Tyck till om SwePub Sök
här!
Search: onr:"swepub:oai:DiVA.org:umu-114645" >
Reproducibility of ...
Reproducibility of telomere length assessment : an international collaborative study
-
Martin-Ruiz, Carmen M (author)
-
Baird, Duncan (author)
-
Roger, Laureline (author)
-
show more...
-
Boukamp, Petra (author)
-
Krunic, Damir (author)
-
Cawthon, Richard (author)
-
Dokter, Martin M (author)
-
van der Harst, Pim (author)
-
Bekaert, Sofie (author)
-
de Meyer, Tim (author)
-
- Roos, Göran (author)
- Umeå universitet,Patologi
-
- Svenson, Ulrika (author)
- Umeå universitet,Patologi
-
Codd, Veryan (author)
-
Samani, Nilesh J (author)
-
McGlynn, Liane (author)
-
Shiels, Paul G (author)
-
Pooley, Karen A (author)
-
Dunning, Alison M (author)
-
Cooper, Rachel (author)
-
Wong, Andrew (author)
-
Kingston, Andrew (author)
-
von Zglinicki, Thomas (author)
-
show less...
-
(creator_code:org_t)
- 2014-09-19
- 2015
- English.
-
In: International Journal of Epidemiology. - : Oxford University Press. - 0300-5771 .- 1464-3685. ; 44:5, s. 1673-1683
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://umu.diva-por... (primary) (Raw object)
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Background: Telomere length is a putative biomarker of ageing, morbidity and mortality. Its application is hampered by lack of widely applicable reference ranges and uncertainty regarding the present limits of measurement reproducibility within and between laboratories. Methods: We instigated an international collaborative study of telomere length assessment: 10 different laboratories, employing 3 different techniques [Southern blotting, single telomere length analysis (STELA) and real-time quantitative PCR (qPCR)] performed two rounds of fully blinded measurements on 10 human DNA samples per round to enable unbiased assessment of intra- and inter-batch variation between laboratories and techniques. Results: Absolute results from different laboratories differed widely and could thus not be compared directly, but rankings of relative telomere lengths were highly correlated (correlation coefficients of 0.63-0.99). Intra-technique correlations were similar for Southern blotting and qPCR and were stronger than inter-technique ones. However, inter-laboratory coefficients of variation (CVs) averaged about 10% for Southern blotting and STELA and more than 20% for qPCR. This difference was compensated for by a higher dynamic range for the qPCR method as shown by equal variance after z-scoring. Technical variation per laboratory, measured as median of intra- and inter-batch CVs, ranged from 1.4% to 9.5%, with differences between laboratories only marginally significant (P = 0.06). Gel-based and PCR-based techniques were not different in accuracy. Conclusions: Intra- and inter-laboratory technical variation severely limits the usefulness of data pooling and excludes sharing of reference ranges between laboratories. We propose to establish a common set of physical telomere length standards to improve comparability of telomere length estimates between laboratories.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Public Health, Global Health, Social Medicine and Epidemiology (hsv//eng)
Keyword
- Ageing
- telomeres
- variation
- biomarker
- human
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Martin-Ruiz, Car ...
-
Baird, Duncan
-
Roger, Laureline
-
Boukamp, Petra
-
Krunic, Damir
-
Cawthon, Richard
-
show more...
-
Dokter, Martin M
-
van der Harst, P ...
-
Bekaert, Sofie
-
de Meyer, Tim
-
Roos, Göran
-
Svenson, Ulrika
-
Codd, Veryan
-
Samani, Nilesh J
-
McGlynn, Liane
-
Shiels, Paul G
-
Pooley, Karen A
-
Dunning, Alison ...
-
Cooper, Rachel
-
Wong, Andrew
-
Kingston, Andrew
-
von Zglinicki, T ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Health Sciences
-
and Public Health Gl ...
- Articles in the publication
-
International Jo ...
- By the university
-
Umeå University