| 1. |
- Belfrage, Per, et al.
(författare)
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Dispersion of viable pig liver cells with collagenase
- 1975
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Ingår i: Life Sciences. - : Elsevier BV. - 1879-0631 .- 0024-3205. ; 17:8, s. 1219-1225
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Tidskriftsartikel (refereegranskat)abstract
- Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.
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| 2. |
- Belfrage, Per, et al.
(författare)
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Alterations of lipid metabolism in healthy volunteers during long-term ethanol intake
- 1977
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Ingår i: European Journal of Clinical Investigation. - : Wiley. - 0014-2972 .- 1365-2362. ; 7:2, s. 127-131
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Tidskriftsartikel (refereegranskat)abstract
- Nine young, healthy male volunteers were given ethanol (75 g/day) for 5 weeks. The ethanol was divided into five daily doses and taken so that blood ethanol levels never exceeded 0.04% (w/v). During the latter part of the ethanol intake period, there was a significant, transient increase of plasma triglyceride (TG) concentrations followed by reduction to normal levels. A three-fold increase of lipoprotein lipase activity (LLA) occurred in biopsy specimens of adipose tissue. An increase of alpha-lipoprotein concentrations, which correlated significantly with the decrease in plasma TG levels and the increase in adipose LLA, was also observed during the ethanol intake period. No changes were observed in plasma cholesterol and beta-lipoprotein levels. A transient, three-fold increase of TG concentrations occurred in liver biopsy specimens. Ultrastructural and cytochemical examinations of the biopsy specimens showed hyperplasia of the smooth endoplasmic reticulum, and increased canallicular activity of gamma-glutamyl transferase (gamma-GT) activity in most subjects towards the end of and after the ethanol intake period. Serum gamma-GT levels also increased significantly.
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| 3. |
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| 4. |
- Nelson, K, et al.
(författare)
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Separation of rat leukocytes by countercurrent distribution in aqueous two-phase systems. II. Subpopulations which mediate selective and nonselective lysis of normal and colon carcinoma target cells in vitro
- 1978
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Ingår i: Cellular Immunology. - : Elsevier BV. - 0008-8749. ; 37:2, s. 422-431
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Tidskriftsartikel (refereegranskat)abstract
- Spleen cells from WF rats immunized to allogeneic lymphoid cells or to syngeneic colon carcinomas and from unimmunized controls were separated by countercurrent distribution in aqueous two-phase systems. The cells were assayed for cytotoxicity to allogeneic fibroblasts or syngeneic colon carcinoma cells and to syngeneic fibroblasts in a 24-hr 51Cr-release assay. Cells from immunized rats which selectively lysed the specific target cells were repeatedly found in one area of the distribution separate from the majority of cells, which nonselectively lysed syngeneic fibroblasts as well. A similar subpopulation which nonselectively lysed all target cells assayed was recovered from the spleens of unimmunized rats. The cells were also assayed for the ability to lyse antibody-coated thymocytes in a 4-hr 51Cr-release assay. The peak of K cells was found to overlap partially that of cells with nonselective cytotoxicity.
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| 5. |
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| 6. |
- Fäldt, R., et al.
(författare)
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Demonstration of antibody‐associated cellular cytotoxicity in patients with acute myelogenous leukemia before and after chemotherapy
- 1979
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 24:1, s. 17-26
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Tidskriftsartikel (refereegranskat)abstract
- This study demonstrates that a cytotoxic serum reactivity not requiring the presence of complement appears in the sera of patients with acute myelogenous leukemia. The reaction is detected upon short‐term incubation of sera in vitro with autochthonous mononuclear white blood cells from peripheral venous blood of patients at the acute stage of the disease. This reactivity was demonstrated in 18/18 patients. Generally, the cytotoxicity was low in patients at the acute stage of the disease, but increased after chemotherapy and reached the highest level at the onset of clinical remission or just before. No cytotoxicity could be demonstrated against autochthonous remission white blood cells. The serum activity could be absorbed and eluted from protein A‐Sepharose CL‐4B and was recovered in the 7S‐fraction of the sera after gel filtration on Sephadex G‐200 and ion exchange chromatography. This indicates that the demonstrated cytotoxicity is due to immunoglobulins of IgG‐class. It is believed that Fc‐receptor‐bearing cells present in the target cell preparations function as effector cells. The reaction is designated antibody‐associated cellular cytotoxicity (AACC).
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| 7. |
- Fäldt, R., et al.
(författare)
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Tumor‐associated humoral cytotoxicity in patients with acute myelogenous leukemia before and after chemotherapy
- 1977
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 20:6, s. 824-833
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Tidskriftsartikel (refereegranskat)abstract
- Sera of eight unselected adult patients with acute myelogenous leukemia obtained before and after chemotherapy were repeatedly tested for specific complement‐dependent cytotoxicity against autochthonous peripheral white blood cells from the acute leukemia stage and from the remission stage, respectively. Complement‐dependent cytotoxicity against white blood cells from the acute leukemia stage was demonstrated in all of the eight patients, while none of three patients' sera were reactive against white blood cells from the remission stage tested in parallel. The cytotoxicity was increased after chemotherapy, also in those patients in whom remission was not achieved.
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| 8. |
- Johansson, C, et al.
(författare)
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Interaction by cholestyramine on the uptake of hydrocortisone in the gastrointestinal tract.
- 1978
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Ingår i: Acta medica Scandinavica. - 0001-6101. ; 204:6, s. 509-12
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Tidskriftsartikel (refereegranskat)abstract
- An absolute reduction of the plasma cortisol levels and a delay of the peak concentrations were recorded in 10 healthy subjects, when a bile-sequestering anionic exchange resin, cholestyramine, was given prior to a single oral hydrocortisone dose, indicating that the resin interferes with the uptake of a neutral sterol in the human gastrointestinal tract. The possibility of a direct binding of drug to resin is supported by the affinity of hydrocortisone to cholestyramine in vitro, which was uninfluenced by the presence of sodium taurocholate. Cholestyramine significantly delayed the gastric emptying of a glucose solution, indicating that the resin not only decreases but also delays hydrocortisone absorption. Careful supervision is recommended when treatment with cholestyramine is given concomitant to neutral sterol drugs.
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| 9. |
- Jonsson, Nils, et al.
(författare)
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Studies on adenovirus type 9‐induced mammary fibroadenomas in rats and their malignant transformation
- 1977
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Ingår i: Cancer. - 0008-543X. ; 39:6, s. 2513-2519
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Tidskriftsartikel (refereegranskat)abstract
- After inoculating newborn W/Fu rats with adenovirus type 9,27 of 27 females developed mammary fibroadenomas with a latency period of 14–25 weeks. No tumors were observed after inoculation with adenovirus type 5 or in males with the type 9 inoculation. After persistence of the tumors for 3–14 months, malignant transformation of the stroma resulted in different types of sarcoma in three rats: fibrosarcoma, round‐cell liposarcoma, osteosarcoma and malignant mes‐enchymoma. In another animal the stroma of a fibroadenoma was highly cellular, suggesting a transition into fibrosarcoma. Malignant transformation of the epithelial component was not observed. Tumor cells contained adenovirus type 9‐specific T‐antigen, and rats with transplanted tumors were immunized to T‐antigen. Mammary fibroadenomas without signs of malignant transformation developed in eight of nine female rats inoculated with adenovirus type 9 at an adult age. Neonatal thymectomy and total body x‐irradiation neither significantly shortened the induction time of adenovirus 9‐induced fibroadenomas nor increased the frequency of malignant transformation in females. One lipoma and one highly differentiated liposarcoma, however, appeared in two male rats. The results provide an example of the progression of a virus‐induced benign tumor into a malignant neoplasm.
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| 10. |
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