Effects of inhibition of glycation and oxidative stress on the development of diabetic nephropathy in rats.

• We investigated whether aminoguanidine (AG), an inhibitor of advanced glycated end product formation, or probucol (PB), a free radical scavenger, could influence signs of glomerular and distal tubular function and morphological changes in kidneys of male Wistar rats after 6 months of streptozocin (STZ)-induced diabetes. Diabetic rats had a higher kidney weight/body weight ratio (P<.001), but neither AG nor PB influenced the increased ratio. Diabetes caused an increased urinary albumin excretion (P<.05), which was normalized by AG, but further exaggerated by PB (P<.001). Diabetes also caused an increase in the urinary excretion of Tamm–Horsfall protein (P<.001). Both AG and PB attenuated this increase (P<.05 for both). A few glomeruli displayed focal thickening of varying degrees. Silver staining disclosed the glomerulopathy to be intercapillary glomerulosclerosis. Rats on PB-enriched diet displayed less pronounced changes than untreated rats (P<.01), while AG had no effect. The results suggest that oxidative stress could be involved in the development of diabetic nephropathy.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)

Nyckelord

Free Radical Scavengers: pharmacology

Diabetic Nephropathies: physiopathology

Disease Progression

Experimental: physiopathology

Probucol: pharmacology

Rats

Animal

Diabetes Mellitus

Non-U.S. Gov't

Distal: physiopathology

Male

Oxidative Stress: physiology

Support

Wistar

Kidney Tubules

Distal: pathology

Distal: drug effects

Kidney Glomerulus: physiopathology

Kidney Glomerulus: pathology

Kidney Glomerulus: drug effects

Guanidines: pharmacology

Advanced: antagonists & inhibitors

Glycosylation End Products

Glycosylation

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)