| 2871. |
- Hu, W, et al.
(författare)
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Iterative Strategy Analyze During Agile Development
- 2012
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Ingår i: Weidianzixue yu jisuanji [Microelectronics & Computer]. - 1000-7180. ; 29:5, s. 165-169
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- In this paper, We used characteristic of the Unified Modeling Language （UML） use case model in describing the function of the system＇ s and the iteration sequence of agile development process to obtain a new iteration sequence based on the risk an value of the various use cases in UML use case diagrams. An obvious advantage of doing so is to ensure high-risk and high-value use cases will be first developed, so that the use cases will be repeatedly tested in the next iteration, thereby increasing overall system reliability.
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| 2872. |
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| 2873. |
- Hu, W., et al.
(författare)
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Requires analysis based on software maintainability
- 2014
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Ingår i: ICRMS 2014 - Proceedings of 2014 10th International Conference on Reliability, Maintainability and Safety. - 9781479919925 ; , s. 354-357
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Konferensbidrag (refereegranskat)abstract
- Requirements analysis is the most important phase of the software life cycle process. Some studies have shown the most faults of software are from the requirements phase. Therefore, the quality of Software Requirements Specification has become the key to project success, which correctness, consistency, no ambiguity of software requirements specification is more important. This paper presents a classification method based on natural language processing techniques and grey similar correlation. The first step of this method is that keywords refined from various functional requirements through segmentation of natural language processing, thus made up of heavy weight vector based on the weight of functional requirements, such a functional requirement corresponds to a weight vector. The second step is that the related technology of grey system is used to compute grey correlation coefficient between two weight vectors, in order to construct a correlation matrix. Finally, the appropriate statistical tools are used to classify functional requirements statements. The clustering results based on this method can provide work guidance for requirement analysts, software developers, software testers, software maintenance.
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| 2874. |
- Hu, W, et al.
(författare)
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VDM++ Formalization of UML Class Diagram
- 2012
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Ingår i: Weidianzixue yu jisuanji [Microelectronics & Computer]. - 1000-7180. ; 29:6, s. 104-107
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The purpose of this paper is to use the most widely used formal language-VDM ＋ ＋ that has characteristics of accuracy and consistent in describing syntax and semantic of the system model, combined with VDMTOOLS and Rational Rose tool to transform the various elements of the UML class model into representation of VI）M＋ ＋. This method achieves the various elements contained UML class model about the syntax and semantics checking, and further improves the quality of UML modeling.
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| 2875. |
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| 2876. |
- Hu, XH, et al.
(författare)
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Neogenin suppresses tumor progression and metastasis via inhibiting Merlin/YAP signaling
- 2023
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Ingår i: Cell death discovery. - : Springer Science and Business Media LLC. - 2058-7716. ; 9:1, s. 47-
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Tidskriftsartikel (refereegranskat)abstract
- From in situ growth to invasive dissemination is the most lethal attribute of various tumor types. This transition is majorly mediated by the dynamic interplay between two cancer hallmarks, EMT and cell cycle. In this study, we applied nonlinear association analysis in 33 cancer types and found that most signaling receptors simultaneously associating with EMT and cell cycle are potential tumor suppressors. Here we find that a top co-associated receptor, Neogenin (NEO1), inhibits colorectal cancer (CRC) and Glioma in situ growth and metastasis by forming a complex with Merlin (NF2), and subsequent simultaneous promoting the phosphorylation of YAP. Furthermore, Neogenin protein level is associated with good prognosis and correlates with Merlin status in CRC and Glioma. Collectively, our results define Neogenin as a tumor suppressor in CRC and Glioma that acts by restricting oncogenic signaling by the Merlin-YAP pathway, and suggest Neogenin as a candidate therapeutic agent for CRC and Glioma.
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| 2877. |
- Hu, Yang, 1989-, et al.
(författare)
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In Vivo Quantitative Understanding of PEGylated Liposome’s Influence on Brain Delivery of Diphenhydramine
- 2018
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Ingår i: Molecular Pharmaceutics. - : American Chemical Society (ACS). - 1543-8384 .- 1543-8392. ; 15:12, s. 5493-5500
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Tidskriftsartikel (refereegranskat)abstract
- Despite the promising features of liposomes as brain drug delivery vehicles, it remains uncertain how they influence the brain uptake in vivo. In order to gain a better fundamental understanding of the interaction between liposomes and the blood–brain barrier (BBB), it is indispensable to test if liposomes affect drugs with different BBB transport properties (active influx or efflux) differently. The aim of this study was to quantitatively evaluate how PEGylated (PEG) liposomes influence brain delivery of diphenhydramine (DPH), a drug with active influx at the BBB, in rats. The brain uptake of DPH after 30 min intravenous infusion of free DPH, PEG liposomal DPH, or free DPH + empty PEG liposomes was compared by determining the unbound DPH concentrations in brain interstitial fluid and plasma with microdialysis. Regular blood samples were taken to measure total DPH concentrations in plasma. Free DPH was actively taken up into the brain time-dependently, with higher uptake at early time points followed by an unbound brain-to-plasma exposure ratio (Kp,uu) of 3.0. The encapsulation in PEG liposomes significantly decreased brain uptake of DPH, with a reduction of Kp,uu to 1.5 (p < 0.05). When empty PEG liposomes were coadministered with free drug, DPH brain uptake had a tendency to decrease (Kp,uu 2.3), and DPH was found to bind to the liposomes. This study showed that PEG liposomes decreased the brain delivery of DPH in a complex manner, contributing to the understanding of the intricate interactions between drug, liposomes, and the BBB.
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| 2878. |
- Hu, Yang, 1989-, et al.
(författare)
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Targeted Brain Delivery of Methotrexate by Glutathione PEGylated Liposomes : How can the Formulation Make a Difference?
- 2019
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Ingår i: European journal of pharmaceutics and biopharmaceutics. - : Elsevier BV. - 0939-6411 .- 1873-3441. ; 139, s. 197-204
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study was to quantitatively investigate how conjugation of GSH to different liposomal formulations influence the brain delivery of methotrexate (MTX) in rats. GSH-PEG liposomal MTX based on hydrogenated soy phosphatidylcholine (HSPC) or egg yolk phosphatidylcholine (EYPC) and their corresponding PEG control liposomes were prepared. The brain delivery of MTX after intravenously administering free MTX, four liposomal formulations or free MTX + empty GSH-PEG-HSPC liposomes was evaluated by performing microdialysis in brain interstitial fluid and blood. Compared to free MTX with a steady-state unbound brain-toplasma concentration ratio (K-p,K-uu) of 0.10, PEG-HSPC liposomes did not affect the brain uptake of MTX, while PEG-EYPC liposomes improved the uptake (K-p,(uu) 1.5, p < 0.05). Compared to PEG control formulations, GSHPEG-HSPC liposomes increased brain delivery of MTX by 4-fold (K-p,(uu) 0.82, p < 0.05), while GSH-coating on PEG-EYPC liposomes did not result in a further enhancement in uptake. The co-administration of empty GSHPEG-HSPC liposomes with free MTX did not influence the uptake of MTX into the brain. This work showed that the brain-targeting effect of GSH-PEG liposomal MTX is highly dependent on the liposomal formulation that is combined with GSH, providing insights on formulation optimization of this promising brain delivery platform.
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| 2879. |
- Hu, Yang, et al.
(författare)
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The Impact of Liposomal Formulations on the Release and Brain Delivery of Methotrexate : An In Vivo Microdialysis Study
- 2017
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Ingår i: Journal of Pharmaceutical Sciences. - : Elsevier BV. - 0022-3549 .- 1520-6017. ; 106:9, s. 2606-2613
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Tidskriftsartikel (refereegranskat)abstract
- The impact of liposomal formulations on the in vivo release and brain delivery of methotrexate (MTX) was quantitatively assessed in rats. Two PEGylated liposomal MTX formulations based on hydrogenated soy phosphatidylcholine (HSPC) or egg-yolk phosphatidylcholine (EYPC) were prepared. The drug release and uptake into the brain after intravenous administration of both formulations were compared with unformulated MTX by determining the released, unbound MTX in brain and plasma using microdialysis. Total MTX concentrations in plasma were determined using regular blood sampling. The administration of both high-and low-dose EYPC liposomes resulted in 10 times higher extent of MTX release in plasma compared to that obtained from HSPC liposomes (p < 0.05). MTX itself possessed limited brain uptake with steady-state unbound brain-to-plasma concentration ratio (K-p,K-uu) of 0.10 +/- 0.06. Encapsulation in HSPC liposomes did not affect MTX brain uptake (K-p,K-uu 0.11 +/- 0.05). In contrast, EYPC liposomes significantly improved MTX brain delivery with a 3-fold increase of Kp, uu (0.28 +/- 0.14 and 0.32 +/- 0.13 for high-and low-dose EYPC liposomal MTX, respectively, p < 0.05). These results provide unique quantitative evidence that liposomal formulations based on different phospholipids can result in very different brain delivery of MTX.
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| 2880. |
- Hua, Y., et al.
(författare)
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Molecular characteristics of eae-positive clinical Shiga toxin-producing Escherichia coli in Sweden
- 2020
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Ingår i: Emerging microbes & infections. - : Informa UK Limited. - 2222-1751. ; 9:1, s. 2562-2570
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Tidskriftsartikel (refereegranskat)abstract
- Shiga toxin (Stx)-producing Escherichia coli (STEC) can cause a wide range of symptoms from asymptomatic carriage, mild diarrhea to bloody diarrhea (BD) and hemolytic uremic syndrome (HUS). Intimin, encoded by the eae gene, also plays a critical role in STEC pathogenesis. Herein, we investigated the prevalence and genetic diversity of eae among clinical STEC isolates from patients with diarrhea, BD, HUS as well as from asymptomatic STEC-positive individuals in Sweden with whole-genome sequencing. We found that 173 out of 239 (72.4%) of clinical STEC strains were eae positive. Six eae subtypes (epsilon 1, gamma 1, beta 3, theta, zeta and rho) were identified eae and its subtype gamma 1 were significantly overrepresented in O157:H7 strains isolated from BD and HUS patients. epsilon 1 was associated with O121:H19 and O103:H2 strains, and beta 3 to O26:H11 strains. The combination of eae subtype gamma 1 and stx subtype (stx (2) or stx (1)+stx (2)) is more likely to cause severe disease, suggesting the possibility of using eae genotypes in risk assessment of STEC infection. In summary, this study demonstrated a high prevalence of eae in clinical STEC strains and considerable genetic diversity of eae in STEC strains in Sweden from 1994 through 2018, and revealed association between eae subtypes and disease severity.
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