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- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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- Ablikim, M., et al.
(författare)
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Amplitude analysis of the KSKS system produced in radiative J /psi decays
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 98:7
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Tidskriftsartikel (refereegranskat)abstract
- An amplitude analysis of the KSKS system produced in radiative J/psi decays is performed using the (1310.6 +/- 7.0) x 10(6) nip decays collected by the BESIII detector. Two approaches are presented. A mass-dependent analysis is performed by parametrizing the KSKS invariant mass spectrum as a sum of Breit-aligner line shapes. Additionally, a mass-independent analysis is performed to extract a piecewise function that describes the dynamics of the KSKS system while making minimal assumptions about the properties and number of poles in the amplitude. The dominant amplitudes in the mass-dependent analysis include the f(0)(1710), f(0)(2200), and f(2)'(1525). The mass-independent results, which are made available as input for further studies, are consistent with those of the mass-dependent analysis and are useful for a systematic study of hadronic interactions. The branching fraction of radiative J/psi decays to KSKS is measured to be (8.1 +/- 0.4) x 10(-4), where the uncertainty is systematic and the statistical uncertainty is negligible.
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| 8. |
- Ablikim, M., et al.
(författare)
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Measurement of the absolute branching fraction of D*(s0) (2317)(+/-) -> pi D-0(s)+/-
- 2018
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Ingår i: Physical Review D. - : American Physical Society. - 2470-0010 .- 2470-0029. ; 97:5
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Tidskriftsartikel (refereegranskat)abstract
- The process e(+)e(-) -> D*D-+(s)*(s0) (2317)(-) + c.c. is observed for the first time with the data sample of 567 pb(-1) collected with the BESIII detector operating at the BEPCII collider at a center-of-mass energy root s = 4.6 GeV. The statistical significance of the D*(s0) (2317)(+/-) signal is 5.8 sigma and the mass is measured to be (2318.3 +/- 1.2 +/- 1.2) MeV/c(2). The absolute branching fraction B(D*(s0) (2317)(+/-) -> pi D-0(s)+/-) is measured as 1.00(-0.14)(+0.00) (stat)(-0.14)(+0.00) (syst) for the first time. The uncertainties are statistical and systematic, respectively.
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- Ablikim, M., et al.
(författare)
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Measurement of the Absolute Branching Fraction of the Inclusive Decay Lambda(+)(c) -> Lambda plus X
- 2018
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Ingår i: Physical Review Letters. - : AMER PHYSICAL SOC. - 0031-9007 .- 1079-7114. ; 121:6
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Tidskriftsartikel (refereegranskat)abstract
- Based on an e(+)e(-) collision data sample corresponding to an integrated luminosity of 567 pb(-1) taken at the center-of-mass energy of root s = 4.6 GeV with the BESIII detector, we measure the absolute branching fraction of the inclusive decay Lambda(+)(c) -> Lambda + X to be B(Lambda(+)(c) -> Lambda + X) = (38.2(-2.2)(+2.8) +/- 0.9)% using the double-tag method, where X refers to any possible final state particles. In addition, we search for direct CP violation in the charge asymmetry of this inclusive decay for the first time, and obtain A(CP) [B(Lambda(+)(c) -> Lambda + X) - B((Lambda) over bar (-)(c) -> (Lambda) over bar + X)]/[B(Lambda(+)(c) -> Lambda + X) + B((Lambda) over bar (-)(c) -> (Lambda) over bar + X)] = (2.1(-6.6)(+7.0) +/- 1.6)%, a statistically limited result with no evidence of CP violation.
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- Ablikim, M., et al.
(författare)
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Observation of h(1)(1380) in the J/psi -> eta ' K(K)over-bar pi decay
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 98:7
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Tidskriftsartikel (refereegranskat)abstract
- Using 1.31 x 10(9) J/psi events collected by the BESIII detector at the BEPCII e(+)e(-) collider, we report the first observation of the h(1)(1380) in J/psi -> eta'h(1)(1380) with a significance of more than ten standard deviations. The mass and width of the possible axial-vector strangeonium candidate h(1)(1380) are measured to be M = (1423.2 +/- 2.1 +/- 7.3) MeV/c(2) and Gamma = (90.3 +/- 9.8 +/- 17.5) MeV. The product branching fractions, assuming no interference, are determined to be B(J/psi -> eta'h(1)(1380)) x B(h(1)(1380) -> K*(892)K-+(-)+c.c.) = (1.51 +/- 0.09 +/- 0.21) x 10(-4) in eta'K+K-pi(0) mode and B(J/psi -> eta'h(1)(1380)) x B(h(1)(1380) -> K*(892)(K) over bar +c.c.) =(2.16 +/- 0.12 +/- 0.29) x 10(-4) in eta'(KSK +/-)-K-0 pi(-/+) mode. The first uncertainties are statistical and the second are systematic. Isospin symmetry violation is observed in the decays h(1)(1380) K*(892)K-+(-) + c.c. and h(1)(1380) -> K*(892)(0)(K) over bar (0) + c.c.. Based on the measured h(1)(1380) mass, the mixing angle between the states h(1)(1170) and h(1)(1380) is determined to be (35.9 +/- 2.6)degrees, consistent with theoretical expectations.
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