| 41. |
- KRISTOFFERSSON, ULF, et al.
(author)
-
CYTOGENETIC STUDIES IN HODGKIN'S DISEASE
- 1987
-
In: Acta Pathologica Microbiologica Scandinavica. Section A. Pathology. - 0108-0164. ; 95 A:1-6, s. 289-295
-
Journal article (peer-reviewed)abstract
- Cytogenetic analysis was attempted in 20 patients with Hodgkin's disease. No mitoses were found in 2 cases, normal metaphases in 7, and normal metaphases with nonclonal aberrations in 7. Of the 4 cases with clonal aberrations, one had +16 as the sole change, whereas the remaining tumors had multiple numerical and structural changes.
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| 42. |
- KRISTOFFERSSON, ULF, et al.
(author)
-
Cytogenetic studies in non‐Hodgkin lymphomas‐Results from fineneedle aspiration samples
- 1985
-
In: Hereditas. - : Springer Science and Business Media LLC. - 0018-0661. ; 103:1, s. 63-76
-
Journal article (peer-reviewed)abstract
- 118 samples obtained by means of fine‐needle aspiration from 86 cases of non‐Hodgkin, non‐Burkitt lymphomas have been investigated cytogenetically with chromosome banding technique. If>5 times 106 cells were obtained at aspiration the success rate with the technique used was almost 70%, while if the number of cells was lower it was only about 15%. Technical aspects, and detailed cytogenetic findings in 48 successful cases, are reported. There was no difference in success rate due to tumor stage, or sampling at diagnosis or in relapse. It is concluded that the fine‐needle aspiration technique has certain advantages in cancer cytogenetics; it is easy to handle, requires no surgical intervention, causes little distress to the patient, and the success rate is acceptable.
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| 43. |
- KRISTOFFERSSON, ULF, et al.
(author)
-
No abnormal C‐band polymorphism in lung cancer patients
- 1989
-
In: Hereditas. - : Springer Science and Business Media LLC. - 0018-0661. ; 110:3, s. 201-202
-
Journal article (peer-reviewed)abstract
- The C‐band heterochromatin polymorphism of chromosomes 1, 9, and 16 was studied in lymphocytes from 52 lung cancer patients and 183 control persons. No significant differences between the controls and patients were found regarding heterochromatin block size, the frequency of partial and total inversions, or the symmetry/asymmetry pattern.
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| 44. |
- Kristoffersson, Ulf, et al.
(author)
-
Prognostic implication of cytogenetic findings in 106 patients with non-Hodgkin lymphoma
- 1987
-
In: Cancer Genetics and Cytogenetics. - : Elsevier BV. - 0165-4608. ; 25:1, s. 55-64
-
Journal article (peer-reviewed)abstract
- The cytogenetic findings in samples from 106 patients with non-Hodgkin lymphomas (NHL), histopathologically classified according to the Kiel classification, have been correlated with survival time. Clonal chromosomal abnormalities were found in 60 patients, and only normal karyotypes in ten. The chromosome analysis of the remaining samples failed. The failures did not differ in survival compared with the cytogenetically successful cases, indicating that this group is not a prognostic entity within NHL. The cytogenetic findings were classified in six ways in order to evaluate the prognostic value of the cytogenetic pattern. Multivariate analysis demonstrated that presence of clonal chromosome abnormalities and the number of aberrations both were important prognostic factors independent of histopathology, whereas, the modal chromosome number, presence of translocations, or unidentified marker chromosomes were not. Some characteristic chromosome abnormalities were correlated with survival time: Patients with a 1p+ marker or +7 had a significantly shorter survival time than patients with normal karyotypes only (NN). Patients with +3, +12, 6q-, i(17q), and t(14;18)(q32;q21) did not differ significantly from the NN group.
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| 45. |
- KRISTOFFERSSON, ULF, et al.
(author)
-
RELATIONSHIP BETWEEN CYTOGENETIC FINDINGS AND HISTOPATHOLOGY IN NON‐HODGKIN LYMPHOMA
- 1987
-
In: Acta Pathologica Microbiologica Scandinavica. Section A. Pathology. - 0108-0164. ; 95 A:1-6, s. 1-5
-
Journal article (peer-reviewed)abstract
- The cytogenetic findings in 70 patients with non‐Hodgkin lymphoma have been correlated with tumor histopathology according to the Kiel classification. Certain chromosome aberrations displayed a nonrandom association with the grade of malignancy: 4 lymphomas out of 6 with 1p+, 5 out of 7 with del(6)(q15), 7 out of 11 with 14q+, and 5 out of 8 with +18 belonged to the high grade malignancy group, whereas 9 lymphomas out of 10 with t(14;18) were low grade malignant. Two aberration types were closely associated with specific histopathologic subtypes: t(14; 18) occurred in 7 cases out of 10 in centroblastic/ centrocytic (cb/cc) follicular lymphomas, and 5 cases out of 6 with i(17q) were cb or cb/cc. Although less striking, there was a tendency for del(6)(q15) to occur in cb or cb/cc lymphomas (4 cases out of 7), in contrast to only 1 case out of 5 with the more distal deletion del(6)(q21).
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| 46. |
- Kristoffersson, Ulf, et al.
(author)
-
Trisomy 5 and t(5;14)(q11;q32) as the sole abnormalities in two different clones from a centroblastic non-Hodgkin's lymphoma
- 1988
-
In: Cancer Genetics and Cytogenetics. - : Elsevier BV. - 0165-4608. ; 36:2, s. 173-176
-
Journal article (peer-reviewed)abstract
- A 62-year-old previously healthy woman presented with a centroblastic non-Hodgkin's lymphoma in the thyroid. Chromosome analysis revealed two unrelated clones, 47,XX,+5 and 46,XX,-14,+der(14)t(5;14)(q11;q32). The two clones may reflect a polyclonal origin, or they may be the descendants of the same neoplastically rearranged cell. In the latter case, the clonal aberrations are either secondary to an event detectable only at the molecular level, or one of them is a primary cytogenetic event while the other arose through clonal evolution with loss of the primary aberration. The best candidate for the primary change would be trisomy 5. Trisomy 5 has previously been associated with lymphomas with diffuse, large, noncleaved morphology, a group within the Working Formulation largely equivalent to centroblastic lymphomas in the Kiel classification. Our findings thus support the notion that trisomy 5 may be associated with centroblastic/diffuse, large, noncleaved lymphomas.
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| 47. |
- Linden, Margareta, et al.
(author)
-
Cell cycle phase-dependent induction of ornithine decarboxylase-antizyme.
- 1985
-
In: Journal of Cellular Physiology. - : Wiley. - 1097-4652 .- 0021-9541. ; 125:2, s. 273-276
-
Journal article (peer-reviewed)abstract
- The activites of ornithine decarboxylase (ODC) and ODC inhibitory protein (ODC-antizyme) were studied in Ehrlich ascites tumor cells, separated according to their position in the cell cycle by centrifugal elutriation. Release and/or synthesis of ODC-antizyme was induced by putrescine treatment. Each mouse received an intraperitoneal injection of 25 moles of putrescine at 0, 1, 2, and 3 hr after tumor transplantation. Tumor cells obtained from putrescine-treated and control mice at 4 hr after transplantation were separated into fractions representing all phases of the cell cycle. The cell cycle distribution of the tumor cells in each fraction was determined by flow cytometry. In control tumor cells the ODC activity exhibited two maxima; inlate-G1/early-S and in late-S/G2. A marked decrease in ODC activity was observed in mid-S phase. This decrease coincided with maximum ODC-antizyme activity (revealed by putrescine treatment), suggesting that ODC-antizyme is involved in the regulation of ODC activity during the cell cycle.
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| 48. |
- Lindhqvist, Violetta, et al.
(author)
-
Comparison of the mitogenic activities of streptococcal protein-G and staphylococcal protein-A on human mononuclear cells
- 1989
-
In: Immunological Investigations. - : Informa UK Limited. - 0882-0139 .- 1532-4311. ; 18:7, s. 919-930
-
Journal article (peer-reviewed)abstract
- In the present work we report data from experiments comparing the proliferative stimuli demonstrated by Streptococcal Protein-G and Staphylococcal Protein A on human peripheral blood mononuclear cells. Protein-G and Protein-A are presented to the cells in solution as well as linked to plastic or Sepharose beads, or incorporated within the cell wall of whole bacteria. The cellular response is measured by incorporation of 3H-thymidine in 72 hr cultures. The soluble and the immobilized forms of Protein-A, but not those of Protein-G, displayed high mitogenicity. Possible explanations for the absence of Protein-G induced mitogenicity are discussed.
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| 49. |
- Mattsson, J, et al.
(author)
-
Intratumoral distribution of microspheres.
- 1986
-
In: Anticancer research. - 0250-7005. ; 6:4, s. 563-6
-
Journal article (peer-reviewed)abstract
- Fifteen mu carbonized microspheres injected in two transplantable rat tumors, a hepatoma transplanted to the dorsum of one hindpaw and an adenocarcinoma transplanted into the liver, were found to be unevenly distributed and often in aggregates that seemed to fill intratumoral vessels. This finding can partly explain the increased vascular resistance in tumors after microsphere injection and makes the technique with repeated injections of microspheres disputable.
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| 50. |
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