| 2. |
- Encarnacao, Joao Crispim, et al.
(författare)
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Impact of assay temperature on antibody binding characteristics in living cells : A case study
- 2017
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Ingår i: BIOMEDICAL REPORTS. - : Spandidos Publications. - 2049-9434 .- 2049-9442. ; 7:5, s. 400-406
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Tidskriftsartikel (refereegranskat)abstract
- Kinetic and thermodynamic studies of ligand-receptor interactions are essential for increasing the understanding of receptor activation mechanisms and drug behavior. The characterization of molecular interactions on living cells in real-time goes beyond most current binding assays, and provides valuable information about the dynamics and underlying mechanism of the molecules in a living system. The effect of temperature on interactions in cell-based assays is, however, rarely discussed. In the present study, the effect of temperature on binding of monoclonal antibodies, cetuximab and pertuzumab to specific receptors on living cancer cells was evaluated, and the affinity and kinetics of the interactions were estimated at selected key temperatures. Changes in the behavior of the interactions, particularly in the on- and off-rates were observed, leading to greatly extended time to reach the equilibrium at 21 degrees C compared with at 37 degrees C. However, the observed changes in kinetic characteristics were less than a factor of 10. It was concluded that it is possible to conduct real-time measurements with living cells at different temperatures, and demonstrated that influences of the ambient temperature on the interaction behavior are likely to be less than one order of magnitude.
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| 3. |
- Hagsten, Kenneth, et al.
(författare)
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Self-propelled Employees - Co-workership in Swedish Community Pharmacies
- 2024
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Ingår i: Nordic Journal of Working Life Studies. - : Roskilde Universitetsforlag. - 2245-0157. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- In this article, work in Swedish pharmacies is examined. With the Nordic concept of co-workership as a frame, the aim is to examine how employees in pharmacies view and handle their work situations and the reasons behind them. Data were mainly collected with 20 qualitative semistructured interviews in five different pharmacies. The data were organized and analyzed by using the content analysis process. In the studied pharmacies, it can be summarized as work was carried out in a self-propelled way. Employees take an overall responsibility for the workplace, have ambition to help others and to be a part of a good team, while most of the work is carried out individually and independently.
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