| 1. |
- Aliakbari, Massume, et al.
(författare)
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Rubisco activase A (RcaA) is a central node in overlapping gene network of drought and salinity in Barley (Hordeum vulgare L.) and may contribute to combined stress tolerance
- 2021
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Ingår i: Plant physiology and biochemistry (Paris). - : Elsevier. - 0981-9428 .- 1873-2690. ; 161, s. 248-258
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Tidskriftsartikel (refereegranskat)abstract
- Co-occurrence of abiotic stresses, especially drought and salinity, is a natural phenomenon in field conditions and is worse for crop production than any single stress. Nowadays, rigorous methods of meta-analysis and systems biology have made it possible to perform cross-study comparisons of single stress experiments, which can uncover main overlapping mechanisms underlying tolerance to combined stress. In this study, a meta-analysis of RNA-Seq data was conducted to obtain the overlapping gene network of drought and salinity stresses in barley (Hordeum vulgare L.), which identified Rubisco activase A (RcaA) as a hub gene in the dual-stress response. Thereafter, a greenhouse experiment was carried out using two barley genotypes with different abiotic stress tolerance and evaluated several physiochemical properties as well as the expression profile and protein activity of RcaA. Finally, machine learning analysis was applied to uncover relationships among combined stress tolerance and evaluated properties. We identified 441 genes which were differentially expressed under both drought and salinity stress. Results revealed that the photosynthesis pathway and, in particular, the RcaA gene are major components of the dual-stress responsive transcriptome. Comparative physiochemical and molecular evaluations further confirmed that enhanced photosynthesis capability, mainly through regulation of RcaA expression and activity as well as accumulation of proline content, have a significant association with combined drought and salinity stress tolerance in barley. Overall, our results clarify the importance of RcaA in combined stress tolerance and may provide new insights for future investigations.
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| 2. |
- Almqvist, Gustaf, et al.
(författare)
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Report of the Benchmark Workshop on Baltic Cod Stocks (WKBALTCOD)
- 2015
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The ICES Benchmark Workshop on Baltic Cod Stocks (WKBALTCOD), chaired by External Chair Jean-Jacques Maguire, Canada and ICES Chair Marie Storr-Paulsen, Denmark, and attended by two invited external experts Verena Trenkel, France and Meaghan Bryan, USA met in Rostock, Germany, 2–6 March 2015 with 39 participants and six countries represented. The objective of WKBALTCOD was to evaluate the appropriateness of data and methods to determine stock status and investigate meth-ods appropriate to use in the single-stock assessment for the cod stock in SD 22–24 and cod in SD 25–32 in the Baltic. Participants in the workshop were a large group with diverse backgrounds representing the industry, fisheries, NGOs, managers and scientists.The single-stock analytic assessment of the eastern Baltic stock was not accepted by the assessment working group (WGBFAS) in 2014 due to severe problems with the input data. The advice for the eastern Baltic cod was, therefore, based on the ICES approach for data-limited stocks. As an outcome ICES decided to establish a bench-mark for both cod stocks and to scope an integrated assessment for the Baltic cod stocks. The first meeting (WKSIBCA) was therefore meant to introduce the interces-sional work conducted since the assessment working group in April 2014, and to reach some conclusions on how to proceed both in the short term (Benchmark in March 2015) and longer term (2–3 years) and was seen as a data compilation work-shop, there is produced a separate report from this workshop. The WKBALTCOD was the 2nd meeting in the benchmark process and was intended to come up with a final stock assessment method, stock annex and input data for both stocks. As it was not possible to reach conclusive decision on the final model to be used for the east Baltic cod stock during the benchmark meeting and as more work on the preferable models was needed, it was decided by the ACOM leadership to prolong the bench-mark process until the assessment working group meeting in April 2015. This deci-sion has led to a relatively long process partly mixed with the assessment working group WGBFAS.It became clear during the benchmark process that although large effort has been put into explaining the underlying processes leading to the changes in the Baltic ecosys-tem, there is still some lack of understanding of the present situation in the eastern Baltic cod stock. Therefore, it was not possible to reach firm conclusions on the final model to be used and therefore not possible to set reference points. It was decided to continue to explore the most promising models and to continue to improve the input data until the assessment working group started in April.The main challenges still to be solved for the Eastern Baltic cod stock is the quantifi-cation of increased natural mortality and decrease in growth. Through several presentations during the workshop (both WKSIBCA and WKBALTCOD) it became clear that natural mortality very likely has increased in later years, due to decreased condition and increased parasite infection. A decrease in growth also seems plausible duo to a decrease in condition and/or selectivity-induced mortality of the largest in-dividuals. However, as none of these parameters are easily estimated, especially with the severe ageing problems, different model assumptions made the output very shaky.For the western Baltic cod, stock identification issues were examined in area SD 24, the intermediate area: based on otolith characteristics and genetics. Due to the results showing a large proportion of east cod in this area, it was decided to split the catch2 | ICES WKBALTCOD REPORT 2015and survey from SD 24 into either the western or eastern Baltic cod stock. It was pos-sible to derive proportions of eastern and western cod in SD 24 back to the mid-1990s.For the western Baltic cod stock a modelled survey indices was included in the as-sessment covering the western part of SD 24 and Area 22+23 and based on a smoothed ALK.Both cod stocks have in the past used commercial tuning fleet to have a better cov-ered of older age groups. It was decided to abound this time-series duo quality issues such as a limited coverage and problems with technical creeping.WKBALTCOD was not able to explore and define reference points for the Western Baltic cod stock during the meeting due to time constraints, but these were calculated and decided by correspondence after the meeting. The recent protocols on estimation procedures developed by WKMSYREF3 for stocks with a full analytical assessment and for data-limited stocks served as objective guidelines to obtain reference point estimates.
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| 3. |
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| 4. |
- Aps, R., et al.
(författare)
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Central Baltic Sea herring : effect of environmental trends and fishery management
- 2011
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Ingår i: Ecosystems and Sustainable Development VIII. - Southampton : BioMed Central Ltd.. - 9781845645106 - 9781845645113 ; , s. 69-80
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Konferensbidrag (refereegranskat)abstract
- Uncertainty is an endemic condition of the Baltic Sea herring (Clupea harengus membras, L) fishery management. It is a condition exacerbated by the fishing fleet overcapacity and consequent exploitation of the herring stock at a level believed to be unsustainable. Some sources of uncertainty are mainly related to biology and fishing technique: the unsolved problem of herring assessment and management units, the recruitment–environment relationship and the reduction in mean weights-at-age, uncertain ageing of fish, the problem of unaccounted fishing mortality caused by the fish selection through the trawl net. Fishing fleet overcapacity is believed to be behind of the regulatory overfishing when setting the Total Allowable Catches (TACs) higher than the scientific advice (decision overfishing) and tolerating the extensive underreporting of catches (implementation overfishing). Two scenarios for the Central Baltic Sea herring fishery management options are constructed and the Bayesian networks are used to represent and update uncertainties encountered in the process of the management related situation assessment. First scenario represents the current status of the fishery management resulting in fishing mortality (F) higher than FMSY – the fishing mortality that corresponds to the Maximum Sustainable Yield (MSY). The second scenario demonstrates the assumed potential impact of economic incentives (e.g.zoning, individual transferable quotas (ITQs), territorial use rights etc.) on the reduction of excessive fishing capacity and bringing actual fishing mortality closer to FMSY.
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| 5. |
- Arvidsson, Daniel, 1974, et al.
(författare)
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Energy cost of physical activities in children : Validation of SenseWear Armband
- 2007
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Ingår i: Medicine & Science in Sports & Exercise. - : American College of Sports Medicine. - 0195-9131 .- 1530-0315. ; 39:11, s. 2076-2084
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Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: To examine the validity of SenseWear Pro2 Armband in assessing energy cost of physical activities in children, and to contribute with values of energy costs in an overview of physical activities in children. METHODS: Energy cost was assessed by SenseWear Pro2 Armband in 20 healthy children, 11-13 yr, while lying down resting, sitting playing games on mobile phone, stepping up and down on a step board, bicycling on a stationary bike, jumping on a trampoline, playing basketball, and walking/running on a treadmill at the speeds 2, 3, 4, 5, 6, 7, 8, and 10 km x h(-1). During these activities, energy cost was also assessed from VO2 and VCO2 measured by Oxycon Mobile portable metabolic system, which was used as criterion method. RESULTS: The difference in energy cost between SenseWear Pro2 Armband and Oxycon Mobile was -0.7 (0.5) (P < 0.001) for resting, -2.0 (0.9) (P < 0.001) for playing games on mobile phone, -6.6 (2.3) (P < 0.001) for stepping on the step board, -12.0 (3.7) (P < 0.001) for bicycling, -2.7 (11.9) (P = 0.34) for jumping on the trampoline, and -14.8 (6.4) kJ x min(-1) (P < 0.001) for playing basketball. The difference in energy cost between SenseWear Pro2 Armband and Oxycon Mobile for increasing treadmill speed was 1.3 (3.1) (P = 0.048), 0.1 (2.9) (P = 0.82), -1.2 (2.6) (P = 0.049), -1.6 (3.2) (P = 0.044), -3.1 (3.7) (P = 0.0013), -4.9 (3.7) (P < 0.001), -5.3 (3.7) (P < 0.001), and -11.1 (3.5) kJ x min(-1) (P < 0.001). CONCLUSIONS: SenseWear Pro2 Armband underestimated energy cost of most activities in this study, an underestimation that increased with increased physical activity intensity. A table of energy costs (MET values) of physical activities in children measured by indirect calorimetry is presented as an initiation of the creation of a compendium of physical activities in children
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| 6. |
- Backe, Stefan
(författare)
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The Effect of an Increased Sugar Intake in the Diet of People with Type 2 Diabetes
- 2024
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Ingår i: Journal of Clinical Epidemiology and Public Health. ; 2:3, s. 01-01
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Tidskriftsartikel (refereegranskat)abstract
- [The article introduces a coming project:]The idea is to carry out a cohort study, preferable for a year, where one group is allowed to eat and exercise as usual while the other group changes their diet towards a lower sugar diet.
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| 7. |
- Backe, Stefan
(författare)
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Type 2 Diabetes—Hard to Select a Healthy Choice
- 2024
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Ingår i: Health. - : Scientific Research Publishing. - 1949-4998 .- 1949-5005. ; 16:01, s. 1-8
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: The use of foods containing high levels of sugar is increasing all the time. This is a risk factor for increased incidence of type 2 diabetes. There are few studies that have investigated the availability of low-sugar müsli products in grocery stores. Purpose: The study aims to identify which types of müsli contain high respectively low levels of sugar, and which brands are involved. Methods: The material consists of both qualitative interviews and observations from five grocery stores: City Gross, Hemköp, Ica Maxi, Stora Coop and Willy’s in Helsingborg, Sweden. The qualitative interviews had a semi-structured character and were recorded. The interviews took approx. 20 minutes and a textual analysis was conducted of the results. Data from observation was analyzed based on brand, nutritional composition and flavors, and also, where low sugar products were placed on store shelves. Results: The grocery stores provided together brands from AXA, Coop, Finax, Frebaco, Garant, ICA, Risenta, Saltå Kvarn och Urtekram, in total 24 müsli products. Of these products, 19 were high in sugar. The observation reveals that müsli products with high sugar content (17 - 29 g per 100 g müsli) are more prominently displayed than those with low sugar content. From the interviews with the store managers, it became clear that it would be valuable to highlight healthy müsli products on the shelves. However, central bureaucracy puts obstacles to such measures. Discussion: The study emphasizes the need for increased visibility of low-sugar products and proposes solutions such as negotiating with responsible person at the head office in Stockholm. Several reviews have shown that if the grocery store raises the prices of unhealthy food, the consumer is willing to purchase healthier müsli and other products. Conclusion: This study shows the need for grocery stores to upgrade healthy müsli products along with advertising to be able to influence customer’s shopping habits. Also, further research is needed how type 2 diabetes is affected by high intakes of food products with high sugar content.
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| 8. |
- Backström, Tobias, et al.
(författare)
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CRF and urotensin I effects on aggression and anxiety-like behavior in rainbow trout
- 2011
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Ingår i: Journal of Experimental Biology. - : The Company of Biologists. - 0022-0949 .- 1477-9145. ; 214:6, s. 907-914
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Tidskriftsartikel (refereegranskat)abstract
- Corticotropin-releasing factor (CRF) is central in the stress response but also modulates several behaviors including anxiety-related behaviors and aggression. In this study, juvenile rainbow trout (Oncorhynchus mykiss) were tested for competitive ability, determined during dyadic fights for dominance, after intracerebroventricular (i.c.v.) administration of CRF, urotensin I (UI), the non-specific CRF antagonist alpha-helical RF9-41 (ahCRF) or the CRF receptor subtype 1-specific antagonist antalarmin, when paired with a mass-matched con-specific injected with saline. In addition, isolated fish received the same substances. Plasma cortisol and brain monoamines were monitored in all fish. Most fish receiving CRF showed a conspicuous behavior consisting of flaring the opercula, opening the mouth and violent shaking of the head from side to side. When this occurred, the fish immediately forfeited the fight. Similar behavior was observed in most fish receiving UI but no effect on outcome of dyadic fights was noted. This behavior seems similar to non-ambulatory motor activity seen in rats and could be anxiety related. Furthermore, fish receiving CRF at a dose of 1000. ng became subordinate, whereas all other treatments had no effects on the outcome of dyadic fights. In addition, isolated fish receiving ahCRF had lower brain stem concentrations of 5-hydroxyindoleacetic acid, serotonin, 3,4-dihydroxyphenylacetic acid and dopamine. In conclusion, CRF seems to attenuate competitive ability, and both CRF and UI seem to induce anxiety-like behavior.
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| 9. |
- Beetz, Andrea, et al.
(författare)
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The Effect of a Real Dog, Toy Dog and Friendly Person on Insecurely Attached Children During a Stressful Task : An Exploratory Study
- 2011
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Ingår i: Anthrozoos. - : Taylor & Francis. - 0892-7936 .- 1753-0377. ; 24:4, s. 349-368
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Tidskriftsartikel (refereegranskat)abstract
- The regulation of stress by an attachment figure is a key feature of attachment relationships. Previous research suggests that in some cases animal companionship may be regarded as an attachment relationship. This may be particularly important for persons with an insecure or disorganized attachment pattern who may find it more difficult than securely attached individuals to accept social support from humans. In our study, we investigated whether 31 boys (aged 7-12 years) with insecure/disorganized attachment would profit more from the presence of a dog (n = 11) than of a friendly human (n = 11) or a toy dog (n = 9) as support during a socially stressful situation (Trier Social Stress Test for Children, TSST-C). Stress levels were assessed via salivary cortisol recorded five times before, during, and after the TSST-C. The behavior of the children was coded from video recordings. Self-reported stress levels did not significantly differ between the groups before and after the TSST-C. Salivary cortisol, however, was significantly lower in the real dog condition than in the other two conditions (Kruskal-Wallis H test on area under the curve increase (AUCi): chi(2) = 15.17, df = 2, p = 0.001). Also, the more the children stroked the dog, the less pronounced was their stress reaction (r(s) = -0.818, p = 0.002). Our data suggest an important role of physical contact in the stress reducing effect. We conclude that the children investigated profited more from interacting with a friendly dog than with either a human or a toy dog in a stressful situation. We discuss the relevance of our findings for animal-assisted interventions.
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| 10. |
- Bergenius, Mikaela, et al.
(författare)
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Report of the Baltic Fisheries Assessment Working Group (WGBFAS) : 14-21 April 2015, ICES HQ, Copenhagen, Denmark
- 2015
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The ICES Baltic Fisheries Assessment Working Group (WGBFAS) met 14-21 April 2015 (Chair: Mare Storr-Paulsen, Denmark), with 28 participants and 9 countries represented. The objective of WGBFAS was to assess the status of the following stocks:1 ) Sole in Division IIIa, SDs 20-222 ) Cod in Kattegat, Cod in SD 22-24, Cod in SD 25-323 ) Herring in SD 25-27, 28.2, 29 and 32, Herring in SD 28.1 (Gulf of Riga), Herring in SD 30, Herring SD 31.4 ) Sprat in SD 22-325 ) Plaice 21-23, Plaice 24256 ) Flounder 22-23; 24-25; 26+28 and 27+29-32, Brill 2232, Dab 2232, and Turbot 2232 (survey trends)WGBFAS also identified the data needed, for next year’s data call with some suggestions for improvements in the data call as well as in InterCatch. The report contains an introduction with the summary of other WGs relevant for the WGBFAS, country specific fishery description, the methods used, and ecosystem considerations. The results of the analytical stock assessment or survey trends for the species listed above are then presented with all the stocks with the same species in the same sections. The report ends with references, list of Working Documents, recommendations and Stock Annexes. In first quarter 2015 the Baltic cod stocks and the plaice stocks were benchmarked. As a result the Baltic cod stocks now have to apply a splitting key in SD 24 were both stocks are present. This has changed the assessment from being an area based assessment to now being a stock based assessments and has implications for the advice. The principle analytical models used for the stock assessments were XSA and SAM. For most flatfishes, CPUE trends from bottom trawl surveys were presented (except plaice 2425 and her31 using relative SSB from SAM and XSA, respectively). Ecosystem changes have been analytically considered in the following stock assessments: Herring in SD 25-27, 28.2, 29 and 32, and Sprat in SD 22-32, in form of cod predation mortality. Last year a very large retrospective pattern in the Eastern Baltic cod stock caused that the WG rejected the analytic assessment. Several uncertainties in the data lead to this conclusion i.a age reading problems with large inconsistency between and within nations as well as a change in growth and natural mortality. However, even though a data compilation workshop and a benchmark have been conducted in the intermediate time it was not possible to solve the main issue on growth. The lack of knowledge on growth caused to that even the length based data required in the data call was very uncertain for the models and in the end the WG was not able to produce a better model than was presented last year which is based on survey trends. The Her-30 (Herring in the Botnian Sea) was by the working group down scaled from a category 1 stock to a category 3 stock due to the commercial tuning fleet used in the assessment having very uncertain estimates in the last couples of years. However, during the Baltic ADG an alternative assessment was suggested were the stock is still considered a category 1 stock but the last 8 years of the commercial tuning fleet was terminated. This assessment was conducted after the working group but has been included in the report.
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