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In situ identificat...
In situ identification of genes regulated specifically in fibroblasts of human basal cell carcinoma.
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- Micke, Patrick (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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Kappert, Kai (författare)
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Ohshima, Mitsuhiro (författare)
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Sundquist, Christina (författare)
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- Scheidl, Stefan Johannes, 1972 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Lindahl, Per, 1967 (författare)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberg Laboratory,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Institute of Medicine, Department of Molecular and Clinical Medicine
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- Heldin, Carl-Henrik (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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- Botling, Johan (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Pontén, Fredrik (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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Östman, Arne (författare)
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Botlinl, L (författare)
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
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Ingår i: The Journal of investigative dermatology. - : Elsevier BV. - 1523-1747 .- 0022-202X. ; 127:6, s. 1516-23
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Abstract
Ämnesord
Stäng
- Basal cell carcinoma (BCC) is characterized by slow growth, virtual absence of metastases, and strong stroma-dependency. Cancer-associated fibroblasts (CAFs) in the tumor stroma influence tumor growth, invasion, and metastasis. To comprehensively characterize CAFs of BCC in their in situ cancer environment, laser capture microdissection, linear gene amplification, microarray analysis, and quantitative real-time PCR (qRT-PCR) were combined. Pair-wise comparison of gene expression of microdissected CAFs and corresponding normal perifollicular fibroblasts identified 65 genes that were significantly upregulated in at least two of three different patients. Among the annotated genes, as many as 13 genes encoded secreted proteins, of which six were previously implicated as CAF-associated proteins in various tumor types. Four of the seven novel CAF genes--matrix Gla-protein, secreted frizzled-related protein 2, angiopoietin-related protein-2, and platelet-derived growth factor receptor-like protein--were selected for further analyses by qRT-PCR and were found to be frequently upregulated in CAFs of three independent BCC tissues. Analyses of CAFs from squamous cell cancer, prostate cancer, and colon cancer did not indicate that these genes were upregulated in these cancers. This study thus validates a novel approach for comprehensive characterization CAFs in their in situ environment of BCC. The results suggest a specific expression profile of CAFs in BCC possibly accounting for disease-specific pathological roles.
Nyckelord
- Carcinoma
- Basal Cell
- genetics
- pathology
- Fibroblasts
- physiology
- Gene Expression Profiling
- Gene Expression Regulation
- Neoplastic
- Humans
- Microdissection
- Oligonucleotide Array Sequence Analysis
- Reverse Transcriptase Polymerase Chain Reaction
- Skin Neoplasms
- genetics
- pathology
- Malignant tumor
- MEDICINE
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Micke, Patrick
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Kappert, Kai
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Ohshima, Mitsuhi ...
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Sundquist, Chris ...
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Scheidl, Stefan ...
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Lindahl, Per, 19 ...
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visa fler...
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Heldin, Carl-Hen ...
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Botling, Johan
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Pontén, Fredrik
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Östman, Arne
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Botlinl, L
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visa färre...
- Artiklar i publikationen
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The Journal of i ...
- Av lärosätet
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Göteborgs universitet
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Uppsala universitet
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Karolinska Institutet