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Mercury exposure as...
Mercury exposure as a model for deviation of cytokine responses in experimental Lyme arthritis : HgCl2 treatment decreases T helper cell type 1-like responses and arthritis severity but delays eradication of Borrelia burgdorferi in C3H/HeN mice
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- Ekerfelt, Christina, 1957- (author)
- Linköpings universitet,Klinisk immunologi,Hälsouniversitetet
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- Andersson, M. (author)
- Linköpings universitet,Klinisk immunologi,Hälsouniversitetet
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- Olausson, Anna, 1981- (author)
- Linköpings universitet,Klinisk immunologi,Hälsouniversitetet
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- Bergström, S. (author)
- Department of Microbiology, University of Umeå, Umeå, Sweden
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- Hultman, Per, 1957- (author)
- Linköpings universitet,Institutionen för molekylär och klinisk medicin,Hälsouniversitetet
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(creator_code:org_t)
- 2007-08-02
- 2007
- English.
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In: Clinical and Experimental Immunology. - : Oxford University Press (OUP). - 0009-9104 .- 1365-2249. ; 150:1, s. 189-197
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Lyme borreliosis is a complex infection, where some individuals develop so-called ‘chronic borreliosis’. The pathogenetic mechanisms are unknown, but the type of immune response is probably important for healing. A strong T helper cell type 1 (Th1)-like response has been suggested as crucial for eradication of Borrelia and for avoiding development of chronic disease. Many studies aimed at altering the Th1/Th2 balance in Lyme arthritis employed mice deficient in cytokine genes, but the outcome has not been clear-cut, due possibly to the high redundancy of cytokines. This study aimed at studying the importance of the Th1/Th2 balance in murine Borrelia arthritis by using the Th2-deviating effect of subtoxic doses of inorganic mercury. Ninety-eight C3H/HeN mice were divided into four groups: Borrelia-infected (Bb), Borrelia-infected exposed to HgCl2 (BbHg), controls exposed to HgCl2 alone and normal controls. Mice were killed on days 3, 16, 44 and 65 post-Borrelia inoculation. Arthritis severity was evaluated by histology, spirochaetal load determined by Borrelia culture, IgG2a- and IgE-levels analysed by enzyme-linked immunosorbemt assay (ELISA) and cytokine-secreting cells detected by enzyme-linked immunospot (ELISPOT). BbHg mice showed less severe histological arthritis, but delayed eradication of spirochaetes compared to Bb mice, associated with increased levels of IgE (Th2-induced) and decreased levels of IgG2a (Th1-induced), consistent with a Th2-deviation. Both the numbers of Th1 and Th2 cytokine-secreting cells were reduced in BbHg mice, possibly explained by the fact that numbers of cytokine-secreting cells do not correlate with cytokine concentration. In conclusion, this study supports the hypothesis that a Th1-like response is required for optimal eradication of Borrelia.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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