| 1. |
- Jesorka, Aldo, 1967, et al.
(författare)
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Microfluidic technology for investigation of protein function in single adherent cells
- 2019
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Ingår i: Methods in Enzymology. - : Elsevier. - 1557-7988 .- 0076-6879. ; 628
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Instrumental techniques and associated methods for single cell analysis, designed to investigate and measure a broad range of cellular parameters in search of unique features, address key limitations of conventional cell-based assays with their ensemble average response. While many different single cell techniques exist for suspension cultures, which can process and characterize large numbers of individual cells in rapid succession, the access to surface-immobilized cells in typical 2D and 3D culture environments remains challenging. Open space microfluidics has created new possibilities in this area, allowing for exclusive access to single cells in adherent cultures, even at high confluency. In this chapter, we briefly review new microtechnologies for the investigation of protein function in single adherent cells, and present an overview over related recent applications of the multifunctional pipette (Biopen), a microfluidic multi-solution dispensing system that uses hydrodynamic confinement in open volume environments in order to establish a superfusion zone over selected single cells in adherent cultures.
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| 2. |
- Wasén, Kristian, 1980, et al.
(författare)
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The Visual Touch Regime: Real-Time 3D Image-Guided Robotic Surgery and 4D and ‘5D’ Scientific Visualisation at Work.
- 2013
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Ingår i: Emerging health technology : relocation of innovative visual knowledge and expertise / Kristian Wasén, editor. - Heidelberg : Springer. - 9783642325694
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Bokkapitel (refereegranskat)abstract
- Emerging multidimensional imaging technologies (3D/4D/”5D”) open new ground for exploring visual worlds and rendering new image-based knowledge, especially in areas related to medicine and science. 3D imaging is defined as three visual dimensions. 4D imaging is three visual dimensions plus time. 4D imaging can also be combined with functional transitions (i.e., following radioactive tracer isotope through the body in positron emission tomography). 4D imaging plus functionality is defined as “5D” imaging. We propose the idea of “visual touch”, a conceptual middle ground between touch and vision, as a basis for future explorations of contemporary institutional standards of image-based work. “Visual touch” is both the process of reconciling the senses (human and artificial) and the end result of this union of the senses. We conclude that while new multi-dimensional imaging technology emphasises vision, new forms of image-based work using visual materials cannot solely be classified as “visual”.
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| 3. |
- Campbell, Kate, 1987, et al.
(författare)
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Self-Establishing Communities: A Yeast Model to Study the Physiological Impact of Metabolic Cooperation in Eukaryotic Cells
- 2019
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer New York. - 1940-6029 .- 1064-3745. ; 2049, s. 263-282
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- All biosynthetically active cells are able to export and import metabolites, the small molecule intermediaries of metabolism. In dense cell populations, this hallmark of cells results in the intercellular exchange of a wide spectrum of metabolites. Such metabolite exchange enables metabolic specialization of individual cells, leading to far reaching biological implications, as a consequence of the intrinsic connection between metabolism and cell physiology. In this chapter, we discuss methods on how to study metabolite exchange interactions by using self-establishing metabolically cooperating communities (SeMeCos) in the budding yeast Saccharomyces cerevisiae. SeMeCos exploit the stochastic segregation of episomes to progressively increase the number of essential metabolic interdependencies in a community that grows out from an initially prototrophic cell. By coupling genotype to metabotype, SeMeCos allow for the tracking of cells while they specialize metabolically and hence the opportunity to study their progressive change in physiology.
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| 4. |
- Wasén, Kristian, 1980
(författare)
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Introduction
- 2013
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Ingår i: Emerging Health Technology: Relocation of Innovative Knowledge and Expertise. / Kristian Wasén, editor. - Heidelberg : Springer. - 9783642325694
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Bokkapitel (refereegranskat)abstract
- The opening chapter addresses the different ways in which visual knowledge and medical health expertise are being reorganised and relocated and thereby reinforced and disputed in new practices of professional work involving state-of-the-art health technology. Change management and related concepts are discussed and defined. The chapter shows that emerging health technologies, organisational renewal, and the discovery of unexpected innovative knowledge are highly interconnected in professional practice. Emerging health technology refers both to new work, physical devices, and visual knowledge and expertise that, when located and relocated in the institutional landscape—or combined and recombined in health practices—may unfold unexpectedly into new patient-doctor constellations and organisational structures.
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| 5. |
- Pless, Christian J., et al.
(författare)
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Emerging strategies in 3D printed tissue models for in vitro biomedical research
- 2022
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Ingår i: Bioprinting : From Multidisciplinary Design to Emerging Opportunities - From Multidisciplinary Design to Emerging Opportunities. - 9780323854306 - 9780323854313 ; , s. 207-246
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Bokkapitel (refereegranskat)abstract
- 3D bioprinting has the potential to provide a unified framework for the manufacturing of tissue models for biomedical research, including drug discovery, disease modeling, and regenerative medicine. However, it remains challenging to 3D print replicas of human tissues that have accurate cell types, cellular densities, extracellular matrix compositions, and that can be assayed in a minimally invasive manner for chronic studies. Here, we review how recent breakthroughs in stem cell biology, tissue engineering, and materials science have led to novel 3D printing strategies that have the potential to solve these challenges.
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| 6. |
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| 7. |
- Bergman, Alexandra Linda, 1985, et al.
(författare)
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Metabolic Engineering Strategies to Convert Carbohydrates to Aviation Range Hydrocarbons
- 2016
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Ingår i: Biofuels for Aviation: Feedstocks, Technology and Implementation. - 9780128045688 ; , s. 151-190
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Numerous hydrocarbon molecules with properties similar to aviation fuels can be found in nature, but are often only produced at low quantities in their native hosts. This chapter summarizes recent progress in the engineering of microbial cell factories for the production of the two major molecule classes: fatty acid-derived alkanes/alkanes and isoprenoids. It starts with a brief introduction to metabolic engineering. For each of the two molecule classes, it provides information on biosynthetic pathways as well as engineering strategies to enhance their formation in the microbial hosts. While most of these endeavours are still at the proof-of concept stage, the first commercial examples are beginning to emerge.
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| 8. |
- Li, Yan, et al.
(författare)
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Osseointegrated amputation prostheses and implanted electrodes
- 2021
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Ingår i: Bionic Limb Reconstruction. - Cham : Springer International Publishing. ; , s. 45-55
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- The load transfer from the external prosthesis to the residual limb via the socket can cause significant stress on the soft tissues, leading to irritation and skin ulcers. Osseointegrated bone-anchored prostheses systems create a direct structural and functional connection between the prosthesis and residual skeleton. Up to date, standardized implant systems, surgical techniques, and postoperative rehabilitation protocols have been developed for osseointegrated prostheses for the rehabilitation of amputees (OPRA), which has resulted in better functionality, fewer complications, and a better quality of life for implant recipients. The OPRA implant systems can now incorporate neuromuscular electrodes to facilitate myoelectric control and sensory feedback, which is especially important for upper extremity amputees. The latest development, called the osseointegrated human-machine gateway, allows for permanent implantation of neuromuscular electrodes, which provide long-term stable signals for myoelectric control, independent of limb position or environmental conditions, as well as artificial sensory feedback. In addition, the modular design of this system allows any part to be upgraded or replaced with minimal disturbance to the other components. The osseointegrated implants and the human-machine gateway represent frontiers in amputee rehabilitation.
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| 9. |
- Löfdahl, Lennart, 1948, et al.
(författare)
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Sensors and actuators for turbulent flows
- 2005
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Ingår i: MEMS: Applications. - 9781420036558 ; , s. 6-1-6-77-
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- During the last decade MEMS devices have had major impacts on both industrial and medical applications. Examples of the former group are accelerometers for automobile airbags, scanning electron microscope tips to image single atoms, microheatexchangers for electronic cooling, and micromirrors used for light-beam steering. Micropumping is useful for ink-jet printing and cooling of electronic equipment. Existing and prospective medical applications include reactors for separating biological cells, controlled delivery and measurement of minute amount of medication, pressure sensors for catheter tips, and development of an artificial pancreas. The concept of MEMS includes a variety of devices, structures, and systems: this chapter will focus only on MEMS devices that generally can be categorized as microsensors and microactuators. Particularly, attention is directed toward microdevices used for turbulent-flow diagnosis and control.
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| 10. |
- von Zur-Mühlen, Bengt, Docent, 1966-, et al.
(författare)
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Treating diabetes with islet transplantation : Lessons learnt from the Nordic network for clinical islet transplantation
- 2019. - 1
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Ingår i: Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas. - : Academic Press. - 9780128148334 ; , s. 599-611
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Bokkapitel (refereegranskat)abstract
- In this chapter we present the Nordic experiences withclinical islet transplantation after starting networks. TheNordic Network for Clinical Islet Transplantation (NNCIT)was established in 2000. In addition, we also present a localmultidisciplinary effort organized as part of a Centerof Excellence (CoE) in Type 1 Diabetes. Even though careof type 1 diabetic patients has improved over time thereare still patients who have substantial problems withhypoglycemic incidents, unawareness, and glycemiclability and need treatment other than injected exogenousinsulin.1 In the Nordic countries it is now consideredas innovative rather than an experimental therapy.Islet transplantation still has limitations, only making ita treatment suitable for a small part of type 1 diabeticpatients. The main limitations are the need of immunosuppressanttherapy (as in all organ transplants betweenindividuals), early loss of substantial amounts of transplantedislets, and a limited source of islets (deceasedorgan donors). To have a favorable clinical result there isoften a need for retransplantation and or complementarytherapies. Islet transplantation as such has great potentialin the near future for modification, encapsulation ofthe islets, or stem cell therapies.2,3 Experiences made sofar will be useful when this new era evolves. Even if islettransplantation is a minimally invasive and surgicallysimple technique there are technically complex partssuch as islet isolation that require highly specialized staffand a need for multidisciplinary expertise, which is whyit for the foreseeable future will continue to be centralizedto a few units to ensure high quality and continueddevelopment. The aim of our collaborations, local,regional, and international, is to offer the best treatmentfor those patients for whom conventional treatment isinadequate and take advantage of the expertise distributedin several different disciplines.
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