| 1. |
- Gunnarsson, Lina-Maria, 1977, et al.
(författare)
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Comparison of six different sewage treatment processes-Reduction of estrogenic substances and effects on gene expression in exposed male fish
- 2009
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Ingår i: Science of the Total Environment. - : Elsevier. - 0048-9697 .- 1879-1026. ; 407:19, s. 5235-5242
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Tidskriftsartikel (refereegranskat)abstract
- Treated sewage effluents often contain a mixture of estrogenic compounds in low concentrations. The total combined activity of these, however, may be sufficiently high to affect the reproduction of aquatic vertebrates. The introduction of advanced treatment technologies has been suggested as a way to remove micro-contaminants, including estrogenic substances. In this study, one municipal influent was treated with six different processes in parallel on a semi-large scale in order to assess their potential to reduce substances that could contribute to estrogenic effects in male fish. The effluent from a conventional, activated sludge treatment line was compared to a similarly treated effluent with a final sand-filtering step. The addition of ozonation (15 g O-3/m(3)). a moving bed biofilm reactor (MBBR) or both in combination was also evaluated. There was also a separate treatment line that was based on a membrane bioreactor. A small battery of hepatic estrogen-responsive genes was measured in the exposed fish using quantitative PCR. Concentrations of steroid estrogens and estrogenic phenols in the effluents were measured by GC-ECNI-MS. The ozonated effluents were the only tested effluents for which all measured biological effects in exposed fish were removed. Chemical data suggested that the MBBR technology was equally effective in removing the analyzed estrogens; however, elevated expression of estrogen-responsive genes suggested that some estrogenic substances were still present in the effluent. The membrane bioreactor removed most of the measured estrogens and it reduced the induction of the estrogen-responsive genes. However, fish exposed to this effluent had significantly enlarged livers. Given that the same influent was treated in parallel with a broad set of technologies and that the chemical analyses were combined with an in vivo assessment of estrogenic responses, this study provides valuable input into the assessment of advanced treatment processes for removing estrogenic substances.
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| 2. |
- Larsson, Åke, 1944, et al.
(författare)
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Kustfisk - hälsa
- 2012
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Ingår i: HAVET 2012 - Om miljötillståndet i svenska havsområden. - 1654-6741. ; år 2012
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Forskningsöversikt (refereegranskat)
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| 3. |
- Hanson, Niklas, 1976, et al.
(författare)
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Förändringar i fiskhälsa - orsaker söks på bred front
- 2012
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Ingår i: HAVET 2012 - Om miljötillståndet i svenska havsområden. - 1654-6741. ; år 2012, s. 85-87
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Forskningsöversikt (refereegranskat)abstract
- Kustfiskens hälsa har blivit allt mer påverkad under de senaste 20–25 åren. Mycket tyder på att det beror på miljögifter. Samtidigt minskar halterna av de miljögifter som övervakas i samma fiskar. Det tyder på att det är andra, kanske okända, miljögifter som ligger bakom. I ett nytt samverkansprojekt ska man försöka ta reda på orsakerna.
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| 4. |
- Samuelsson, Linda, 1973, et al.
(författare)
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Using NMR metabolomics to identify responses of an environmental estrogen in blood plasma of fish
- 2006
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Ingår i: Aquatic Toxicology (Amsterdam, Netherlands). - : Elsevier BV. - 0166-445X. ; 78:4, s. 341-349
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Tidskriftsartikel (refereegranskat)abstract
- Nuclear magnetic resonance (NMR) based metabolomics in combination with multivariate data analysis may become valuable tools to study environmental effects of pharmaceuticals and other chemicals in aquatic organisms. To explore the usefulness of this approach in fish, we have used H-1 NMR metabolomics to compare blood plasma and plasma lipid extracts from rainbow trout exposed to the synthetic contraceptive estrogen ethinylestradiol (EE2) with plasma from control fish. The plasma metabolite profile was affected in fish exposed to 10 ng/L but not 0.87 ng/L of EE2, which was in agreement with an induced vitellogenin synthesis in the high dose group only, as measured by ELISA. The main affected metabolites were vitellogenin, alanine, phospholipids and cholesterol. The responses identified by this discovery-driven method could be put in context with previous knowledge of the effects of estrogens on fish. This adds confidence to the approach of using NMR metabolomics to identify environmental effects of pharmaceuticals and other contaminants. (c) 2006 Elsevier B.V. All rights reserved.
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