| 1. |
- Gatenholm, Paul, 1956, et al.
(author)
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Effect of cultivation conditions on the structure and morphological properties of BNC biomaterials with a focus on vascular grafts
- 2016
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In: Bacterial NanoCellulose: A Sophisticated Multifunctional Material. - Boca Raton : CRC Press. - 9781439869925 ; , s. 19-42
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Book chapter (other academic/artistic)abstract
- 20 New materials that are not thrombogenic and have mechanical properties that mimic the native blood vessel are in very great demand. Nanocellulose produced by the bacteria Gluconacetobacter xylinus is a biomaterial that has gained interest in the field of tissue engineering because of its unique properties, such as great mechanical strength, high water content (around 99%), and the ability to be shaped into three-dimensional structures during biosynthesis. The fabrication process of bacterial nanocellulose (BNC) vascular grafts is very unique because the material synthesis and product formation takes place simultaneously. The bio mechanical performance, which includes rupture pressure and compliance along with biological response (endothelialization, blood compatibility, etc.), is dependent on the morphology of a fibrillar network. The network formation is affected by cellulose assembly and bacteria motion, proliferation rate, and other factors. An understanding of the effects of cultivation conditions on BNC network formation is therefore of great importance.
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| 2. |
- Kuzmenko, Volodymyr, 1987, et al.
(author)
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Enhanced growth of neural networks on conductive cellulose-derived nanofibrous scaffolds
- 2016
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In: Materials Science and Engineering C. - : Elsevier BV. - 0928-4931 .- 1873-0191. ; 58, s. 14-23
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Journal article (peer-reviewed)abstract
- The problemof recovery fromneurodegeneration needs new effective solutions. Tissue engineering is viewed as a prospective approach for solving this problemsince it can help to develop healthy neural tissue using supportivescaffolds. This study presents effective and sustainable tissue engineering methods for creating biomaterials from cellulose that can be used either as scaffolds for the growth of neural tissue in vitro or as drug screening models. To reach this goal, nanofibrous electrospun cellulose mats were made conductive via two different procedures: carbonization and addition of multi-walled carbon nanotubes. The resulting scaffolds were much moreconductive than untreated cellulose material and were used to support growth and differentiation of SH-SY5Y neuroblastoma cells. The cells were evaluated by scanning electron microscopy and confocal microscopy methods over a period of 15 days at different time points. The results showed that the cellulose-derived conductive scaffolds can provide support for good cell attachment, growth and differentiation. The formation of a neural network occurred within 10 days of differentiation, which is a promising length of time for SH-SY5Y neuroblastoma cells.
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| 3. |
- Kuzmenko, Volodymyr, 1987, et al.
(author)
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Enhanced growth of neural networks on cellulose-derived carbon nanofibrous scaffolds
- 2015
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In: Annual World Conference on Carbon – CARBON 2015.
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Conference paper (peer-reviewed)abstract
- Tissue engineering is a prospective method for solving the problem of recovery from neurodegenerative disorders as it helps to grow healthy neural tissue using supportive scaffolds. Biocompatible scaffolds with mechanical stability, appropriate topography and electrical conductivity previously demonstrated efficient results in neural tissue engineering applications. In this study, we present sustainable cellulose-derived carbon nanofibrous (CNF) biomaterial that can be used either as a scaffold for the regeneration of neural tissue or as a drug screening model. This scaffold material was characterized with excellent biocompatibility (95.6% cell viability), nanosized topography (fiber diameter in the range of 50-250 nm) and electrical conductivity (10*7 times higher value than the one of an unmodified cellulosic precursor) to support adhesion, growth and differentiation of SH-SY5Y neuroblastoma cells. The results showed that the formation of a neural network occurred within 10 days of differentiation, which is a good duration for SH-SY5Y neuroblastoma cells. We can conclude that topography and electrical conductivity of the CNF material played a major role in its positive influence on the development of neural tissue. CNF nanotopography resembles the one of an extracellular matrix of neural tissue, while electrical conductivity allows utilization of electrochemical signals for information transmission between neurons.
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| 4. |
- Thunberg, Johannes, 1982, et al.
(author)
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In situ synthesis of conductive polypyrrole on electrospun cellulose nanofibers: scaffold for neural tissue engineering
- 2015
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In: Cellulose. - : Springer Science and Business Media LLC. - 0969-0239 .- 1572-882X. ; 22:3, s. 1459-1467
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Journal article (peer-reviewed)abstract
- This study reports the synthesis of conductive polypyrrole (PPy) on electrospun cellulose nanofibers. The cellulose nanofibers were electrospun via cellulose acetate and surface modified using in situ pyrrole polymerization. PPy adhered to the cellulose nanofiber surface as small particles and caused a 105 fold increase in conductivity compared to unmodified cellulose nanofibers. In addition, tests revealed no cytotoxic potential for the PPy coated cellulose nanofiber materials. In vitro culturing using SH-SY5Y human neuroblastoma cells indicated enhanced cell adhesion on the PPy coated cellulose material. SH-SY5Y cell viability was evident up to 15 days of differentiation and cells adhered to the PPy coated cellulose nanofibers and altered their morphology to a more neuron like phenotype.
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| 5. |
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| 6. |
- Innala, Marcus, et al.
(author)
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3D Culturing and differentiation of SH-SY5Y neuroblastoma cells on bacterial nanocellulose scaffolds.
- 2014
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In: Artificial cells, nanomedicine, and biotechnology (Print). - : Informa UK Limited. - 2169-141X .- 2169-1401. ; 42:5, s. 302-308
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Journal article (peer-reviewed)abstract
- A new in vitro model, mimicking the complexity of nerve tissue, was developed based on a bacterial nanocellulose (BNC) scaffold that supports 3D culturing of neuronal cells. BNC is extracellularly excreted by Gluconacetobacter xylinus (G. xylinus) in the shape of long non-aggregated nanofibrils. The cellulose network created by G. xylinus has good mechanical properties, 99% water content, and the ability to be shaped into 3D structures by culturing in different molds. Surface modification with trimethyl ammonium beta-hydroxypropyl (TMAHP) to induce a positive surface charge, followed by collagen I coating, has been used to improve cell adhesion, growth, and differentiation on the scaffold. In the present study, we used SH-SY5Y neuroblastoma cells as a neuronal model. These cells attached and proliferated well on the BNC scaffold, as demonstrated by scanning electron microscopy (SEM) and the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay. Following neuronal differentiation, we demonstrated functional action potentials (APs) by electrophysiological recordings, indicating the presence of mature neurons on the scaffolds. In conclusion, we have demonstrated for the first time that neurons can attach, proliferate, and differentiate on BNC. This 3D model based on BNC scaffolds could possibly be used for developing in vitro disease models, when combined with human induced pluripotent stem (iPS) cells (derived from diseased patients) for detailed investigations of neurodegenerative disease mechanisms and in the search for new therapeutics.
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