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- Xie, Bin
(creator_code:cre_t)
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[An optical circulation amplification type biological chip detecting process]
- 2004
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Patent (övrigt vetenskapligt/konstnärligt)abstract
- The invention discloses an optical circulation enlarged type biological chip detecting method, wherein the enzyme recirculation and bioluminescence are combined, for realizing the FMNH#-[2] accumulation through the circulation on the electrodes by the NADH/NAD#+[+] oxidation-reduction, the high level accumulation FMNH#-[2] reacts with other light-emitting bottom luciferase, long chain aldehydes and oxygen, giving off fluorescent light having wavelength of 490nm. The invention realizes high sensibility, low cost and simplicity of operation, thus is suitable for each types of biological sample analysis.
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| 3. |
- Xie, Bin
(creator_code:cre_t)
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氨酰基转运核糖核酸合成酶识别型蛋白芯片的制备方法
- 2004
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Patent (övrigt vetenskapligt/konstnärligt)abstract
- A method of manufacturing a protein chip for identifying aminoacyl-tRNA synthetases, suitable for detecting basic amino acids in proteins. It is characterized in, immoblizing aminoacyl-tRNA synthetases corresponding to 20 basic amino acids in arrays onto the surface of the chip, then fixing 20 kinds of tRNAs labelled by fluorescence onto the corresponding synthetases, then obtaining a chip of aminoacyl-tRNA synthetases. The chip could detect amino acids in proteins. By scanning fluorescence, amino acids in proteins will be determined based on fluorescence of 20 wells.
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| 4. |
- Fu, L., et al.
(författare)
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Wear mechanism of Cr 3 C 2 /Ni 3 Al composites showing excellent wear resistance
- 2019
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Ingår i: Gongcheng Kexue Xuebao/Chinese Journal of Engineering. - 2095-9389. ; 41:1, s. 117-123
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Tidskriftsartikel (refereegranskat)abstract
- The Ni 3 Al intermetallic compound is considered an excellent wear-resistant material. The addition of Cr 3 C 2 particles can further improve the wear resistance of Ni 3 Al-based alloys. In order to elucidate the wear mechanism of Cr 3 C 2 /Ni 3 Al composites improved by the Cr 3 C 2 strengthening phase, Ni 3 Al-alloy and Cr 3 C 2 /Ni 3 Al composites were prepared by the hot isostatic pressing process in this study. The mechanical properties and wear resistance of each phase in the Ni 3 Al-alloy and Cr 3 C 2 /Ni 3 Al composites were investigated using a nano-indentation instrument and a pin-on-disk friction and wear tester, respectively. The worn surface morphologies and the hardness of the subsurface layer under the worn surfaces of the Ni 3 Al-alloy and Cr 3 C 2 /Ni 3 Al composites were determined by a scanning electron microscopy (SEM) and a nano-indentation instrument. The results indicate that the hardness of the matrix phase in the Cr 3 C 2 /Ni 3 Al composites is significantly improved by the addition of Cr 3 C 2 particles. The nano-hardness and the elastic modulus of each phase in the Cr 3 C 2 /Ni 3 Al composites gradually increase from matrix phase through diffusion phase to hard core phase. The mechanical properties between the matrix, diffusion, and hard core phases in the Cr 3 C 2 /Ni 3 Al composites present a gradient transition. This kind of structure distribution is good for enhancing the wear resistance of Cr 3 C 2 /Ni 3 Al composite materials. As for friction and wear conditions in this study, abrasive wear was the dominant wear mechanism, which occurred on the surfaces of the Ni 3 Al-alloy and Cr 3 C 2 /Ni 3 Al composites. The Cr 3 C 2 /Ni 3 Al composites showed a good wear resistant property. The carbide-strengthening phase can block up the cutting action of the wear debris, reduce the interaction between the wear materials, and decrease the thickness of the subsurface layer and the size of the wear debris, resulting in improved wear resistance of Cr 3 C 2 /Ni 3 Al composites.
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| 6. |
- Liang, Zhen-pu, et al.
(författare)
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单分子层修饰生物芯片基片的电化学研究
- 2005
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Ingår i: Journal of Wuhan University (Natural Science Edition). - 1671-8836. ; :2, s. 249-252
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Tidskriftsartikel (refereegranskat)abstract
- A biotin-avidin modified biochip has been constructed, and its electrochemistry characteristics were studied with K4Fe(CN)(6).3H2O and ferrocene as the indicators by cyclic voltammograms. Electrochemical experiments showed that this biochip has high response signal, reproducibility,uniformity and low background signal; the ferrocene can be used as high efficiency electron medium. And these results were discussed in this paper.
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| 7. |
- Zhang, Huichun, et al.
(författare)
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Wind tunnel experiment and regression model for spray drift
- 2015
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Ingår i: Transactions of the Chinese Society of Agricultural Engineering. - Beijing, China : Chinese Society of Agricultural Engineering. - 1002-6819. ; 31:3, s. 94-100
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Tidskriftsartikel (refereegranskat)abstract
- With greater environmental awareness, the movement of pesticides within and off of a spray target area is acritical public concern. Ideally, all of the material applied should be deposited within the targeted swath on the intendedpest or plant. But realistically, a portion of the spray remains airborne and is carried downwind to non-target areas.Airborne spray leaving the targeted area reduces the applied dosage, and could cause damage to neighboring plant andwater source or other detrimental environmental impacts. To study the influences of nozzle type, spray mixture and windspeed on spray drift, experiments were conducted using a wind tunnel. Spray drift risk was assessed by adding a tracer tothe spray mixture and measuring the quantities of spray deposited downwind from the nozzle on horizontal polythenelines with 2 mm diameter perpendicular to the wind direction in a vertical and a horizontal array. At a distance of 2 mdownwind from the static nozzle, five collector lines (V1 to V5) were positioned one above the other at the spacing of0.1 m to provide an estimate of the spray still airborne through this vertical profile. An additional five sampling collectorstrings (H1 to H5) were placed in a horizontal array with one-meter horizontal spacing at 0.1 m height to determine thefallout volumes and gradients of the spray from 2 to 6 m downwind. A water-soluble fluorescent tracer was dissolvedinto tap water as the spray liquid, and after the experiments, the collecting lines were washed with deionized water tomeasure deposit and drift. The results indicated that deposits on sampling collector decreased with increased verticalelevation and horizontal distance. Average fallout and airborne deposit resulting from the different spray applicationswere shown in the paper. These figures showed the expected fallout and airborne profiles for all tested nozzle types andsizes. The highest fallout deposits were measured at a position closest to the nozzle (H1) with a systematic decrease withthe distance from the nozzle. The highest airborne deposits were found at the lowest sampling collector (V1) with asystematic decrease with increasing height above the wind tunnel floor. Airborne spray drift was affected by wind speed.At all sample positions, deposits on collectors were reduced at lower wind speed. Nozzle’s structure was also found toinfluence droplet’s size, so injector/pre-orifice nozzle produced coarser droplets and reduced spray drift. The amount ofspray recovered is based on the amount of active ingredient of spray mixture within each droplet rather than the totaldroplet volume. On that basis, a multiple non-linear model for statistical drift prediction including four independent,non-correlated variables (target distance, wind speed, nozzle type and chemical type) was established. The regressionmodel provided a drift evaluation approach, and it was important in the interpretation of wind tunnel data for differentnozzle types, chemical types and sampling methodologies.
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