| 1. |
|
|
| 2. |
|
|
| 3. |
- Fäldt, R., et al.
(författare)
-
Demonstration of antibody‐associated cellular cytotoxicity in patients with acute myelogenous leukemia before and after chemotherapy
- 1979
-
Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 24:1, s. 17-26
-
Tidskriftsartikel (refereegranskat)abstract
- This study demonstrates that a cytotoxic serum reactivity not requiring the presence of complement appears in the sera of patients with acute myelogenous leukemia. The reaction is detected upon short‐term incubation of sera in vitro with autochthonous mononuclear white blood cells from peripheral venous blood of patients at the acute stage of the disease. This reactivity was demonstrated in 18/18 patients. Generally, the cytotoxicity was low in patients at the acute stage of the disease, but increased after chemotherapy and reached the highest level at the onset of clinical remission or just before. No cytotoxicity could be demonstrated against autochthonous remission white blood cells. The serum activity could be absorbed and eluted from protein A‐Sepharose CL‐4B and was recovered in the 7S‐fraction of the sera after gel filtration on Sephadex G‐200 and ion exchange chromatography. This indicates that the demonstrated cytotoxicity is due to immunoglobulins of IgG‐class. It is believed that Fc‐receptor‐bearing cells present in the target cell preparations function as effector cells. The reaction is designated antibody‐associated cellular cytotoxicity (AACC).
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
- Olsson, H., et al.
(författare)
-
Relief of Pruritus as an Early Sign of Spinal Cord Compression in Hodgkin's Disease
- 1979
-
Ingår i: Journal of Internal Medicine. - : Wiley. - 0001-6101. ; 206:1-6, s. 319-320
-
Tidskriftsartikel (refereegranskat)abstract
- ABSTRACT In a patient with advanced Hodgkin's disease (HD) associated with generalized pruritus, an unexpected relief of itching was found to be an early sign of spinal cord compression. Following irradiation of an extradural mass at the Th II level, itching recurred. Although the mechanisms bringing about itching in HD are unknown, the relief and recurrence of the symptom in our patient are in line with a peripheral origin of pruritus in the disease. Spontaneous relief of pruritus in HD despite other signs of active disease should prompt a neurological examination, since early recognition and treatment of spinal cord compression in lymphoma are important to avoid residual neurological disability. 1979 Association for the Publication of the Journal of Internal Medicine
|
|
| 7. |
- Troye-Blomberg, Marita
(författare)
-
In vitro studies of natural and tumor-associated immunity in patients with transitional cell carcinoma of the urinary bladder
- 1979
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Peripheral blood lymphocytes (PBL) from patients with transitional cell carcinoma of the urinary bladder (TCC-bladder) were studied using a SlCr-release assay for their in vitro cytotoxicity against a panel of allogeneic tissue culture cell lines of TCC-bladder and other origins. Control PBL were obtained from four different groups of donors. Individual donor's lymphocytes frequently displayed cytotoxicity to any one of the target cells. However, PBL from each of the three TCC-bladder groups had strongly elevated mean cytotoxicity to the TCC-bladder targets as compared to that to the control targets. The control groups did not show this. The mean cytotoxicity of PBL from the TCC bladder groups to the bladder tumor targets was significantly higher than that of the control groups. The results suggest the existence of a disease related reactivity in TCC-bladder superimposed on background of natural cytotoxicity. Effector cell studies in allogeneic lymphocyte/tumor target combinations revealed that the cytotoxic effector cells within all donor groups were indistinguishable from the lymphocytes responsible for antibody-dependent cellular cytotoxicity (ADCC, K-cells). A possible involvement of antibodies in the cytotoxic reactions was studied by adding Fab-fragments of anti-human immunoglobulin antibodies (Fab-alg) to the PBL/target cell mixtures. These reagents inhibited cytotoxicity, although inhibition was usually incomplete. The results suggest the involvement of both antibody dependent and antibody independent mechanisms in the cell-mediated cytotoxicity (CMC) against allogeneic target cells. Investigations of effector cell mechanisms in autologous PBL/tumor target combinations revealed the presence of other mechanisms than seen in most allogeneic combinations. Thus, the effector cells were distinct from K-cells and the reactions were not inhibited by the presence of Fab-alg. The results suggest that these autologous reactions may be mediated by antibody independent cytolytic T-lymphocytes (CTL) and may require HLA-compatibility between effector cells and target cells. However, at the present stage, alternative explanations must also be considered. In the search for humoral antibodies to tumor cells in serum of TCC-bladder patients and controls an ADCC assay was used. To avoid inhibition by blocking factors present in whole serum, IgG-fractions were prepared. ADCC inducing antibodies were found in IgG-fractions from both TCC-bladder patients and controls. However, when tested against one cell line of TCC-bladder origin and one control cell line, the IgG-fractions from TCC-bladder patients were on the average more cytotoxic to the TCC-bladder target than to the control. This was not seen with the IgG-fractions of the controls. This suggests that TCC-bladder patients develop a humoral response related to their disease. However, the nature of the antigen(s), responsible for these reactions remains to be established.
|
|
