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Primary lymphocytes...
Primary lymphocytes as predictors for species differences in cytotoxic drug sensitivity
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- Hassan, Saadia B. (författare)
- Uppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics
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- Haglund, Caroline (författare)
- Uppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics
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- Åleskog, Anna (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Larsson, Rolf (författare)
- Uppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics
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- Lindhagen, Elin (författare)
- Uppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
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Ingår i: Toxicology in Vitro. - : Elsevier BV. - 0887-2333 .- 1879-3177. ; 21:6, s. 1174-1181
- Relaterad länk:
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Several in vitro methods have been suggested to predict drug-induced haematotoxicity and species differences; the most commonly used being the clonogenic CFU-GM assay. The aim of the current study was to evaluate whether primary lymphocytes from peripheral blood, assayed with a short-term non-clonogenic assay, could be used to detect species differences in drug sensitivity, and offer an alternative to the CFU-GM assay. The effect of 17 different cytotoxic drugs on lymphocytes from human, dog, rat and mouse was evaluated. A higher sensitivity of human than mouse lymphocytes was seen for topotecan and for 3 of 5 antimetabolites tested. Clear species specificity was also seen for the proteasome inhibitor bortezomib where rodent cells were 50–300 times less sensitive than human cells. Good agreement between our data and published CFU-GM data was observed, suggesting that primary lymphocytes may be a useful model for species difference screening in drug development.
Nyckelord
- Animals
- Antineoplastic Agents/*toxicity
- Cell Survival/drug effects
- Cells; Cultured
- Dogs
- Female
- Fluorometry
- Humans
- Leukocytes; Mononuclear/*drug effects
- Male
- Mice
- Mice; Inbred BALB C
- Rats
- Rats; Inbred Strains
- Species Specificity
- Toxicity Tests/methods
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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