s. 1462-1466
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Tidskriftsartikel (refereegranskat)abstract
- One major criticism of paper medical records is the time and effort required to find data items or to gain an overview. Computerisation does not necessarily help. To help clinicians find data faster and with less effort, everyone designing and writing in
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| 1034. |
- O'Dushlaine, Colm, et al.
(författare)
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Genes predict village of origin in rural Europe
- 2010
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Ingår i: European Journal of Human Genetics. - : Springer Science and Business Media LLC. - 1018-4813 .- 1476-5438. ; 18:11, s. 1269-1270
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Tidskriftsartikel (refereegranskat)abstract
- The genetic structure of human populations is important in population genetics, forensics and medicine. Using genome-wide scans and individuals with all four grandparents born in the same settlement, we here demonstrate remarkable geographical structure across 8-30 km in three different parts of rural Europe. After excluding close kin and inbreeding, village of origin could still be predicted correctly on the basis of genetic data for 89-100% of individuals.
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| 1035. |
- Oksinska, Malgorzata P., et al.
(författare)
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Colonization of wheat seedlings (Triticum aestivum L.) by strains of Pseudomonas spp. with respect to their nutrient utilization profiles
- 2011
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Ingår i: European journal of soil biology. - : Elsevier BV. - 1164-5563 .- 1778-3615. ; 47:6, s. 364-373
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Tidskriftsartikel (refereegranskat)abstract
- Nutrients that leach out from roots constitute a major source of food for root-colonizing bacteria. The role of specific nutrients in this interaction is unclear. The aim of the present study was to examine whether colonization ability could be attributed to specific nutrient utilization profiles. Twenty fluorescent pseudomonads were tested for colonization ability of 48-h-old wheat seedlings. Analyses of RFLPs of amplified 165 rRNA gene and of BIOLOG GN2 data demonstrated that colonization ability did not associate with any particular RFLP or metabolic group. The best colonizers PPS96, PSR2, PSR21, good colonizer PSR6 and four of ineffective colonizers were identified through 16S sequence analysis as Pseudomonas reactans. The best and good colonizers distinguished themselves from the less efficient colonizers by specifically utilizing: p-hydroxyphenylacetic acid, bromosuccinic acid, benzoic acid, methyl pyruvate. N-acetyl-D-glucosamine, D-trehalose and adonitol. However, there was no specific sole nutrient utilization profile that predicted excellent root colonization ability of wheat, since the best colonizers did not have identical profiles. This work indicates that strains of P. reactans are present in the rhizosphere of oil seed rape and wheat and that some of them are effective colonizers of wheat roots.
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| 1036. |
- Oksinska, M. P., et al.
(författare)
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Resistance to oxidation products of caffeic acid is important for efficient colonization of wheat seedlings by Pseudomonas proteolytica strain PSR114
- 2013
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Ingår i: Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 66, s. 71-80
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Tidskriftsartikel (refereegranskat)abstract
- The interrelationships between plants and rhizosphere bacteria are strongly dependent on the quality and quantity of root exudates. The ability to colonize roots is crucial for pseudomonads to function as biological control agents of root- and soil-borne pathogenic microbes. The multiplication of rhizosphere bacteria is restricted in the presence of simple phenolic compounds, which are components of the resistance mechanisms of plants to pathogens. Caffeic acid is a phenolic compound, which is commonly found in wheat tissues. It is prone to oxidation into o-quinones, which are toxic to microorganisms. The aim of the present study was to determine whether the ability of microorganisms to resist caffeic acid and its oxidation products could play a role in the early colonization of wheat seedlings. Among the fluorescent pseudomonads that we have studied, strain PSR114 is one of the most efficient colonizers of wheat seedlings during the first 48 h after seed germination, and it is particularly resistant to products resulting from the spontaneous oxidation of caffeic acid. This strain was isolated from the rhizosphere of oilseed rape and identified as being closely related to Pseudomonas proteolytica through the analysis of 16S rRNA and rpoB gene sequences. At pH 7.0, this strain grew intensively in the presence of 1.50 mg mL−1 of caffeic acid. Its multiplication was partially reduced in the presence of oxidized caffeic acid at concentrations above 0.21 mg mL−1, and completely inhibited at concentrations above 0.38 mg mL−1. A Tn5 transposon mutant of PSR114 had lower level of resistance to the oxidation products of caffeic acid, as well as reduced capacity to colonize wheat seedlings when compared to the wild type strain. This work demonstrates that resistance to oxidation products of caffeic acid can be important for successful bacterial colonization of wheat seedlings.
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| 1037. |
- Ongena, J., et al.
(författare)
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Synergetic heating of D-NBI ions in the vicinity of the mode conversion layer in H-D plasmas in JET with the ITER like wall.
- 2017
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Ingår i: EPJ Web of Conferences. - : EDP Sciences. - 2100-014X.
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Konferensbidrag (refereegranskat)abstract
- This paper discusses the extension of the 'three-ion' species ICRF technique for heating mixture plasmas using fast injected NBI ions as resonant 'third' species. In this scenario the ICRF power is absorbed by the fast beam ions in the vicinity of the mode conversion layer where the left-hand polarized RF electric field E+ is strongly enhanced. The ions in the beam velocity distribution that have a Doppler-shifted resonance close to the mode conversion layer efficiently absorb RF power and undergo acceleration. We show first experimental observations of ICRF heating of D-NBI ions in H-D plasmas in JET with the ITER-like wall. In agreement with theoretical predictions and numerical modelling, acceleration of the D-NBI ions in this D-(DNBI)-H scenario is confirmed by several fast-ion measurements. An extension of the heating scheme discussed here is acceleration of T-NBI and D-NBI ions in D-T plasmas, offering the potential to further boost the Q-value in future D-T campaigns in JET.
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| 1038. |
- O'Seaghdha, Conall M., et al.
(författare)
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Meta-Analysis of Genome-Wide Association Studies Identifies Six New Loci for Serum Calcium Concentrations
- 2013
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Ingår i: PLOS Genetics. - : Public Library of Science (PLoS). - 1553-7390 .- 1553-7404. ; 9:9, s. e1003796-
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Tidskriftsartikel (refereegranskat)abstract
- Calcium is vital to the normal functioning of multiple organ systems and its serum concentration is tightly regulated. Apart from CASR, the genes associated with serum calcium are largely unknown. We conducted a genome-wide association meta-analysis of 39,400 individuals from 17 population-based cohorts and investigated the 14 most strongly associated loci in <= 21,679 additional individuals. Seven loci (six new regions) in association with serum calcium were identified and replicated. Rs1570669 near CYP24A1 (P = 9.1E-12), rs10491003 upstream of GATA3 (P = 4.8E-09) and rs7481584 in CARS (P = 1.2E-10) implicate regions involved in Mendelian calcemic disorders: Rs1550532 in DGKD (P = 8.2E-11), also associated with bone density, and rs7336933 near DGKH/KIAA0564 (P = 9.1E-10) are near genes that encode distinct isoforms of diacylglycerol kinase. Rs780094 is in GCKR. We characterized the expression of these genes in gut, kidney, and bone, and demonstrate modulation of gene expression in bone in response to dietary calcium in mice. Our results shed new light on the genetics of calcium homeostasis.
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| 1039. |
- Ou, Anna H., et al.
(författare)
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Lithium response in bipolar disorder is associated with focal adhesion and PI3K-Akt networks: a multi-omics replication study
- 2024
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Ingår i: TRANSLATIONAL PSYCHIATRY. - 2158-3188. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- Lithium is the gold standard treatment for bipolar disorder (BD). However, its mechanism of action is incompletely understood, and prediction of treatment outcomes is limited. In our previous multi-omics study of the Pharmacogenomics of Bipolar Disorder (PGBD) sample combining transcriptomic and genomic data, we found that focal adhesion, the extracellular matrix (ECM), and PI3K-Akt signaling networks were associated with response to lithium. In this study, we replicated the results of our previous study using network propagation methods in a genome-wide association study of an independent sample of 2039 patients from the International Consortium on Lithium Genetics (ConLiGen) study. We identified functional enrichment in focal adhesion and PI3K-Akt pathways, but we did not find an association with the ECM pathway. Our results suggest that deficits in the neuronal growth cone and PI3K-Akt signaling, but not in ECM proteins, may influence response to lithium in BD.
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| 1040. |
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