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Force-Induced Struc...
Force-Induced Structural Changes in Spider Silk Fibers Introduced by ATR-FTIR Spectroscopy
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- Jafari, Mohammad Javad (författare)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Bäcklund, Fredrik (författare)
- RISE,Polymera material och kompositer,Karolinska Institute, Sweden,Karolinska Inst, Sweden; RISE Res Inst Sweden, Sweden
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- Arndt, Tina (författare)
- Karolinska Institute, Sweden,Karolinska Inst, Sweden
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- Schmuck, Benjamin (författare)
- Karolinska Institute, Sweden; SLU Swedish University of Agricultural Sciences, Sweden,Karolinska Inst, Sweden; Swedish Univ Agr Sci, Sweden
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- Greco, Gabriele (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi (AFB),Department of Anatomy, Physiology and Biochemistry (AFB),Swedish Univ Agr Sci, Sweden
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- Rising, Anna (författare)
- Karolinska Institute, Sweden; SLU Swedish University of Agricultural Sciences, Sweden,Karolinska Inst, Sweden; Swedish Univ Agr Sci, Sweden
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- Barth, Andreas, 1960- (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm University, Sweden,Stockholm Univ, Sweden
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- Ederth, Thomas (författare)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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(creator_code:org_t)
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- American Chemical Society, 2023
- 2023
- Engelska.
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Ingår i: ACS applied polymer materials. - : American Chemical Society. - 2637-6105. ; 5:11, s. 9433-9444
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://res.slu.se/i...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Silk fibers have unique mechanical properties, and many studies of silk aim at understanding how these properties are related to secondary structure content, which often is determined by infrared spectroscopy. We report significant method-induced irreversible structural changes to both natural and synthetic spider silk fibers, derived from the widely used attenuated total reflection Fourier-transform infrared (ATR-FTIR) technique. By varying the force used to bring fibers into contact with the internal reflection elements of ATR-FTIR accessories, we observed correlated and largely irreversible changes in the secondary structure, with shape relaxation under pressure occurring within minutes. Fitting of spectral components shows that these changes agree with transformations from the alpha-helix to the beta-sheet secondary structure with possible contributions from other secondary structure elements. We further confirm the findings with IR microspectroscopy, where similar differences were seen between the pressed and unaffected regions of spider silk fibers. Our findings show that ATR-FTIR spectroscopy requires care in its use and in the interpretation of the results.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Textil-, gummi- och polymermaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Textile, Rubber and Polymeric Materials (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Annan materialteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Other Materials Engineering (hsv//eng)
Nyckelord
- silk fibers
- ATR-FTIR
- secondarystructure
- pressure effects
- spider silk
- NT2RepCT minispidroin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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