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- Wiszmeg, Andréa, et al.
(författare)
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Transforming trash to treasure Cultural ambiguity in foetal cell research
- 2021
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Ingår i: Philosophy Ethics and Humanities in Medicine. - : Springer Science and Business Media LLC. - 1747-5341. ; 16:1
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Tidskriftsartikel (refereegranskat)abstract
- Background Rich in different kind of potent cells, embryos are used in modern regenerative medicine and research. Neurobiologists today are pushing the boundaries for what can be done with embryos existing in the transitory margins of medicine. Therefore, there is a growing need to develop conceptual frameworks for interpreting the transformative cultural, biological and technical processes involving these aborted, donated and marginal embryos. This article is a contribution to this development of frameworks. Methods This article examines different emotional, cognitive and discursive strategies used by neurobiologists in a foetal cell transplantation trial in Parkinson's disease research, using cells harvested from aborted embryos. Two interviews were analysed in the light of former observations in the processing laboratories, using the anthropologist Mary Douglas's concept of pollution behaviour and the linguist, philosopher, psychoanalyst and feminist Julia Kristeva's concept of the abjective to explain and make sense of the findings. Results The findings indicate that the labour performed by the researchers in the trial work involves transforming the foetal material practically, as well as culturally, from trash to treasure. The transformation process contains different phases, and in the interview material we observed that the foetal material or cells were considered objects, subjects or rejected as abject by the researchers handling them, depending on what phase of process or practice they referred to or had experience of. As demonstrated in the analysis, it is the human origin of the cell that makes it abjective and activates pollution discourse, when the researchers talk of their practice. Conclusions The marginal and ambiguous status of the embryo that emerges in the accounts turns the scientists handling foetal cells into liminal characters in modern medicine. Focusing on how practical as well as emotional and cultural strategies and rationalizations of the researchers emerge in interview accounts, this study adds insights on the rationale of practically procuring, transforming and utilizing the foetal material to the already existing studies focused on the donations. We also discuss why the use and refinement of a tissue, around which there is practical consensus but cultural ambiguity, deserves further investigation.
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- Trigo, João Pedro, 1995, et al.
(författare)
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Mild blanching prior to pH-shift processing of Saccharina latissima retains protein extraction yields and amino acid levels of extracts while minimizing iodine content
- 2023
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Ingår i: Food Chemistry. - : Elsevier BV. - 0308-8146 .- 1873-7072. ; 404
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Tidskriftsartikel (refereegranskat)abstract
- The seaweed Saccharina latissima is often blanched to lower iodine levels, however, it is not known how blanching affects protein extraction. We assessed the effect of blanching or soaking (80/45/12 °C, 2 min) on protein yield and protein extract characteristics after pH-shift processing of S. latissima. Average protein yields and extract amino acid levels ranked treatments as follows: blanching-45 °C ∼ control > soaking ∼ blanching-80 °C. Although blanching-45 °C decreased protein solubilization yield at pH 12, it increased isoelectric protein precipitation yield at pH 2 (p < 0.05). The former could be explained by a higher ratio of large peptides/proteins in the blanched biomass as shown by HP-SEC, whereas the latter by blanching-induced lowering of ionic strength, as verified by a dialysis model. Moreover, blanching-45 °C yielded a protein extract with 49 % less iodine compared with the control extract. We recommend blanching-45 °C since it is effective at removing iodine and does not compromise total protein extraction yield.
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- Munthe, Christian, 1962
(författare)
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Precautionary Principle
- 2013
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Ingår i: International Encyclopedia of Ethics. - Chichester : Blackwell. - 9781444367072 ; , s. 4031-4039
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Bokkapitel (refereegranskat)abstract
- Following the statement in the United Nations' Rio Declaration of 1992 that countries should apply a “precautionary approach” in policymaking on environmental and technological issues, the notion of a precautionary principle (PP) has gained ground in worldwide policymaking and regulation, thus catching the interest of ethics scholars. Although seldom explained in much detail, and resulting in quite different policy results in different countries and areas (O'Riordan et al. 2001; Sandin 1999; Trouwborst 2002; Zander 2010), PP is generally understood as a norm urging or permitting policymakers to take preventive action in the face of unknown, uncertain, or probable dangers, motivated by the experience of how seemingly valuable and promising practices may lead to seriously adverse consequences in spite of lack of solid evidence to this effect (Sandin 1999). In ethics debate, this idea has been applied not only to matters regarding the large-scale introduction and use of technology (e.g., regarding energy production, transport and communication, nano- or biotechnology, and so on) (see Biotechnology; Nanotechnology, Ethics of) with possible ensuing impact on the natural environment connecting to the notion of sustainability (see Sustainability), but also, for example, to abortion (see Abortion), medical genetics, embryo experimentation (see Embryo Research), the treatment of animals (see Animal Experimentation), terrorism (see Terrorism), and general research ethics (see Research Ethics) (Munthe 2011). Many of these applications advocate strong conclusions in spite of the fact that PP or its normative justification have not been made very clear. At the same time, PP has been the subject of criticism, much of which boils down to three points: lack of clarity, lack of practicality, and/or ethical implausibility. Curiously, these critical points have often been made in conjunction, in spite of the fact that a clear sense of what PP means seems necessary for backing up the other two objections.
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- Teixeira, Cristina, 1986, et al.
(författare)
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Application of a dynamic gastrointestinal in vitro model combined with a rat model to predict the digestive fate of barley dietary fibre and evaluate potential impact on hindgut fermentation
- 2017
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Ingår i: Bioactive Carbohydrates and Dietary Fibre. - : Elsevier BV. - 2212-6198. ; 9, s. 7-13
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Tidskriftsartikel (refereegranskat)abstract
- Processing affects the composition, structure and physico-chemical characteristics of dietary fibres, and further changes can occur along the gastro-intestinal tract before entering the colon where undigested carbohydrates may serve as a substrate for the microbiota. To elucidate the effects of dietary fibre characteristic and the impact of processing on the digestive fate of dietary fibre components, in the stomach and the small intestine, a dynamic gastrointestinal in vitro model (TIM-1) was used. Three barley malt and one brewers’ spent grain, with different contents of soluble fibre, arabinoxylan (total and soluble), and β-glucan with varying Calcofluor average molecular weight (Mcf) and molecular weight distribution were evaluated in the in vitro model. Additionally, a rat model was used to study the colonic fermentation of the dietary fibre components in the barley products. The transit time through the stomach and small intestine of the in vitro model was slowest for barley malts with the highest content of soluble fibre and β-glucan. The β-glucan Mcf decreased with digestion time for all test meals, and the molecular weight distributions in the various barley products differed markedly. The highest β-glucan Mcf was found for Cinnamon malt, which also contained the highest proportion of soluble fibre. The hindgut fermentation of fibre and the proportions of caecal propionic acid were highest in rats fed barley products high in soluble fibre, β-glucan (content and Mcf), soluble arabinoxylan, and with slowest transit during digestion in the in vitro model.
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- Palsdottir, Vilborg, 1979, et al.
(författare)
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Postnatal deficiency of essential fatty acids in mice results in resistance to diet-induced obesity and low plasma insulin during adulthood
- 2011
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Ingår i: Prostaglandins, Leukotrienes and Essential Fatty Acids. - : Elsevier BV. - 1532-2823 .- 0952-3278. ; 84:3-4, s. 85-92
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Tidskriftsartikel (refereegranskat)abstract
- Our objective was to investigate the long-term metabolic effects of postnatal essential fatty acid deficiency (EFAD). Mouse dams were fed an EFAD diet or an isoenergetic control diet 4 days before delivery and throughout lactation. The pups were weaned to standard diet (STD) and were later subdivided into two groups: receiving high fat diet (HFD) or STD. Body composition, energy expenditure, food intake and leptin levels were analyzed in adult offspring. Blood glucose and plasma insulin concentrations were measured before and during a glucose tolerance test. EFAD offspring fed STD were leaner with lower plasma leptin and insulin concentrations compared to controls. EFAD offspring fed HFD were resistant to diet-induced obesity, had higher energy expenditure and lower levels of plasma leptin and insulin compared to controls. These results indicate that the fatty acid composition during lactation is important for body composition and glucose tolerance in the adult offspring.
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- Johansson, Daniel, et al.
(författare)
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Impact of food processing on rye product properties and their in vitro digestion
- 2018
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Ingår i: European Journal of Nutrition. - : Springer Science and Business Media LLC. - 1436-6207 .- 1436-6215. ; 57:4, s. 1651-1666
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Tidskriftsartikel (refereegranskat)abstract
- Rye products have been reported to elicit postprandial insulin and glucose responses which may be beneficial for prevention of type-2 diabetes. However, mechanisms underlying variations in responses related to processing techniques are not fully understood.
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