| 1. |
- Forchheimer, Daniel, et al.
(författare)
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Molecularly selective nanopatterns using nanoimprint lithography : A label-free sensor architecture
- 2011
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Ingår i: Journal of Vacuum Science and Technology B. - : AVS Science and Technology Society. - 2166-2746 .- 2166-2754. ; 29:1
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Tidskriftsartikel (refereegranskat)abstract
- Nanoimprint lithography (NIL) can generate well defined nanostructures with high efficiency and at very low cost. Molecular imprinting (MIP) is a "bottom-up" technique creating a polymer layer exhibiting structures with a molecular selectivity. Such polymer structures may be employed as molecular recognition sites for sensing applications. In this work, the authors combine NIL with MIP and they are able to obtain micro- and nanopatterns of polymer with features down to 100 nm that show high molecular selectivity.
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| 2. |
- Zhou, Tongchang, et al.
(författare)
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Molecularly Imprinted Polymer Beads Prepared by Pickering Emulsion Polymerization for Steroid Recognition
- 2014
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Ingår i: Journal of Applied Polymer Science. - : Wiley. - 1097-4628 .- 0021-8995. ; 131:1, s. 39606-39606
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Tidskriftsartikel (refereegranskat)abstract
- Pickering emulsion polymerization was used to synthesize molecularly imprinted polymer beads for the selective recognition of 17-beta-estradiol under aqueous conditions. Scanning electron microscopy analysis indicated that the imprinted polymer beads had a small diameter with a narrow size distribution (18.9 +/- 2.3 mu m). The reduction in particle size achieved in this study was attributed to the altered polarity of the stabilizing nanoparticles used in the Pickering emulsion. The imprinted polymer beads could be used directly in water and showed a high binding affinity for the template molecule, 17-beta-estradiol, and its structural analogs. These water-compatible polymer beads could be used as affinity adsorbents for the extraction and analysis of low-abundance steroid compounds in aqueous samples. (C) 2013 Wiley Periodicals, Inc.
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| 3. |
- Chaudhary, Shilpi, et al.
(författare)
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Controlled short-linkage assembly of functional nano-objects
- 2014
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Ingår i: Applied Surface Science. - : Elsevier BV. - 1873-5584 .- 0169-4332. ; 300, s. 22-28
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Tidskriftsartikel (refereegranskat)abstract
- In this work, we report a method that allows the deterministic, photo-controlled covalent assembly of nanoparticles directly on surface. As a model system, we study the conjugation of molecularly imprinted polymer (MIP) nanoparticles on a glass surface and confirm that the immobilized nanoparticles maintain their molecular recognition functionality. The glass slide was first modified with perfluorophenylazide and then used to bind MIP nanoparticles under UV irradiation. After each step the surface was analyzed by water contact angle measurement, fluorescence microscopy, scanning electron microscopy, and/or synchrotron-based X-ray photoelectron spectroscopy. The MIP nanoparticles immobilized on the glass surface remained stable and maintained specific binding for the template molecule, propranolol. The method developed in this work allows MIP nanoparticles to be directly coupled to a flat surface, offering a straightforward means to construct robust chemical sensors. Using the reported photo conjugation method, it is possible to generate patterned assembly of nanoparticles using a photomask. Since perfluorophenylazide-based photochemistry works with all kinds of organic material, the method developed in this work is expected to enable immobilization of not only MIPs but also other kinds of organic and inorganic-organic core-shell particles for various applications involving photon or electron transfer. (C) 2014 The Authors. Published by Elsevier B.V. All rights reserved.
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| 4. |
- Ye, Lei
(författare)
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Artificial receptors: New opportunities for the exploitation of molecularly imprinted polymers
- 1999
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Molecular imprinting, method for creating artificial receptors that are mimics of biological antibodies and receptors, is based on the concept of creating substrate-specific recognition sites in polymeric matrices by means of template polymerisation. The polymeric receptors produced display favourable binding characteristics, such as high affinity and specificity towards the substrate molecules. Compared to natural antibodies and receptors, imprinted polymers are much more stable and can therefore be utilised under considerably harsher conditions. In the researches described in this thesis, non-covalent interactions are the major force driving the assembly of imprinted receptors. Given that non-covalent molecular interactions dominate in all biological recognition processes, their application in molecular imprinting is in principle unlimited. The general applicability of the non-covalent approach for the imprinting of various substrate molecules is reflected in the increasing number of publications in recent years. Non-covalent molecularly imprinted polymers have been applied in many areas, for example as stationary phases in chiral separation, as affinity adsorbents for sample pre-treatment using solid-phase extraction, as antibody mimics for drug determination, etc. Summarised in this thesis are novel applications for molecularly imprinted polymers prepared via the non-covalent approach. Artificial antibodies against corticosteroids have been prepared that exhibit binding behaviour similar to their natural counterparts. The utility of imprinted polymers for the screening of combinatorial chemical libraries is demonstrated, where the compounds of interest can be specifically isolated from libraries composed of many similar structures. As specific adsorbents, molecularly imprinted polymers are used for product purification following chemical synthesis and for in situ product removal during a biotransformation process to enhance product yield. Finally, a new imprinting methodology is developed for the production of affinity microspheres that have various applications in analytical and medical sciences.
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| 5. |
- Gong, Haiyue, et al.
(författare)
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Dynamic assembly of molecularly imprinted polymer nanoparticles
- 2018
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Ingår i: Journal of Colloid and Interface Science. - : Elsevier BV. - 0021-9797. ; 509, s. 463-471
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Tidskriftsartikel (refereegranskat)abstract
- Manipulation of specific binding and recycling of materials are two important aspects for practical applications of molecularly imprinted polymers. In this work, we developed a new approach to control the dynamic assembly of molecularly imprinted nanoparticles by surface functionalization. Molecularly imprinted polymer nanoparticles with a well-controlled core-shell structure were synthesized using precipitation polymerization. The specific binding sites were created in the core during the first step imprinting reaction. In the second polymerization step, epoxide groups were introduced into the particle shell to act as an intermediate linker to immobilize phenylboronic acids, as well as to introduce cis-diol structures on surface. The imprinted polymer nanoparticles modified with boronic acid and cis-diol structures maintained high molecular binding specificity, and the nanoparticles could be induced to form dynamic particle aggregation that responded to pH variation and chemical stimuli. The possibility of modulating molecular binding and nanoparticle assembly in a mutually independent fashion can be exploited in a number of applications where repeated use of precious nanoparticles is needed.
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| 6. |
- Schanzenbach, Christoph, et al.
(författare)
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Preparation and characterisation of a sensing system for wireless pH measurements in vivo, in a rumen of a cow
- 2017
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Ingår i: Sensors and actuators. B, Chemical. - : Elsevier BV. - 0925-4005 .- 1873-3077. ; 242, s. 637-644
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Tidskriftsartikel (refereegranskat)abstract
- We describe a sensing system that is able to measure pH in-vivo, in the rumen of a cow, in real time. The sensing principle is based on gravimetric transduction using a magnetoelastic ribbon functionalized by pH-sensitive nanobeads that is placed in the rumen where it is actuated and read-out wirelessly. We describe a generic procedure that enables one to deposit monolayers or multilayers of nano- and micro beads onto virtually any substrate. The topography of the resulting layers as well as interlayer coverages were characterised using optical microscopy and scanning profilometry. First we determined performance of the system in-vitro, in phosphate-buffered saline, in McDougall's buffer and in a rumen fluid. Thereafter we also performed in-vivo measurements. Using buffers we determined pH response in the liquids both at the fundamental frequency of the functionalised foils, and at the 1st overtone. We argue that observed frequency changes vs pH are mainly due to changes of trapped liquid when the bead layers shrink or expand as a response to changed pH. The data obtained from the pH response of magnetoelastic foils at different bead coverages was modelled by a simple two-parameter model that corroborates this assumption.
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| 7. |
- Isarankura-Na-Ayudhya, Chartchalerm, et al.
(författare)
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Computational Insights on Sulfonamide Imprinted Polymers
- 2008
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Ingår i: Molecules. - : MDPI AG. - 1420-3049. ; 13:12, s. 3077-3091
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Tidskriftsartikel (refereegranskat)abstract
- Molecular imprinting is one of the most efficient methods for preparing synthetic receptors that possess user defined recognition properties. Despite general success of non-covalent imprinting for a large variety of templates, some groups of compounds remain difficult to tackle due to their structural complexity. In this study we investigate preparation of molecularly imprinted polymers that can bind sulfonamide compounds, which represent important drug candidates. Compared to the biological system that utilizes metal coordinated interaction, the imprinted polymer provided pronounced selectivity when hydrogen bond interaction was employed in an organic solvent. Computer simulation of the interaction between the sulfonamide template and functional monomers pointed out that although methacrylic acid had strong interaction energy with the template, it also possessed high non-specific interaction with the solvent molecules of tetrahydrofuran as well as being prone to self-complexation. On the other hand, 1-vinylimidazole was suitable for imprinting sulfonamides as it did not cross-react with the solvent molecules or engage in self-complexation structures.
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| 8. |
- Trikka, Foteini A., et al.
(författare)
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Molecularly imprinted polymers for histamine recognition in aqueous environment
- 2012
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Ingår i: Amino Acids. - : Springer Science and Business Media LLC. - 0939-4451 .- 1438-2199. ; 43:5, s. 2113-2124
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Tidskriftsartikel (refereegranskat)abstract
- Molecularly imprinted polymers (MIP) for histamine using methacrylic acid were developed and recognition mechanisms were thoroughly characterized for the first time in this study. The binding affinity of imprinted polymer with structurally related compounds was studied in organic and aqueous media, at various conditions. In organic media, MIP was found to bind histamine two and six times more than ranitidine and fluoxetine, respectively, whereas higher selectivity was observed in the case of dimentidene or disodium cromoglycate. The specific binding sites of MIP recognized histamine over l-histidine in aqueous conditions, while higher affinity for histamine compared to ranitidine, disodium cromoglycate, putrescine and to a putrescine analogue was observed. A combination of NMR and UV spectroscopy analyses for investigation of imprinting and recognition properties revealed that strong specific interactions between the functional monomer and histamine in the prepolymerization and in the aqueous solutions were probably responsible for histamine recognition. The preparation of histamine MIPs and elucidation of imprinting and recognition mechanism may serve as useful insight for future application of MIPs.
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| 9. |
- Ye, Lei, et al.
(författare)
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Molecularly imprinted materials: Towards the next generation
- 2002
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Ingår i: Materials Research Society Symposium - Proceedings. - 0272-9172. ; 723, s. 51-59
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Konferensbidrag (refereegranskat)abstract
- This brief overview summarizes some recent developments from our Center for Molecular Imprinting related to the topic of this symposium. After a short presentation of the principle of molecular imprinting and recognition, the use of different materials including hybrids for the formation of the host will be discussed, followed by examples given of different formats used such as small polymer beads. In closing, potential directions for the next generation of molecular imprinting technology will be discussed.
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| 10. |
- Zhang, Qicheng, et al.
(författare)
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Molecularly Imprinted Polymers for Targeting Lipopolysaccharides and Photothermal Inactivation of Pseudomonas aeruginosa
- 2023
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Ingår i: ACS Applied Polymer Materials. - 2637-6105. ; 5:4, s. 3055-3064
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Tidskriftsartikel (refereegranskat)abstract
- Although photothermal therapy is of significance in therapeutic strategies for fighting bacterial infection, the precise target of photothermal agents to bacterial sites is still a challenge. In this work, lipopolysaccharide (LPS) imprinted photothermal molecularly imprinted polymers (PMIP) were prepared for the efficient capture and elimination of Pseudomonas aeruginosa. The LPS derived from Pseudomonas aeruginosa was selected as a template due to its cis-diol structure, which can provide active sites to direct the boronate affinity-mediated synthesis of molecularly imprinted polymers. Polydopamine with good biocompatibility and photothermal effect was used as an imprinting matrix to achieve good photothermal function and imprinting efficiency. The combination of bacteria-imprinting with photothermal ability allowed PMIP to deactivate target bacteria with enhanced precision and efficiency. Taken together, our study offers a promising strategy to design synthetic materials for targeting and treating pathogens for various infectious diseases and expands the application of molecular imprinting technology in the field of antimicrobials.
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