| 1. |
- Culverhouse, R. C., et al.
(författare)
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Collaborative meta-analysis finds no evidence of a strong interaction between stress and 5-HTTLPR genotype contributing to the development of depression
- 2018
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Ingår i: Molecular Psychiatry. - : Springer Science and Business Media LLC. - 1359-4184 .- 1476-5578. ; 23:1, s. 133-142
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Tidskriftsartikel (refereegranskat)abstract
- The hypothesis that the S allele of the 5-HTTLPR serotonin transporter promoter region is associated with increased risk of depression, but only in individuals exposed to stressful situations, has generated much interest, research and controversy since first proposed in 2003. Multiple meta-analyses combining results from heterogeneous analyses have not settled the issue. To determine the magnitude of the interaction and the conditions under which it might be observed, we performed new analyses on 31 data sets containing 38 802 European ancestry subjects genotyped for 5-HTTLPR and assessed for depression and childhood maltreatment or other stressful life events, and meta-analysed the results. Analyses targeted two stressors (narrow, broad) and two depression outcomes (current, lifetime). All groups that published on this topic prior to the initiation of our study and met the assessment and sample size criteria were invited to participate. Additional groups, identified by consortium members or self-identified in response to our protocol (published prior to the start of analysis) with qualifying unpublished data, were also invited to participate. A uniform data analysis script implementing the protocol was executed by each of the consortium members. Our findings do not support the interaction hypothesis. We found no subgroups or variable definitions for which an interaction between stress and 5-HTTLPR genotype was statistically significant. In contrast, our findings for the main effects of life stressors (strong risk factor) and 5-HTTLPR genotype (no impact on risk) are strikingly consistent across our contributing studies, the original study reporting the interaction and subsequent meta-analyses. Our conclusion is that if an interaction exists in which the S allele of 5-HTTLPR increases risk of depression only in stressed individuals, then it is not broadly generalisable, but must be of modest effect size and only observable in limited situations.
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| 2. |
- Mutafela, Richard, et al.
(författare)
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Sustainable extraction of hazardous metals from crystal glass waste using biodegradable chelating agents
- 2022
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Ingår i: Journal of Material Cycles and Waste Management. - : Springer Nature. - 1438-4957 .- 1611-8227. ; 24:2, s. 692-701
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Tidskriftsartikel (refereegranskat)abstract
- Extraction of hazardous metals from dumped crystal glass waste was investigated for site decontamination and resource recovery. Mechanically activated glass waste was leached with biodegradable chelating agents of ethylenediamine-N,N′-disuccinic acid (EDDS) and nitrilotriacetic acid (NTA), where the concentration and reaction time were determined by using Box–Wilson experimental design. Hazardous metals of lead (Pb), arsenic (As), antimony (Sb) and cadmium (Cd) with concentrations higher than regulatory limits were extracted wherein the extraction yield was found to vary Pb > Sb > As > Cd. Extraction was influenced more by type and concentration of chelator rather than by reaction time. A maximum of 64% of Pb could be extracted by EDDS while 42% using NTA. It is found that increase of chelator concentrations from 0.05 M to 1 M did not show improved metal extraction and the extraction improved with reaction time until 13 h. This study provides sustainable alternative for treating hazardous glass waste by mechanical activation followed by extraction using biodegradable chelator, instead of acid leaching. Graphical abstract: [Figure not available: see fulltext.].
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| 3. |
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| 4. |
- Berna, Nathalie, et al.
(författare)
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Cosolvent-induced adsorption and desorption of serum proteins on an amphiphilic mercaptomethylene pyridine-derivatized agarose gel
- 1996
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Ingår i: Arch Biochem Biophys. - : Elsevier BV. - 0003-9861. ; 330:1, s. 188-192
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Tidskriftsartikel (refereegranskat)abstract
- We studied the effects of the following cosolvents on the adsorption and desorption of serum proteins from an amphiphilic mercaptomethylene pyridine-derivatized agarose gel: glucose, sucrose, polyethylene glycol (PEG), 2-methyl-2,4-pentanediol (MFD), sorbitol, pentaerythritol, glycerol, and Na2SO4. The water-structuring salt 0.4 M Na2SO4 was the most potent promoter of protein adsorption, followed by 5 M sorbitol and, to a lesser extent, 0.2 M PEG 1000 and 2.25 M MPD. The other cosolvents (4 M glucose, 1.5 M sucrose, 0.3 M pentaerythritol, and 7.6 M glycerol) were unable to promote protein adsorption to the gel. Attempts to modulate the salt-promotion effect of Na2SO4 with different cosolvents demonstrated the occurrence of synergistic effects for pentaerythritol, sorbitol, and glucose and antagonistic effects for the other cosolvents. Sorbitol and glycerol were found to be the most interesting co-solvents studied, as the first promoted protein adsorption, whereas the other disrupted protein interaction. As a consequence of these novel findings we propose sorbitol and glycerol, both well-known protein stabilizers, as possible alternatives to water-structuring salts during the adsorption phase and to deleterious organic solvents during the desorption phase on amphiphilic gels.
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| 5. |
- Buijs, J, et al.
(författare)
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SPR-MS in Functional Proteomics
- 2005
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Ingår i: Briefings in Functional Genomics and Proteomics. - : Oxford University Press (OUP). - 1473-9550 .- 1477-4062. ; 4:1, s. 39-47
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Tidskriftsartikel (refereegranskat)abstract
- The mapping of protein networks and the establishment of the functional relationships between expressed proteins and their effects on cellular processes represents a great challenge for functional or interaction proteomics. The combination of surface plasmon resonance (SPR)-based technology with mass spectrometry (MS) has created a unique analytical tool for functional proteomics investigations. Proteins are affinity purified, quantified and characterised in terms of their interactions, while the mass spectrometer identifies and structurally characterises the biomolecules. Recent developments have led to a closer integration of these key technologies, providing a combined approach which enables identification of proteins selected on the basis of their functional binding criteria. In addition to a historical overview of this field, some recent detailed examples of combined SPR-MS approaches will be reviewed in a number of key application areas, including ligand fishing, peptide sequence and post-translational modification analysis by SPR-MS/MS and enzyme inhibitor screening.
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| 6. |
- Christianty, F. M., et al.
(författare)
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The effects of Carica papaya L. juice on plasma aspartate transaminase/ alanine transaminase level and liver histopathology in paracetamol-induced mice
- 2020
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Ingår i: Annals of Tropical Medicine and Public Health. - : Wolters Kluwer. - 1755-6783 .- 0974-6005. ; 23:8, s. 1248-1252
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Tidskriftsartikel (refereegranskat)abstract
- Liver is one of the primary and largest metabolic organs in the body. The function of the liver can be disturbed and damaged by paracetamol and liver damage can be prevented with papaya (Carica papaya L.). The purpose of this study was to know the effects of C. papaya juice on plasma AST/ALT level and liver histopathology in paracetamol-induced mice. Amount 25 Balb-C mice (Mus musculus) were divided into five treatment groups, there were normal, negative control, papaya juice dose (200, 400, 600) mg kg-1 bw-1 groups. The treatment was carried out for 7 d and then all mice (without normal groups) was induced by paracetamol. On the 8th d, blood was collected and all mice were sacrificed. The materials used in this study include C. papaya juice, CMC Na, paracetamol, AST and ALT reagents, Hematoxylin-Eosin and used Experimental Laboratory methods. One Way ANOVA and Kruskal Wallis with a confidence level of 95 % and followed by a post hoc test (LSD) to determine differences between groups. Showed that pretreatment with papaya juice could prevent liver damage and inhibit the increase of plasma AST/ALT level and papaya juice dose 600 mg kg-1 bw-1 had significant effects on plasma AST/ALT level and liver histopathology in paracetamol-induced mice. Carica papaya L. juice can be used as a hepatoprotective agent.
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| 7. |
- Kurniawati, A., et al.
(författare)
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Analysis of increasing IFN-γ expression in mice's lung tissue infected with Mycobacterium tuberculosis by giving purple leaf methanol extract
- 2020
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Ingår i: Annals of Tropical Medicine and Public Health. - : Wolters Kluwer. - 1755-6783 .- 0974-6005. ; 23:3, s. 23-33
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Tidskriftsartikel (refereegranskat)abstract
- Context: Tuberculosis is an infectious disease that highly depends on the immune response. Purple leaves (Graptophyllum pictum (L) Griff) has an immune-modulatory activity. Aims: The purpose of this study was to analyze the effect of purple leaf methanol extract (EMDU) on the expression of IFN-γ in mice lung tissue infected with Mycobacterium tuberculosis. Materials and Methods: M. tuberculosis was infected in mice. The EMDU was given with dose (1.703, 3.406, 6.812) mg kg-1 BW-1 for 14 d after infection. The expression of IFN-γ protein (expression obtained from mice's lung tissue) was examined using immunohistochemical examination using IFN-γ anti monoclonal antibodies. Calculations performed on immunoreactive cells showed positive expression and reddish-brown appearance on the cytoplasm. Calculated as many as ten fields of view using a light microscope at 400 times magnification, then the mean value is taken. The mean value of the number of immunoreactive cells is included as data. Data analysis by one way ANOVA and Duncan test. Results: The treatment group showed that IFN-γ expression in mice tuberculosis was significantly increased (p < 0.05) of EMDU. Conclusions: Administration of EMDU increase of IFN-γ expression in mice tuberculosis.
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| 8. |
- Larseriksdotter, Helén, et al.
(författare)
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Optimizing the surface plasmon resonance/mass spectrometry interface for functional proteomics applications
- 2006
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Ingår i: Proteomics. - 1615-9853. ; 6:8, s. 2355-2364
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Tidskriftsartikel (refereegranskat)abstract
- A great challenge in functional or interaction proteomics is to map protein networks and establish a functional relationship between expressed proteins and their effects on cellular processes. These cellular processes can be studied by characterizing binding partners to a "bait" protein against a complex background of other molecules present in cells, tissues, or biological fluids. This so-called ligand fishing process can be performed by combining surface plasmon resonance biosensors with MS. This combination generates a unique and automated method to quantify and characterize biomolecular interactions, and identify the interaction partners. A general problem in chip-based affinity separation systems is the large surface-to-volume ratio of the fluidic system. Extreme care, therefore, is required to avoid nonspecific adsorption, resulting in losses of the target protein and carry-over during the affinity purification process, which may lead to unwanted signals in the final MS analysis and a reduction in sensitivity. In this study, carry-over of protein and low-molecular weight substances has been investigated systematically and cleaning strategies are presented. Furthermore, it is demonstrated that by the introduction of colloidal particles as a capturing and transporting agent, the recovery yield of the affinity-purified ligand could be improved nearly twofold.
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| 9. |
- Leithaeuser, Anna, et al.
(författare)
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Comparison of pyrochar, hydrochar and lignite as additive in anaerobic digestion and NH4+ adsorbent
- 2022
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Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 361
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Tidskriftsartikel (refereegranskat)abstract
- The impact of pyrochar, hydrochar and lignite addition on anaerobic digestion of food waste was investigated with and without ammonia inhibition under batch conditions. Furthermore, ammonium adsorption capacities of the chars were investigated. To determine anaerobic degradation of char, reference samples containing inoculum and char were analyzed, indicating a significant degradation of hydrochar. Depending on the evaluation method, the increase in methane yield due to hydrochar addition varied between no statistically significant difference and +14 %. No significant impact due to the addition of 5 g/l pyrochar and lignite on AD was found. NH4+ adsorption capacities showed a significantly higher net adsorption capacity of lignite (1.58 mgNH4+/gL), compared to pyrochar (0.63 mgNH4+/gPC). A negative NH4+ adsorption capacity (−0.51 mgNH4+/gHC) was found for hydrochar. A high H/C-ratio, O/C-ratio and cation exchange capacity of hydrochar and lignite indicate many functional groups and low chemical stability, enabling an increased interaction between NH4+ and char.
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| 10. |
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