| 1. |
- Srivastava, Vaibhav, et al.
(författare)
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OnPLS integration of transcriptomic, proteomic and metabolomic data shows multi-level oxidative stress responses in the cambium of transgenic hipI- superoxide dismutase Populus plants
- 2013
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Ingår i: BMC Genomics. - : BioMed Central. - 1471-2164. ; 14
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Reactive oxygen species (ROS) are involved in the regulation of diverse physiological processes in plants, including various biotic and abiotic stress responses. Thus, oxidative stress tolerance mechanisms in plants are complex, and diverse responses at multiple levels need to be characterized in order to understand them. Here we present system responses to oxidative stress in Populus by integrating data from analyses of the cambial region of wild-type controls and plants expressing high-isoelectric-point superoxide dismutase (hipI-SOD) transcripts in antisense orientation showing a higher production of superoxide. The cambium, a thin cell layer, generates cells that differentiate to form either phloem or xylem and is hypothesized to be a major reason for phenotypic perturbations in the transgenic plants. Data from multiple platforms including transcriptomics (microarray analysis), proteomics (UPLC/QTOF-MS), and metabolomics (GC-TOF/MS, UPLC/MS, and UHPLC-LTQ/MS) were integrated using the most recent development of orthogonal projections to latent structures called OnPLS. OnPLS is a symmetrical multi-block method that does not depend on the order of analysis when more than two blocks are analysed. Significantly affected genes, proteins and metabolites were then visualized in painted pathway diagrams.RESULTS: The main categories that appear to be significantly influenced in the transgenic plants were pathways related to redox regulation, carbon metabolism and protein degradation, e.g. the glycolysis and pentose phosphate pathways (PPP). The results provide system-level information on ROS metabolism and responses to oxidative stress, and indicate that some initial responses to oxidative stress may share common pathways.CONCLUSION: The proposed data evaluation strategy shows an efficient way of compiling complex, multi-platform datasets to obtain significant biological information.
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| 2. |
- Lindahl, Olof A, et al.
(författare)
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Biomedical engineering research improves the health care industry
- 2014
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Ingår i: XIII Mediterranean Conference on Medical and Biological Engineering and Computing 2013. - Cham : Springer. - 9783319008455 - 9783319008462 ; , s. 1124-1126
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Konferensbidrag (refereegranskat)abstract
- The health care industry is dependent on new innovations for its survival and expansion. Health care innovations are also important for improving patient care. Through activities at the centre for biomedical engineering and physics (CMTF) we have generated growth both in academia at the universities and in the industry in northern Sweden. Fruitful cooperation was generated between 26 research projects and about 15 established companies in the field of biomedical engineering. The established researcher-owned company for business development of the research results from the CMTF, CMTF Business Development Co Ltd, has so far launched three spin-off companies and has 10 new business leads to develop. The activities have also increased the interest for commercialization and entrepreneurship among the scientists in the centre. So far a total of nine spin-off companies have resulted from the CMTF-research since the year 2000 that has improved the health care market in northern Sweden. © Springer International Publishing Switzerland 2014.
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| 3. |
- Metreveli, Giorgi, et al.
(författare)
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A Size-Exclusion Nanocellulose Filter Paper for Virus Removal
- 2014
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Ingår i: Advanced Healthcare Materials. - : Wiley. - 2192-2640 .- 2192-2659. ; 10:3, s. 1546-1550
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Tidskriftsartikel (refereegranskat)abstract
- This is the first time a 100% natural, unmodified nanofibrous polymer-based membrane is demonstrated capable of removing viruses solely based on the size-exclusion principle, with log10 reduction value (LRV) ≥ 6.3 as limited by the assay lower detection limit and the feed virus titre, thereby matching the performance of industrial synthetic polymer virus removal filters.
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| 4. |
- Böhler, Christian, et al.
(författare)
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Multilayer Arrays for Neurotechnology Applications (MANTA): Chronically Stable Thin-Film Intracortical Implants
- 2023
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Ingår i: Advanced Science. - : John Wiley & Sons. - 2198-3844. ; 10:14
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Tidskriftsartikel (refereegranskat)abstract
- Flexible implantable neurointerfaces show great promise in addressing one of the major challenges of implantable neurotechnology, namely the loss of signal connected to unfavorable probe tissue interaction. The authors here show how multilayer polyimide probes allow high-density intracortical recordings to be combined with a reliable long-term stable tissue interface, thereby progressing toward chronic stability of implantable neurotechnology. The probes could record 10–60 single units over 5 months with a consistent peak-to-peak voltage at dimensions that ensure robust handling and insulation longevity. Probes that remain in intimate contact with the signaling tissue over months to years are a game changer for neuroscience and, importantly, open up for broader clinical translation of systems relying on neurotechnology to interface the human brain.
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| 5. |
- Shaner, Sebastian, et al.
(författare)
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Bioelectronic microfluidic wound healing: a platform for investigating direct current stimulation of injured cell collectives
- 2023
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Ingår i: Lab on a Chip. - : Royal Society of Chemistry. - 1473-0197 .- 1473-0189. ; 23:6, s. 1531-1546
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Tidskriftsartikel (refereegranskat)abstract
- Upon cutaneous injury, the human body naturally forms an electric field (EF) that acts as a guidance cue for relevant cellular and tissue repair and reorganization. However, the direct current (DC) flow imparted by this EF can be impacted by a variety of diseases. This work delves into the impact of DC stimulation on both healthy and diabetic in vitro wound healing models of human keratinocytes, the most prevalent cell type of the skin. The culmination of non-metal electrode materials and prudent microfluidic design allowed us to create a compact bioelectronic platform to study the effects of different sustained (12 hours galvanostatic DC) EF configurations on wound closure dynamics. Specifically, we compared if electrotactically closing a wound's gap from one wound edge (i.e., uni-directional EF) is as effective as compared to alternatingly polarizing both the wound's edges (i.e., pseudo-converging EF) as both of these spatial stimulation strategies are fundamental to the eventual translational electrode design and strategy. We found that uni-directional electric guidance cues were superior in group keratinocyte healing dynamics by enhancing the wound closure rate nearly three-fold for both healthy and diabetic-like keratinocyte collectives, compared to their non-stimulated respective controls. The motility-inhibited and diabetic-like keratinocytes regained wound closure rates with uni-directional electrical stimulation (increase from 1.0 to 2.8% h−1) comparable to their healthy non-stimulated keratinocyte counterparts (3.5% h−1). Our results bring hope that electrical stimulation delivered in a controlled manner can be a viable pathway to accelerate wound repair, and also by providing a baseline for other researchers trying to find an optimal electrode blueprint for in vivo DC stimulation.
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| 6. |
- Olsson, Rickard, 1959-, et al.
(författare)
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Production of osseointegrating (bone bonding) surfaces on titanium screws by laser melt disruption
- 2018
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Ingår i: Journal of Laser Applications. - Melville, NY : Laser Institute of America. - 1042-346X .- 1938-1387. ; 30:4
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Tidskriftsartikel (refereegranskat)abstract
- Several techniques can be used to modify implant surfaces in order to accelerate bone growth around titanium implants. One method is to generate a surface structure which stimulates bone growth and remodeling. This paper describes and explains a nonablative method for producing osseointegrating (structural and functional bone bonding) surfaces on titanium implants using laser processing. The focus is especially on surface texturing of dental implant screws, where the ability of a Nd:YAG laser to generate "splashy" surfaces covered in resolidified microscale droplets coated with nanoscale surface oxides is assessed. The surfaces produced were analyzed by a scanning electron microscope and energy dispersive x-ray spectroscopy. It is concluded that laser processing using Q-pulsed Nd:YAG lasers can generate surfaces which match the demands set by clinical experience. One important characteristic of the surfaces discussed here is that they involve overhanging features which are suitable for trapping red blood cells and which cannot be created by mechanical or chemical roughening techniques. © 2018 Laser Institute of America.
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| 7. |
- Vuddanda, Parameswara Rao, et al.
(författare)
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Electrospun nanofiber Mats for ultrafast release of ondansetron
- 2016
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Ingår i: Reactive & functional polymers. - : Elsevier BV. - 1381-5148 .- 1873-166X. ; 99, s. 65-72
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Tidskriftsartikel (refereegranskat)abstract
- Nanofiber mats or films are promising platforms that can offer unique opportunities in oromucosoal drug delivery. However, the conventional film forming technologies are unable to produce mats with unique internal microstructure and properties. Thus, the present study was aimed to develop electrospun nanofiber mats of a model drug -ondansetron hydrochloride (OND) for ultrafast drug release. Polyvinyl alcohol (PVA), a water soluble synthetic polymer was used in the preparation of nanofiber mats and casting film. The OND nanofiber mats and conventional films were prepared by electrospinning and casting methods, respectively. Different electrospinning process variables (feed rate, electric voltage and tip to collector distance) were investigated. Nanofiber mats and casted films were characterized using Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Differential scanning calorimetry (DSC), Powder X-ray diffraction (PXRD), and Attenuated total reflection – Fourier transform infrared spectroscopy (ATR-FTIR). The folding endurance, drug content, wetting behaviour and disintegration properties and in-vitro drug release studies were also performed.The SEM and AFM had revealed that the nanofiber mats were formed with smooth uniform texture. Solid state studies indicated that the OND was in amorphous state and uniformly dispersed in PVA mats and a film. The electrospun nanofiber mat and casted film of OND showed sufficient mechanical properties. Wet sponge method suggested that OND nanofiber mats were simultaneously wetted and disintegrated within 10 s, which is ultrafast compared to casted films. The total amount of OND was released in 90 s (1.5 min) and 1800 s (30 min) from OND-PVA electrospun nanofiber mats and casted film, respectively. OND nanofiber mats can be promising alternatives to existing solid dosage forms for ultrafast release of drugs.
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| 8. |
- Alrifaiy, Ahmed, et al.
(författare)
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How to integrate a micropipette into a closed microfluidic system : absorption spectra of an optically trapped erythrocyte
- 2011
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Ingår i: Biomedical Optics Express. - 2156-7085. ; 2:8, s. 2299-2306
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Tidskriftsartikel (refereegranskat)abstract
- We present a new concept of integrating a micropipette within a closed microfluidic system equipped with optical tweezers and a UV-Vis spectrometer. A single red blood cell (RBC) was optically trapped and steered in three dimensions towards a micropipette that was integrated in the microfluidic system. Different oxygenation states of the RBC, triggered by altering the oxygen content in the microchannels through a pump system, were optically monitored by a UV-Vis spectrometer. The built setup is aimed to act as a multifunctional system where the biochemical content and the electrophysiological reaction of a single cell can be monitored simultaneously. The system can be used for other applications like single cell sorting, in vitro fertilization or electrophysiological experiments with precise environmental control of the gas-, and chemical content.
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| 9. |
- Baş, Yağmur, et al.
(författare)
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Preparation and Characterization of Softwood and Hardwood Nanofibril Hydrogels: Toward Wound Dressing Applications
- 2023
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Ingår i: Biomacromolecules. - : American Chemical Society (ACS). - 1525-7797 .- 1526-4602. ; 24:12, s. 5605-5619
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Tidskriftsartikel (refereegranskat)abstract
- Hydrogels of cellulose nanofibrils (CNFs) are promising wound dressing candidates due to their biocompatibility, high water absorption, and transparency. Herein, two different commercially available wood species, softwood and hardwood, were subjected to TEMPO-mediated oxidation to proceed with delignification and oxidation in a one-pot process, and thereafter, nanofibrils were isolated using a high-pressure microfluidizer. Furthermore, transparent nanofibril hydrogel networks were prepared by vacuum filtration. Nanofibril properties and network performance correlated with oxidation were investigated and compared with commercially available TEMPO-oxidized pulp nanofibrils and their networks. Softwood nanofibril hydrogel networks exhibited the best mechanical properties, and in vitro toxicological risk assessment showed no detrimental effect for any of the studied hydrogels on human fibroblast or keratinocyte cells. This study demonstrates a straightforward processing route for direct oxidation of different wood species to obtain nanofibril hydrogels for potential use as wound dressings, with softwood having the most potential.
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| 10. |
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