| 1. |
- Ahmad, Shabbir, et al.
(författare)
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Trimeric microsomal glutathione transferase 2 displays one third of the sites reactivity
- 2015
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Ingår i: Biochimica et Biophysica Acta - Proteins and Proteomics. - : Elsevier BV. - 1570-9639 .- 1878-1454. ; 1854:1010 Pt A, s. 1365-1371
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Tidskriftsartikel (refereegranskat)abstract
- Human microsomal glutathione transferase 2 (MGST2) is a trimeric integral membrane protein that belongs to the membrane-associated proteins in eicosanoid and glutathione metabolism (MAPEG) family. The mammalian MAPEG family consists of six members where four have been structurally determined. MGST2 activates glutathione to form a thiolate that is crucial for GSH peroxidase activity and GSH conjugation reactions with electrophilic substrates, such as 1-chloro-2,4-dinitrobenzene (CDNB). Several studies have shown that MGST2 is able to catalyze a GSH conjugation reaction with the epoxide LTA(4) forming the pro-inflammatory LTC4. Unlike its closest homologue leukotriene C-4 synthase (LTC4S), MGST2 appears to activate its substrate GSH using only one of the three potential active sites [Ahmad S, et al. (2013) Biochemistry. 52, 1755-1764]. In order to demonstrate and detail the mechanism of one-third of the sites reactivity of MGST2, we have determined the enzyme oligomeric state, by Blue native PAGE and Differential Scanning Calorimetry, as well as the stoichiometty of substrate and substrate analog inhibitor binding to MGST2, using equilibrium dialysis and Isothermal Titration Calorimetry, respectively. Global simulations were used to fit kinetic data to determine the catalytic mechanism of MGST2 with GSH and CDNB (1-chloro-2,4-dinitrobenzene) as substrates. The best fit was observed with 1/3 of the sites catalysis as compared with a simulation where all three sites were active. In contrast to LTC4S, MGST2 displays a 1/3 the sites reactivity, a mechanism shared with the more distant family member MGST1 and recently suggested also for microsomal prostaglandin E synthase-1.
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| 2. |
- Ahmad, Shakeel, et al.
(författare)
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Two-Tone PLL for on-Chip IP3 Test
- 2010
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Ingår i: Swedish System-on-Chip Conference.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 3. |
- Ahmad, T., et al.
(författare)
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Frequency and outcomes of undiagnosed diabetes mellitus in patients presenting with acute myocardial infarction
- 2020
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Ingår i: Medical Forum Monthly. - : Medical Forum Monthly. - 1029-385X. ; 31:12, s. 3-7
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To find out frequency and outcomes of undiagnosed diabetes mellitus in patients presenting with acute ST elevation myocardial infarction (STEMI). Study Design: Descriptive / Cross-Sectional Study Place and Duration of study: This study was conducted at the Cardiology Department, Lady Reading Hospital, Peshawar from November 2018 to May 2019. Materials and Methods: Patient of either gender having age ranging between 30-75 years old with acute STEMI who present within 12 hours of symptoms and with no past history of documented diabetes mellitus were included in the study. Venous blood samples for laboratory data, including random blood sugar, two fasting blood sugar and HBA1c using hitachi modular evo p800 machine was done. Results: A total of 158 patients having acute STEMI were studied. Males were 68.4% (n=108).The mean age was 59.65 ±10.80 years. Frequency of undiagnosed diabetes mellitus was 31.64 % (n = 50). In non-diabetics stress hyperglycemia was found in 51.85 % (n=56) patients. Among various types of STEMI, anterior STEMI was more common presentation 34.1 % (n=54. p= 0.85). Mean HBA1C was 6.19 ± 1.87%. Frequency of Ventricular tachycardia (VT) was 22.2 % in which undiagnosed diabetics were n=18 (p=0.004).Ventricular fibrillation was present in 13.3 % patients with undiagnosed diabetics were n=14 (p=0.001). Frequency of AF was 13.9% (n=22) with undiagnosed diabetics having AF in n=13 (p=0.003). SVT was present in 5.7% (n=9) patients with not significant difference between two groups (p=0.017). Among various mechanical complications VSR was present in 10 % (n=16) of patients (p=0.001), cardiogenic shock in 11.1 % (n=18) patients (p=0.004), acute LVF was present in 15.8 % patients (p=0.017). Conclusion: In our study we concluded that one third of patients having acute ST elevation myocardial infarction have undiagnosed diabetes mellitus (31.64 %, n = 50). The most common complication was ventricular tachycardia among electrical complication and LVF among mechanical complication.
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| 4. |
- Aad, G., et al.
(författare)
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- 2014
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Ingår i: Physical Review Letters. - 1079-7114 .- 0031-9007. ; 112:9
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Tidskriftsartikel (refereegranskat)
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| 5. |
- Chu, Thi My Chinh, et al.
(författare)
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Downlink outage analysis for cognitive cooperative radio networks employing non-orthogonal multiple access
- 2018
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Ingår i: 2018 IEEE 7th International Conference on Communications and Electronics, ICCE 2018. - : Institute of Electrical and Electronics Engineers Inc.. - 9781538636787 ; , s. 27-32
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Konferensbidrag (refereegranskat)abstract
- In this paper, we employ power-domain non-orthogonal multiple access (NOMA) to simultaneously transmit signals to both a primary user (PU) and a secondary user (SU) of a cognitive cooperative radio network (CCRN). Higher priority is given to the PU over the SU by keeping the power allocation coefficients at the base station (BS) and relay (R) above a certain threshold. In this way, similar as the interference power limit imposed by the PU in a conventional underlay CCRN, the power allocation coefficients at the BS and R of the CCRN can be controlled to maintain a given outage performance. Analytical expressions of the cumulative distribution function of the end-to-end signal-to-interference-plus-noise ratios at the PU and SU are derived and then used to assess the outage probabilities of both users. Numerical results are presented to study the impact of system parameters on outage performance of the CCRN with power-domain NOMA. In addition, it is illustrated that increased downlink performance can be obtained by combining power-domain NOMA with CCRNs. © 2018 IEEE.
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| 6. |
- Lalander, Philip, 1965-
(författare)
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Socialt arbete som situerad etik
- 2018. - 1st
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Ingår i: Manifest. - : Studentlitteratur AB. - 9789144125688 ; , s. 139-150
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Med utgångspunkt i forskning om heroinerfarna diskuterar Lalander relationernas betydelse för socialt arbete. Kärnan i det sociala arbetet är att skapa känslor av mänskligt värde, att människor känner sig respekterade.
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| 7. |
- Muranyi, Andreas
(författare)
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EGF-like modules in blood coagulation proteins : Ca²+ binding, module interactions, structure and dynamics as studied by NMR spectroscopy
- 2000
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Modules are independently folding protein domains defined on the gene level. The epidermal growth factor-like (EGF) modules are involved in protein-protein interactions and are found in numerous membrane proteins and extracellular proteins, including many proteins of the blood coagulation system. A subset of the EGF modules binds one Ca2+ with an affinity that is often influenced by the neighbouring module on the N-terminal side. The complex of factor VIIa (FVIIa) and tissue factor (TF) is important for the initiation of blood coagulation. Reports in the literature suggest that Ca2+ binding to the N-terminal EGF module (EGF 1) of FVIIa is essential for the complex to form. We determined the structure of the Ca2+-free EGF 1 using NMR spectroscopy. Comparison of this structure with that of the Ca2+-bound EGF 1 in the structure of the TF:FVIIa complex shows that only small conformational changes take place as a result of Ca2+ binding. These observations are consistent with the view that the Ca2+ binding to EGF 1 is crucial for a well-defined, relative orientation of the Gla and EGF 1 modules, which enables the formation of a high-affinity TF:FVIIa complex. Anticoagulant protein S has four EGF modules. The three C-terminal bind Ca2+ with high affinity. The fragment constituting the EGF 3-4 module pair from protein S (pS EGF 3-4) is the smallest fragment that retains high-affinity Ca2+ binding. The Kd for Ca2+ binding was determined to be 4.8 millimolar and 1.0 micromolar for EGF 3 and EGF 4, respectively. Thus the Ca2+ affinity of the N-terminal site was similar to that of the isolated EGF 3, while the affinity of EGF 4 in pS EGF 3-4 was approximately 9000-fold higher than that of the isolated EGF 4. The 1H, 15N, 13CA and 13CB resonances of the module pair were assigned using multidimensional heteronuclear NMR spectroscopy. The effect of Ca2+ binding on individual resonances was studied. Apart from extensive shift effects of resonances close to the Ca2+-binding site, we observed shift effects far from the expected location of the binding site in each of the modules. Extensive spectral heterogeneity revealed cis-trans isomerisation at a very slow rate (kex < 0.2 s-1) of the Lys 167-Pro 168 peptide bond or, possibly, trapping of the two conformers in the folding process. Both conformers have similar Ca2+ affinities and backbone dynamics. 15N spin relaxation data suggested that the module pair with one Ca2+ bound in EGF 4 has a well-defined relative orientation between EGF modules 3 and 4. In the absence of Ca2+, broadening of several resonances in EGF 4 suggests that chemical exchange is taking place. This is probably not consistent with a well-defined module interface. A comparison of residual dipolar couplings measured on a partly aligned pS EGF 3-4 sample with couplings calculated from the known structure of an EGF module pair from fibrillin-1 suggested that the two EGF modules of pS EGF 3-4 are oriented at an angle of approximately 90 º rather than being oriented in a rod-like arrangement (180 º) as in the fibrillin-1 EGF module pair.
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| 8. |
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| 9. |
- Atefyekta, Saba, et al.
(författare)
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Antimicrobial performance of mesoporous titania thin films : role of pore size, hydrophobicity, and antibiotic release.
- 2016
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Ingår i: International Journal of Nanomedicine. - 1176-9114 .- 1178-2013. ; 11, s. 977-90
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Tidskriftsartikel (refereegranskat)abstract
- Implant-associated infections are undesirable complications that might arise after implant surgery. If the infection is not prevented, it can lead to tremendous cost, trauma, and even life threatening conditions for the patient. Development of an implant coating loaded with antimicrobial substances would be an effective way to improve the success rate of implants. In this study, the in vitro efficacy of mesoporous titania thin films used as a novel antimicrobial release coating was evaluated. Mesoporous titania thin films with pore diameters of 4, 6, and 7 nm were synthesized using the evaporation-induced self-assembly method. The films were characterized and loaded with antimicrobial agents, including vancomycin, gentamicin, and daptomycin. Staphylococcus aureus and Pseudomonas aeruginosa were used to evaluate their effectiveness toward inhibiting bacterial colonization. Drug loading and delivery were studied using a quartz crystal microbalance with dissipation monitoring, which showed successful loading and release of the antibiotics from the surfaces. Results from counting bacterial colony-forming units showed reduced bacterial adhesion on the drug-loaded films. Interestingly, the presence of the pores alone had a desired effect on bacterial colonization, which can be attributed to the documented nanotopographical effect. In summary, this study provides significant promise for the use of mesoporous titania thin films for reducing implant infections.
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| 10. |
- Lalander, Philip, 1965-
(författare)
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Socialt arbete är politiskt
- 2021
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Ingår i: Alkohol & Narkotika. - Stockholm : Centralförbundet för alkohol- och narkotikaupplysning (CAN). - 0345-0732. ; :4
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- Värnandet av social rättvisa och strävan till social förändring kan idag låta radikalt, men egentligen är det värden som ligger i kärnan av det sociala arbetet, skriver Philip Lalander. I en tid av individuella förklaringar är ett kritiskt socialt arbete viktigare än någonsin.
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