| 1. |
- Granholm, Fredrik, et al.
(författare)
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A Feasibility Study on the Identification of Potential Biomarkers in Pulmonary Embolism Using Proteomic Analysis
- 2022
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Ingår i: Clinical and applied thrombosis/hemostasis. - : SAGE Publications. - 1076-0296 .- 1938-2723. ; 28
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Tidskriftsartikel (refereegranskat)abstract
- Acute pulmonary embolism (PE) is a common emergency with a high morbidity and mortality. Most clinical presentations are non-specific and there is a lack of suitable biomarkers for PE. For example, the traditional D-dimer tests shows a rather high sensitivity for PE, but yet a rather low positive predictive value due to its lack of specificity. Research on novel biomarkers for PE is thus of interest to improve early diagnostics and reduce the number of unnecessary computed tomography pulmonary angiogram (CTPA) scans performed. In this study we evaluate the feasibility to use label-free quantitative proteomics to discover potential biomarkers for acute PE and to monitor changes in proteins levels in PE patients over time. Blood was collected from 8 patients with CTPA verified PE and from 8 patients presenting with same symptoms but with a negative CTPA. The samples were analyzed by liquid chromatography-mass spectrometry and thirteen protein concentrations were found to be significantly changed in PE patients compared to the CTPA negative controls. This exploratory study shows that proteomic analysis can be used to identify potential biomarkers for PE as well as to monitor changes of protein levels over time. The complement proteins play a part in PE but further studies are needed to clarify their specific role in the pathophysiological process and to look for more specific proteins.
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| 2. |
- Wedin, Johan O., et al.
(författare)
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Impact of training specificity on exercise-induced cardiac troponin elevation in professional athletes : A pilot study
- 2020
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Ingår i: World Journal of Cardiology. - : Baishideng Publishing Group Inc.. - 1949-8462. ; 12:1, s. 35-43
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND Release of cardiac biomarkers is common after strenuous endurance exercise, but data on intermittent exercise are scarce. It has not been investigated whether cardiac troponin elevation is influenced depending on the type of exercise that an athlete is adapted to perform. We hypothesized that intermittent but not continuous exercise induces cardiac troponin elevation in professional athletes adapted to high-intensity intermittent exercise. AIM To examine how training specificity impacts high-sensitivity cardiac troponin T (hs-cTnT) release. METHODS Nine professional floorball players participated in the study, which comprised two different exercise tests: a continuous incremental cycle ergometer test and a Yo-Yo Intermittent Recovery 2 (Yo-Yo IR2) test. Serial assessment of hs-cTnT was performed after the cycle ergometer test and the Yo-Yo IR2 test (baseline, 0, 2, 6, and 24 h). RESULTS No hs-cTnT elevation above the myocardial damage cutoff (>= 14 ng/L) was shown after the cycle ergometer test, whereas hs-cTnT levels rose over the cutoff in three of nine participants after the Yo-Yo IR2 test. The hs-cTnT levels peaked at 6 h after both tests, but were significantly higher after the Yo-Yo IR2 test compared to the cycle ergometer test (median hs-cTnT concentration 10.6 ng/L vs 7.8 ng/L, P = 0.038). All levels returned to baseline within 24 h. CONCLUSION In professional athletes adapted to high-intensity intermittent exercise, hs-cTnT was significantly elevated after intermittent but not continuous exercise. This principle of specificity training should be considered when designing future studies to avoid misinterpretation of hs-cTnT elevation.
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| 3. |
- Wedin, Johan O., et al.
(författare)
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The Influence of Floorball on Hematological Parameters : Consequences in Health Assessment and Antidoping Testing
- 2020
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Ingår i: Journal of Sports Medicine. - : Hindawi Limited. - 2356-7651 .- 2314-6176.
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Tidskriftsartikel (refereegranskat)abstract
- Assessment of hematological parameters is common in sports medicine. Although physical exercise is an important preanalytical variable, data about acute hematological changes after high-intensity intermittent exercise are scarce. This study aimed to examine floorball as a potential preanalytical variable for hematological parameters used in health assessment and antidoping testing. Twenty-three professional male floorball players participated in a floorball game. Hematological parameters including hemoglobin, erythrocyte count and erythrocyte indices, reticulocytes, white blood cells (WBC), platelets, reticulocytes, and OFF-hr score were assessed at baseline, immediately postgame, and at 2 h postgame. Median hemoglobin concentration decreased significantly from 146 g/L pregame to 141 g/L immediately postgame (). WBC count increased from 7.2 × 109/L pregame to 10.1 × 109/L 2 h postgame (). The median OFF-hr score decreased from 99.5 to 94.2 immediately postgame and remained significantly lower than baseline at 2 h postgame (94.4, ). Looking at individual results, the highest OFF-hr score increased from 120 at baseline to 124 at 2 h postgame. Our findings suggest that participation in a floorball game affects several hematological parameters and consequently can affect health assessment and antidoping testing.
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| 4. |
- Henriksson, Anders E., et al.
(författare)
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Identification of Potential Plasma Biomarkers for Abdominal Aortic Aneurysm Using Tandem Mass Tag Quantitative Proteomics
- 2018
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Ingår i: Proteomes. - : MDPI AG. - 2227-7382. ; 6:4
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Tidskriftsartikel (refereegranskat)abstract
- Plasma biomarkers that identify abdominal aortic aneurysm (AAA) rupture risk would greatly assist in stratifying patients with small aneurysms. Identification of such biomarkers has hitherto been unsuccessful over a range of studies using different methods. The present study used an alternative proteomic approach to find new, potential plasma AAA biomarker candidates. Pre-fractionated plasma samples from twelve patients with AAA and eight matched controls without aneurysm were analyzed by mass spectrometry applying a tandem mass tag (TMT) technique. Eight proteins were differentially regulated in patients compared to controls, including decreased levels of the enzyme bleomycin hydrolase. The down-regulation of this enzyme was confirmed in an extended validation study using an enzyme-linked immunosorbent assay (ELISA). The TMT-based proteomic approach thus identified novel potential plasma biomarkers for AAA.
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| 5. |
- Lindström, Joakim, et al.
(författare)
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The agreement between capillary and venous sampling for plasma PT/INR results
- 2018
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Ingår i: Scandinavian Journal of Clinical and Laboratory Investigation. - : Informa UK Limited. - 0036-5513 .- 1502-7686. ; 78:6, s. 524-526
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Tidskriftsartikel (refereegranskat)abstract
- Regular measurement of prothrombin time (PT) as an international normalized ratio (INR) is of great importance in ensuring a safe Warfarin treatment due to the narrow therapeutic range. Hence, accurate results of the PT/INR analysis are crucial. Due to the high sampling load that patients on Warfarin experience, some prefer capillary sampling (from the tip of the finger) as an alternative to standard venous sampling (from the cubital fossa). The present study evaluated the method of capillary sampling compared to the standard venous method for plasma PT/INR measurement. Both venous and capillary samples were collected simultaneously for plasma PT/INR testing from 100 patients of which the majority undergoing active Warfarin treatment. The samples were analyzed on a Sysmex 2100i instrument (Siemens) using the Owrens method with PT reagents from MediRox. The capillary sampling method showed statistically significant lower values (p <.001) for plasma PT/INR than the standard venous sampling method with a mean difference (bias) of –0.14. It is improbable that the difference (bias) between capillary and venous sampling for plasma PT/INR analysis has clinical relevance. However, since the capillary blood collection and handling for plasma PT/INR is more technically demanding than the standard venous method we recommended that only specially trained personnel be entrusted to draw capillary blood for plasma PT/INR analysis.
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| 6. |
- Röijezon, Ulrik, et al.
(författare)
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An Initial Study on the Coordination of Rod and Line Hauling Movements in Distance Fly Casting
- 2017
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Ingår i: Annals of Applied Sport Science. - : Asian Exercise and Sport Science Association. - 2476-4981 .- 2322-4479. ; 5:2, s. 61-72
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Tidskriftsartikel (refereegranskat)abstract
- Background. The double haul is a unique feature of single-handed fly casting and is used in both fly fishing and fly casting competition. The movement behaviour during the double haul has not been investigated in previous research.Objectives. Describe the coordination of the rod and line hauling movements during distance fly casting.Methods. Elite fly casters performed distance casting with four different fly rod and fly line set-ups used in fly fishing and fly casting competition. Rod and hauling movements were measured with a 3D motion analysis system.Results. The rod and line hauling movements were coordinated in an order whereby peak translational speed of the rod occurs prior to the peak speed of the angular rotation of the rod, and the peak speed of the angular rotation of the rod occurs prior to the peak speed of the line haul. This was consistent for all cast sequences, i.e., the back and forward false casts and the delivery cast, and for all four equipment set-ups, i.e., a shooting-head line cast with a relatively stiff fly rod and a long-belly line cast with three different fly rods with different stiffness and action curves. Results also showed differences in movement coordination between cast sequences and rod and line set-ups.Conclusion. Among elite casters, single-handed fly casting with double haul is coordinated in an order of events whereby the peak speed occurs first for the translation of the rod, then for the rotation of the rod and finally for the line haul.
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| 7. |
- Henriksson, Anders E.
(författare)
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S100B and the influence of seasonal variation
- 2016
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Ingår i: Scandinavian Journal of Clinical and Laboratory Investigation. - : Informa UK Limited. - 0036-5513 .- 1502-7686. ; 76:4, s. 338-340
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Tidskriftsartikel (refereegranskat)abstract
- Background A blood test for S100B can be used to rule out intracranial complications after minor head injury and thereby reduce the need for computed tomography (CT) examinations. The aim of this study was to investigate the clinical importance of a possible influence of seasonal variation on S100B. Methods The individual seasonal variation of S100B in 69 healthy volunteers living at latitudes with extremely variable seasonal exposure to sunlight was investigated. Results The mean serum concentration of S100B was 13% higher in August than in February, but however, not statistically significant (p = 0.068). A good agreement between summer and winter S100B values was confirmed by Bland-Altman analysis and a significant correlation (r = 0.317, p = 0.008) was shown between summer and winter S100B values. Conclusion This study did not show any clinical importance of seasonal variation of S100B that may influence the decision of CT scanning patients with head injuries.
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| 8. |
- Piirainen, Robert, et al.
(författare)
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The impact of seasonal variation of 25-hydroxyvitamin D and parathyroid hormone on calcium levels
- 2016
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Ingår i: Clinical Biochemistry. - : Elsevier BV. - 0009-9120 .- 1873-2933. ; 49:12, s. 850-853
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Tidskriftsartikel (refereegranskat)abstract
- Background: Primary hyperparathyroidism is often diagnosed by high calcium levels in blood. It is well known that calcium levels are dependent on vitamin D and Parathyroid hormone (PTH). Since vitamin D has a seasonal variation the calcium levels might also be influenced by seasonal variation. If a seasonal variation in calcium levels exists, this must be considered in the investigation of suspected hyperparathyroidism. The aim of the present study was to investigate the possible influence and magnitude of the seasonal variation of vitamin D and PTH on calcium levels. Method: In the present study the individual seasonal variation of 25-hydroxyvitamin D [25(OH) D], PTH and calcium in 69 healthy volunteers living at latitudes with extremely variable seasonal exposure to sunlight have been investigated. Results: As expected the 25(OH) D levels were significantly higher (42%) in summer compared to winter. PTH levels were significantly lower (7%) in summer than in winter. The mean serum concentration of calcium was 1% higher in August than in February, however not statistically significant. A good agreement between summer and winter calcium values was confirmed by Bland-Altman analysis. Conclusion: This study did not show any clinically important influence of seasonal variation of 25(OH) D and PTH on calcium that may influence a clinician's decision to investigate suspected hyperparathyroidism.
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| 9. |
- Antinyan, Vard, 1984, et al.
(författare)
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Identifying Complex Functions : By Investigating Various Aspects of Code Complexity
- 2015
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Ingår i: Proceedings of 2015 Science and Information Conference (SAI). - : IEEE Press. - 9781479985470 - 9781479985487 - 9781479985463 ; , s. 879-888
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Konferensbidrag (refereegranskat)abstract
- The complexity management of software code has become one of the major problems in software development industry. With growing complexity the maintenance effort of code increases. Moreover, various aspects of complexity create difficulties for complexity assessment. The objective of this paper is to investigate the relationships of various aspects of code complexity and propose a method for identifying the most complex functions. We have conducted an action research project in two software development companies and complemented it with a study of three open source products. Four complexity metrics are measured, and their nature and mutual influence are investigated. The results and possible explanations are discussed with software engineers in industry. The results show that there are two distinguishable aspects of complexity of source code functions: Internal and outbound complexities. Those have an inverse relationship. Moreover, the product of them does not seem to be greater than a certain limit, regardless of software size. We present a method that permits identification of most complex functions considering the two aspects of complexities. The evaluation shows that the use of the method is effective in industry: It enables identification of 0.5% most complex functions out of thousands of functions for reengineering.
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| 10. |
- Bylund, Dan, et al.
(författare)
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Proteomic approaches to identify circulating biomarkers in patients with abdominal aortic aneurysm
- 2015
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Ingår i: American Journal of Cardiovascular Disease. - : e-Century Publishing Corporation. - 2160-200X. ; 5:3, s. 140-145
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Forskningsöversikt (refereegranskat)abstract
- Abdominal aortic aneurysm (AAA) is a common condition with high mortality when ruptured. Most clinicians agree that small AAAs are best managed by ultrasonographic surveillance. However, it has been stated in recent reviews that a serum/plasma biomarker that predicts AAA rupture risk would be a powerful tool in stratifying patients with small AAAs. Identification of such circulating biomarkers with traditional hypothesis driven studies has been unsuccessful. In this review we summarize six studies using different proteomic approaches to find new, potential plasma AAA biomarker candidates. In conclusion, by using proteomic approaches novel potential plasma biomarkers for AAA have been identified.
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