| 61. |
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| 62. |
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| 63. |
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| 64. |
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| 65. |
- Blom, Thomas, et al.
(författare)
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Phenotypic characterization of KU812, a cell line identified as an immature human basophilic leukocyte
- 1992
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Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 22:8, s. 2025-2032
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Tidskriftsartikel (refereegranskat)abstract
- The knowledge about the differentiation of basophilic leukocytes is fragmentary. This report discusses a detailed phenotypic characterization of molecular markers for hematopoietic differentiation in a basophilic leukemia cell line, KU812. The expression of markers for lymphoid, erythroid, neutrophil, eosinophil, monocytic, megakaryocytic, mast cell and basophil differentiation was analyzed at the mRNA level by Northern blots in the KU812 cells, and for reference, in a panel of human cell lines representative of the different hematopoietic differentiation lineages. KU812 was found to express a number of mast cell and basophil-related proteins, i.e. mast cell tryptase, mast cell carboxypeptidase A, high-affinity immunoglobulin (IgE) receptor α and γ chains and the core protein for heparin and chondroitin sulphate synthesis. We found no expression of a number of monocyte/-macrophage or neutrophil leukocyte markers except for lysozyme. From earlier studies, it has been shown that lysozyme is not expressed in murine mucosal mast cell lines. This finding, together with the expression of the mast cell carboxypeptidase in KU812 might distinguish the phenotype of this cell line from that typical of mucosal mast cell lines in rodents. We found a low level of expression of the eosinophil and basophil marker, major basic protein, which might indicate a relationship between basophils and eosinophils. No expression is, however, detected with the eosinophil-specific markers, eosinophil cationic protein, eosinophil-derived neurotoxin or eosinophil peroxidase. We also report an extensive screening for inducers of basophilic differentiation of the KU812 cells. The most efficient protocol of induction included serum starvation which led to a dramatic increase in a number of markers specific for mast cells and basophils such as tryptase, carboxypeptidase A and the heparin core protein. Finally, diisopropylfluorophosphate analysis of total protein extracts from KU812 show four labeled protein bands with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating that this cell line expresses at least three previously undescribed serine proteases of which one or more could be a potential basophil-specific marker(s).
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| 66. |
- Blom, Thomas, 1961-, et al.
(författare)
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Pilgrimage or Sacred Tourism? : A Modern Phenomenon eith Historical Roots, with Examples from Fatima and Santiago de Compostela
- 2008
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Ingår i: Revista Turismo & Desenvolvimento. - Aveiro. - 1645-9261.
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Tidskriftsartikel (refereegranskat)abstract
- This article discusses the phenomenon of pilgrim tourism from a post-modern perspective characterized by an increasing secularization and illustrates, with the aid of a couple of examples - Santiago de Compostela in SpainspainSubject Category: Geographic Entitiessee more details and Fatima in PortugalportugalSubject Category: Geographic Entitiessee more details - the distinctive features of this type of tourism-related product and links it to a regional developmentregional developmentSubject Category: Miscellaneoussee more details perspective. It is increasingly obvious that the world is becoming more polarized with an orthodox conservative religious wind blowing across the world while an increasingly secular view of life is gaining ground reducing religiousness in daily life to an inferior position. The importance of the desire to visit religious sites to find fellowship and achieve mental satisfaction is gaining ground. Irrespective of the individual's purpose in making their journey to various sacred sites in the world, the tourism industry is growing in importance here and thus, in the future, ever greater demands will be placed on the regional and local actors who receive visitors at these sites. There are considerable opportunities for these actors to further develop sacred tourism as a post-modern tourism niche.
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| 67. |
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| 68. |
- Blom, Thomas, et al.
(författare)
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Profinite ∞-operads
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- We show that a profinite completion functor for (simplicial or topological) operads with good homotopical properties can be constructed as a left Quillen functor from an appropriate model category of ∞-operads to a certain model category of profinite ∞-operads. The construction is based on a notion of lean ∞-operad, and we characterize those ∞-operads weakly equivalent to lean ones in terms of homotopical finiteness properties. Several variants of the construction are also discussed, such as the cases of unital (or closed) ∞-operads and of ∞-categories.
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| 69. |
- Blom, Thomas, et al.
(författare)
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Profinite ∞-operads
- 2022
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Ingår i: Advances in Mathematics. - : Elsevier BV. - 0001-8708 .- 1090-2082. ; 408
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Tidskriftsartikel (refereegranskat)abstract
- We show that a profinite completion functor for (simplicial or topological) operads with good homotopical properties can be constructed as a left Quillen functor from an appropriate model category of ∞-operads to a certain model category of profinite ∞-operads. The construction is based on a notion of lean ∞-operad, and we characterize those ∞-operads weakly equivalent to lean ones in terms of homotopical finiteness properties. Several variants of the construction are also discussed, such as the cases of unital (or closed) ∞-operads and of ∞-categories.
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| 70. |
- Blom, Thomas
(författare)
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Regioners interaktion -Sverige och det
- 1990
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Ingår i: Geografisk utbildning och forskning. Gustafsson, G. (red). - Karlstad : Forskningsrapport 90:11, Högskolan i Karlstad.
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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