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| 2. |
- Mamo, Gashaw, et al.
(författare)
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Preface
- 2020
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Ingår i: Alkaliphiles in Biotechnology. - 0724-6145. ; 172
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 3. |
- Bergdahl, Gizem Ertürk, et al.
(författare)
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Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
- 2019
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Ingår i: Applied Biochemistry and Biotechnology. - : Springer Science and Business Media LLC. - 0273-2289 .- 1559-0291. ; 189, s. 374-383
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Tidskriftsartikel (refereegranskat)abstract
- A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.
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| 4. |
- Idil, Neslihan, et al.
(författare)
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Imprinting of microorganisms for biosensor applications
- 2017
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Ingår i: Sensors. - : MDPI AG. - 1424-8220. ; 17:4
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Forskningsöversikt (refereegranskat)abstract
- There is a growing need for selective recognition of microorganisms in complex samples due to the rapidly emerging importance of detecting them in various matrices. Most of the conventional methods used to identify microorganisms are time-consuming, laborious and expensive. In recent years, many efforts have been put forth to develop alternative methods for the detection of microorganisms. These methods include use of various components such as silica nanoparticles, microfluidics, liquid crystals, carbon nanotubes which could be integrated with sensor technology in order to detect microorganisms. In many of these publications antibodies were used as recognition elements by means of specific interactions between the target cell and the binding site of the antibody for the purpose of cell recognition and detection. Even though natural antibodies have high selectivity and sensitivity, they have limited stability and tend to denature in conditions outside the physiological range. Among different approaches, biomimetic materials having superior properties have been used in creating artificial systems. Molecular imprinting is a well suited technique serving the purpose to develop highly selective sensing devices. Molecularly imprinted polymers defined as artificial recognition elements are of growing interest for applications in several sectors of life science involving the investigations on detecting molecules of specific interest. These polymers have attractive properties such as high bio-recognition capability, mechanical and chemical stability, easy preparation and low cost which make them superior over natural recognition reagents. This review summarizes the recent advances in the detection and quantification of microorganisms by emphasizing the molecular imprinting technology and its applications in the development of sensor strategies.
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| 5. |
- Kumar, Ashok, et al.
(författare)
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Integrated bioprocess for the production and isolation of urokinase from animal cell culture using supermacroporous cryogel matrices
- 2006
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Ingår i: Biotechnology and Bioengineering. - Hoboken, NJ : Wiley. - 1097-0290 .- 0006-3592. ; 93:4, s. 636-646
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Tidskriftsartikel (refereegranskat)abstract
- An integrated cell cultivation and protein product separation process was developed using a new type of supermacroporous polyacrylamide gel, called cryogel (pAAm-cryogel) support matrix. Human fibrosarcoma HT1080 and human colon cancer HCT116 cell lines were used to secrete urokinase (an enzyme of immense therapeutic utility) into the culture medium. The secreted protein was isolated from the circulating medium using a chromatographic capture column. A pAAm cryogel support with covalently coupled gelatin (gelatin-pAAm cryogel) was used for the cultivation of anchorage dependent cells in the continuous cell culture mode in 5% carbon dioxide atmosphere. The cells were attached to the matrix within 4-6 h of inoculation and grew as a tissue sheet inside the cryogel matrix. Continuous urokinase secretion into the circulating medium was monitored as a parameter of growth and viability of cells inside the bioreactor. No morphological changes were observed in the cells eluted from the gelatin-cryogel support and re-cultured in T-flask. The gelatin-pAAm cryogel bioreactor was further connected to a pAAm cryogel column carrying Cu(II)-iminodiacetic acid (Cu(II)-IDA)-ligands (Cu(II)-IDA-pAAm cryogel), which had been optimized for the capture of urokinase from the conditioned medium of the cell lines. Thus an automated system was built, which integrated the features of a hollow fiber reactor with a chromatographic protein separation system. The urokinase was continuously captured by the Cu(II)-IDA-pAAm cryogel column and periodically recovered through elution cycles. The urokinase activity increased from 250 PU/mg in the culture fluid to 2,310 PU/mg after recovery from the capture column which gave about ninefold purification of the enzyme. Increased productivity was achieved by operating integrated bioreactor system continuously for 32 days under product inhibition free conditions during which no back-pressure or culture contamination was observed. A total 152,600 Plough units of urokinase activity was recovered from 500 mL culture medium using 38 capture columns over a period of 32 days. (c) 2006 Wiley Periodicals, Inc.
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| 6. |
- Perçin, Isık, et al.
(författare)
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Microcontact imprinted plasmonic nanosensors : Powerful tools in the detection of salmonella paratyphi
- 2017
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Ingår i: Sensors. - : MDPI AG. - 1424-8220. ; 17:6
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Tidskriftsartikel (refereegranskat)abstract
- Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 × 106-15 × 106 CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 × 106 CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.
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| 7. |
- Liu, Jing, et al.
(författare)
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A volumetric meter for monitoring of low gas flow rate from laboratory-scale biogas reactors
- 2004
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Ingår i: Sensors and Actuators B: Chemical. - : Elsevier BV. - 0925-4005. ; 97:2-3, s. 369-372
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Tidskriftsartikel (refereegranskat)abstract
- A reliable and inexpensive device for measuring gas with low flow rates is described. The device works based on the principle of liquid displacement and is able to measure flows from 1 to 950 ml(.)h(-1). It also has improved counting logic and data processing capacity to avoid miscounting and provide a friendly interface for data display. (C) 2003 Elsevier B.V. All rights reserved.
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| 8. |
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| 9. |
- Liu, Jing, et al.
(författare)
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Control of an anaerobic reactor towards maximum biogas production
- 2004
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Ingår i: Water Science and Technology. - 0273-1223. ; 50:11, s. 189-198
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Tidskriftsartikel (refereegranskat)abstract
- A new control strategy is introduced for operating anaerobic digestion processes efficiently at high load. The control system includes a cascade controller embedded into a rule-based supervisory system based on extremum-seeking control. The control system measures pH and biogas production rate and varies the organic load by manipulating the influent flow. Good control performances were achieved during the start-up and steady-state running operations and during rejection of disturbances. The control system can run the process under a high load condition and efficiently reject disturbances without explicit measurement of the influent characteristics.
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| 10. |
- Liu, Jing, et al.
(författare)
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Extremum-seeking with variable gain control for intensifying biogas production in anaerobic fermentation
- 2006
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Ingår i: Water Science and Technology. - : IWA Publishing. - 0273-1223 .- 1996-9732. ; 53:4-5, s. 35-44
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Tidskriftsartikel (refereegranskat)abstract
- A state-dependent variable-gain control system is implemented to follow the characteristics of a laboratory-scale up-flow anaerobic fixed-bed reactor dynamically. The transition from one state to another is determined on an hourly basis, depending on difference between the setpoint of the reactor pH and its true value. Considerable improvement of the process stability - reduction of oscillation in both the reactor pH and biogas production rate during high-rate operation, has been achieved, although the control structure is simple and intuitive.
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