| 1. |
- Belfrage, Per, et al.
(författare)
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Dispersion of viable pig liver cells with collagenase
- 1975
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Ingår i: Life Sciences. - : Elsevier BV. - 1879-0631 .- 0024-3205. ; 17:8, s. 1219-1225
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Tidskriftsartikel (refereegranskat)abstract
- Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.
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| 2. |
- Belfrage, Per, et al.
(författare)
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Alterations of lipid metabolism in healthy volunteers during long-term ethanol intake
- 1977
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Ingår i: European Journal of Clinical Investigation. - : Wiley. - 0014-2972 .- 1365-2362. ; 7:2, s. 127-131
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Tidskriftsartikel (refereegranskat)abstract
- Nine young, healthy male volunteers were given ethanol (75 g/day) for 5 weeks. The ethanol was divided into five daily doses and taken so that blood ethanol levels never exceeded 0.04% (w/v). During the latter part of the ethanol intake period, there was a significant, transient increase of plasma triglyceride (TG) concentrations followed by reduction to normal levels. A three-fold increase of lipoprotein lipase activity (LLA) occurred in biopsy specimens of adipose tissue. An increase of alpha-lipoprotein concentrations, which correlated significantly with the decrease in plasma TG levels and the increase in adipose LLA, was also observed during the ethanol intake period. No changes were observed in plasma cholesterol and beta-lipoprotein levels. A transient, three-fold increase of TG concentrations occurred in liver biopsy specimens. Ultrastructural and cytochemical examinations of the biopsy specimens showed hyperplasia of the smooth endoplasmic reticulum, and increased canallicular activity of gamma-glutamyl transferase (gamma-GT) activity in most subjects towards the end of and after the ethanol intake period. Serum gamma-GT levels also increased significantly.
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| 3. |
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| 4. |
- Nelson, K, et al.
(författare)
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Separation of rat leukocytes by countercurrent distribution in aqueous two-phase systems. II. Subpopulations which mediate selective and nonselective lysis of normal and colon carcinoma target cells in vitro
- 1978
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Ingår i: Cellular Immunology. - : Elsevier BV. - 0008-8749. ; 37:2, s. 422-431
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Tidskriftsartikel (refereegranskat)abstract
- Spleen cells from WF rats immunized to allogeneic lymphoid cells or to syngeneic colon carcinomas and from unimmunized controls were separated by countercurrent distribution in aqueous two-phase systems. The cells were assayed for cytotoxicity to allogeneic fibroblasts or syngeneic colon carcinoma cells and to syngeneic fibroblasts in a 24-hr 51Cr-release assay. Cells from immunized rats which selectively lysed the specific target cells were repeatedly found in one area of the distribution separate from the majority of cells, which nonselectively lysed syngeneic fibroblasts as well. A similar subpopulation which nonselectively lysed all target cells assayed was recovered from the spleens of unimmunized rats. The cells were also assayed for the ability to lyse antibody-coated thymocytes in a 4-hr 51Cr-release assay. The peak of K cells was found to overlap partially that of cells with nonselective cytotoxicity.
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| 5. |
- Ankerst, J., et al.
(författare)
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Cross-reacting Tumor-associated Antigen(s) of Adenovirus Type 9-induced Fibroadenomas and a Chemically Induced Mammary Carcinoma in Rats
- 1974
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Ingår i: Cancer Research. - 0008-5472. ; 34:8, s. 1794-1800
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Tidskriftsartikel (refereegranskat)abstract
- Cross-reactivities among tumor-associated antigens of rat mammary fibroadenomas and among these fribroadenomas and a rat mammary carcinoma were investigated by in vitro techniques. Lymphocytes from five female W/Fu rats bearing mammary fibroadenomas were found to share a common reactivity against fibroadenoma target cells, as demonstrated by lymphocyte cytotoxicity tests on iododeoxyuridine-l25I-labeled target cells and by microcytotoxicity tests. These lymphocytes were also cytotoxic to target cells derived from a rat mammary carcinoma induced by 3,2'-dimethyl-4-aminobiphenyl. Inversely, lymphocytes from a female W/Fu rat bearing this 3,2'-dimethyl-4-aminobiphenyl-induced mammary carcinoma were cytotoxic to the carcinoma and the fibroadenoma target cells. Neither the immune lymphocytes from fibroadenoma-bearing rats nor those from the rats with the mammary carcinoma were cytotoxic to polyoma virus-induced sarcoma cells or to normal rat breast cells. Neither immune lymphocytes from rats with polyoma tumors nor those bearing chemically induced colon carcinomas demonstrated cellular immunity against either mammary fibroadenoma or mammary carcinoma target cells. Sera from each of the fibroadenoma-bearing rats inhibited the cytotoxic effect of lymphocytes from rats bearing fibroadenoma or carcinoma against both mammary fibroadenoma and carcinoma target cells. Sera from the rat with the 3,2'-dimethyl-4-aminobiphenyl-induced mammary carcinoma inhibited the activity of its own lymphocytes on either fibroadenoma or carcinoma target cells. They also blocked the cytotoxicity of fibroadenoma-immune lymphocytes to fibroadenoma or to carcinoma target cells. The blocking sera of mammary fibroadenomatous and carcinomatous rats did not inhibit the cytotoxic effect of lymphocytes from polyoma sarcoma-bearing rats against polyoma tumor cells. Additionally, sera from rats with either polyoma virus-induced tumors or colon carcinomas, which blocked in their respective systems, did not inhibit the in vitro activity of mammary fibroadenoma or carcinomaimmune lymphocytes on mammary fibroadenoma or cacinoma target cells. These results indicate a common tumor-associated antigenicity among the mammary fibroadenomas and a shared (tissue type-specific?) antigen between the mammary fibroadenomas and the chemically induced mammary carcinoma.
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| 6. |
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| 7. |
- Ankerst, Jaro
(författare)
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Demonstration And Identification Of Cytotoxic Antibodies And Antibodies Blocking The Cell-Mediated Antitumor Immunity Against Adenovirus Type 12 Induced Tumors
- 1971
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Ingår i: Cancer Research. - 0008-5472. ; 31:7, s. 997-1003
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Tidskriftsartikel (refereegranskat)abstract
- Hyperimmune mouse antisera against syngeneic adenovirus type 12 induced tumors and their 7 S and 19 S fractions of immunoglobulins obtained after combined diethylaminoethyl cellulose and Sephadex G-200 separation were tested against a rat adenovirus type 12 induced tumor by 51 Cr release technique. The same sera and their fractions were investigated with the colony inhibition technique for ability to abrogate the inhibitory effect of mouse lymph node cells specifically immunized against syngeneic adenovirus type 12 induced tumors on rat adenovirus 12 target tumor cells. It was found that (a) hyperimmune antisera from mice which had received many immunization doses were cytotoxic to rat adenovirus 12 induced tumor cells at 37° in the presence of complement; (b) the same hyperimmune antisera abrogated specifically the inhibitory effect of specifically immune mouse lymph node cells on rat adenovirus 12 tumor cells; (c) the cytotoxic activity of the hyperimmune antisera at 37° in the presence of complement was found in 19 S fractions of γ-globulins; and (d) the serum factors causing abrogation of immune lymph node cells in vitro were found in 7 S fractions of immunoglobulins.
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| 8. |
- Ankerst, Jaro, et al.
(författare)
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Demonstration of two group‐specific TSTAs in adenovirus‐induced tumors
- 1970
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 6:1, s. 84-92
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Tidskriftsartikel (refereegranskat)abstract
- Infection of mice and rats with any one of five tested human adenovirus types belonging to groups A and B (types 3, 7, 12, 18 and 31) induced an immunity to the TSTAs of type 12 tumors as detected by the colony inhibition (CI) test. No immunity was found against an adenovirus type 1 tumor. Two adenovirus types belonging to group C (types 1 and 5) were similarly tested and found to induce a clear‐cut immunity to the adenovirus type 1 tumor but not to tumors induced by adenovirus of groups A and B. The only tested virus type of group D (type 4) did not induce any clear‐cut immunity to either adenovirus 12 tumors or the adenovirus 1 tumor. Immunization of rats with rat adenovirus 12 tumor cells induced a cellular immunity to adenovirus 12 mouse tumor cells but not to a mouse polyoma tumor. Adenovirus 1 rat tumor cells induced no such immunity. Immunization of rats with syngenic adenovirus 12 tumor cells induced a cellular immunity to adenovirus 12 rat and mouse tumors and an adenovirus 7 hamster tumor, but not to an adenovirus 1 rat tumor or BHK‐C13 control hamster cells. The result of a tumor prevention experiment is consistent with the existence of a common TSTA induced by types 7 and 12 and a different TSTA induced by type 5 virus. It is concluded that the highly oncogenic group A and the weakly oncogenic group B adeno virus types all induce a common TSTA. Another TSTA is induced by the group C viruses, while no evidence has been obtained for the existence of any TSTA induced by a group D virus type.
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| 9. |
- Ankerst, J., et al.
(författare)
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Induction of mammary fibroadenomas in rats by adenovirus type 9
- 1974
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 13:3, s. 286-290
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Tidskriftsartikel (refereegranskat)abstract
- After inoculation of adenovirus type 9 into newborn Wistar/Furth rats, seven out of seven females developed one or several mammary fibroadenomas within 14–25 weeks after virus inoculation. No tumours were observed in male rats inoculated with the same virus or in untreated controls. Neonatal inoculations of adenovirus type 5, produced on the same HeLa cells, gave negative results in both sexes. The results indicate that benign mammary tumours can be induced in rats by a virus and that mammary fibroadenomas are induced by adenovirus type 9, previously known to be capable of transforming cells in vitro but not of inducing tumours in vivo.
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| 10. |
- Ankerst, Jaro, et al.
(författare)
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Inhibitory effects of BCG on adenovirus tumorigenesis : Dependence on administration schedule
- 1972
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 10:2, s. 351-356
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Tidskriftsartikel (refereegranskat)abstract
- The cumulative incidence of tumours was registered in CBA mice inoculated with adenovirus type 12 when newborn and treated with BCG in different ways during the latency period. A single subcutaneous dose of BCG at 3–4 weeks of age had a preventive effect, while no prevention could be recorded after a single BCG dose at 9 weeks of age or eight BCG injections at intervals of 1 week. Sera obtained during the latency period were tested for capacity to block lymph‐node cell‐mediated tumour immunity. Blocking activity was detectable in pooled serum of the mice which had been infected with BCG at 9 weeks of age, already at 10 weeks, i.e. 1 to 2 weeks before the appearance of palpable tumours. Blocking activity could not be demonstrated in a serum pool from control mice until 14 weeks after virus inoculation, at which time sera from mice inoculated with BCG at the age of 3 weeks were still negative. The results indicate that the effect of BCG upon tumour development is dependent upon the point of time of BCG treatment and further suggest that BCG treatment initiated at a stage when serum blocking activity is already present can further stimulate the production of blocking factors and tentatively promote tumour development.
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